| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-81 |
Sentence |
denotes |
Growth-inhibitory effects of MOB2 on human hepatic carcinoma cell line SMMC-7721. |
| TextSentencer_T2 |
82-86 |
Sentence |
denotes |
AIM: |
| TextSentencer_T3 |
87-219 |
Sentence |
denotes |
To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721. |
| TextSentencer_T4 |
220-228 |
Sentence |
denotes |
METHODS: |
| TextSentencer_T5 |
229-325 |
Sentence |
denotes |
The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells. |
| TextSentencer_T6 |
326-421 |
Sentence |
denotes |
The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1. |
| TextSentencer_T7 |
422-555 |
Sentence |
denotes |
After lipofection of the MOB2 gene into cancer cells, the levels of MOB2 protein in the cancer cells were detected by immunoblotting. |
| TextSentencer_T8 |
556-825 |
Sentence |
denotes |
To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells, the cells were cultured in Dulbecco's Modified Eagle's Medium with 10% fetal calf serum and glutamine, and then mixed with liposomes, Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2. |
| TextSentencer_T9 |
826-834 |
Sentence |
denotes |
RESULTS: |
| TextSentencer_T10 |
835-991 |
Sentence |
denotes |
We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry. |
| TextSentencer_T11 |
992-1138 |
Sentence |
denotes |
We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2. |
| TextSentencer_T12 |
1139-1333 |
Sentence |
denotes |
After transfection, MOB2 enhanced growth suppression, induced apoptosis, increased the ratio of G0/G1, significantly inhibited the advance of cell cycle phase, and arrested cells in G0/G1 phase. |
| TextSentencer_T13 |
1334-1497 |
Sentence |
denotes |
CONCLUSION: MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells, which may be useful in gene therapy for hepatic carcinoma. |
| T1 |
0-81 |
Sentence |
denotes |
Growth-inhibitory effects of MOB2 on human hepatic carcinoma cell line SMMC-7721. |
| T2 |
82-86 |
Sentence |
denotes |
AIM: |
| T3 |
87-219 |
Sentence |
denotes |
To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721. |
| T4 |
220-228 |
Sentence |
denotes |
METHODS: |
| T5 |
229-325 |
Sentence |
denotes |
The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells. |
| T6 |
326-421 |
Sentence |
denotes |
The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1. |
| T7 |
422-555 |
Sentence |
denotes |
After lipofection of the MOB2 gene into cancer cells, the levels of MOB2 protein in the cancer cells were detected by immunoblotting. |
| T8 |
556-825 |
Sentence |
denotes |
To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells, the cells were cultured in Dulbecco's Modified Eagle's Medium with 10% fetal calf serum and glutamine, and then mixed with liposomes, Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2. |
| T9 |
826-834 |
Sentence |
denotes |
RESULTS: |
| T10 |
835-991 |
Sentence |
denotes |
We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry. |
| T11 |
992-1138 |
Sentence |
denotes |
We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2. |
| T12 |
1139-1333 |
Sentence |
denotes |
After transfection, MOB2 enhanced growth suppression, induced apoptosis, increased the ratio of G0/G1, significantly inhibited the advance of cell cycle phase, and arrested cells in G0/G1 phase. |
| T13 |
1334-1497 |
Sentence |
denotes |
CONCLUSION: MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells, which may be useful in gene therapy for hepatic carcinoma. |