PubMed:23321162 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/23321162","sourcedb":"PubMed","sourceid":"23321162","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/23321162","text":"Prognostic significance of PUMA in pancreatic ductal adenocarcinoma.\nOBJECTIVES: To investigate retrospectively whether alterations of p53 upregulated mediator of apoptosis (PUMA) protein levels and somatic mutations of the PUMA gene are characteristic of pancreatic ductal adenocarcinoma (PDAC).\nMETHODS: Immunohistochemical analyses of PUMA were performed in pancreatic tumour tissue samples, and paired normal pancreatic tissue samples, from patients with PDAC. Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labelling assay.\nRESULTS: A total of 70 patients with PDAC had samples available; 49 cases (70.0%) had high PUMA protein levels. PUMA was not detected in paired normal tissue samples. Significantly higher levels of PUMA protein were detected in low-grade tumours (tumour -node-metastasis stages I and II), compared with higher grade (stage III) tumours. Of the PDAC cases, the mean apoptosis index value for PUMA-positive specimens was significantly higher than that for PUMA-negative specimens. Overall survival was significantly associated with PUMA immunoreactivity.\nCONCLUSIONS: High levels of PUMA in PDAC tumour cells suggest that PUMA expression may play a role in pancreatic tumourigenesis.","tracks":[{"project":"Allie","denotations":[{"id":"SS1_23321162_2_0","span":{"begin":256,"end":288},"obj":"expanded"},{"id":"SS2_23321162_2_0","span":{"begin":290,"end":294},"obj":"abbr"}],"relations":[{"id":"AE1_23321162_2_0","pred":"abbreviatedTo","subj":"SS1_23321162_2_0","obj":"SS2_23321162_2_0"}],"attributes":[{"subj":"SS1_23321162_2_0","pred":"source","obj":"Allie"},{"subj":"SS2_23321162_2_0","pred":"source","obj":"Allie"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":199,"end":216},"obj":"HP_0001428"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":791,"end":795},"obj":"gene:27113"},{"id":"T1","span":{"begin":853,"end":863},"obj":"disease:C0027627"},{"id":"T2","span":{"begin":791,"end":795},"obj":"gene:27113"},{"id":"T3","span":{"begin":853,"end":863},"obj":"disease:C2939420"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Allie","color":"#afec93","default":true},{"id":"PubmedHPO","color":"#ec93c9"},{"id":"DisGeNET","color":"#93e4ec"}]}]}}