PubMed:23319345 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_23319345_1_0","span":{"begin":148,"end":161},"obj":"expanded"},{"id":"SS2_23319345_1_0","span":{"begin":163,"end":168},"obj":"abbr"},{"id":"SS1_23319345_1_1","span":{"begin":261,"end":270},"obj":"expanded"},{"id":"SS2_23319345_1_1","span":{"begin":272,"end":275},"obj":"abbr"}],"relations":[{"id":"AE1_23319345_1_0","pred":"abbreviatedTo","subj":"SS1_23319345_1_0","obj":"SS2_23319345_1_0"},{"id":"AE1_23319345_1_1","pred":"abbreviatedTo","subj":"SS1_23319345_1_1","obj":"SS2_23319345_1_1"}],"text":"Effect of RNAi-mediated knockdown of the Bombyx mori transformer-2 gene on the sex-specific splicing of Bmdsx pre-mRNA.\nIn Drosophila melanogaster, transformer-2 (tra-2) is essential for female differentiation and is known to induce female-specific splicing of doublesex (dsx). The function of Bmtra-2, the Bombyx mori homolog of tra-2, on the other hand remains to be elucidated. As an initial step to learn about the biological function of Bmtra-2, we determined whether Bmtra-2 is capable of inducing the female-specific splicing of Drosophila dsx. RNAi-mediated knockdown of Bmtra-2 using Bombyx cultured cells transiently transfected with a dsx minigene revealed that Bmtra-2 can induce female-specific splicing of Drosophila dsx. To examine the role Bmtra-2 plays in regulating sex-specific splicing of Bmdsx pre-mRNA, we used an RNAi approach to reduce BmTra-2 expression in the early embryo. Embryos injected with dsRNAs or siRNAs targeted to Bmtra-2 showed no variation in the sex-specific splicing pattern of Bmdsx pre-mRNA. RNAi knockdown of Bmtra-2 in the early embryo caused abnormal testis formation. Taken together, these results indicate that Bmtra-2 is required for normal testis development, but is not involved in regulating the sex-specific splicing of Bmdsx pre-mRNA, even though it is capable of inducing the female-specific splicing of Drosophila dsx."}