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PubMed:23250425
Annnotations
silkwormbase
{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":246,"end":253},"obj":"Chemical:MESH:D008543"},{"id":"T2","span":{"begin":369,"end":377},"obj":"Species:7091"},{"id":"T3","span":{"begin":379,"end":390},"obj":"Species:7091"},{"id":"T4","span":{"begin":793,"end":800},"obj":"Species:7091"},{"id":"T5","span":{"begin":481,"end":486},"obj":"BMO_00497"},{"id":"T6","span":{"begin":831,"end":839},"obj":"Gene:100270765"},{"id":"T7","span":{"begin":640,"end":678},"obj":"Gene:780849"},{"id":"T8","span":{"begin":976,"end":1014},"obj":"Gene:780849"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
silkworm_phenotype
{"project":"silkworm_phenotype","denotations":[{"id":"T8","span":{"begin":976,"end":1014},"obj":"Gene:780849"},{"id":"T7","span":{"begin":640,"end":678},"obj":"Gene:780849"},{"id":"T6","span":{"begin":831,"end":839},"obj":"Gene:100270765"},{"id":"T5","span":{"begin":481,"end":486},"obj":"BMO_00497"},{"id":"T4","span":{"begin":793,"end":800},"obj":"Species:7091"},{"id":"T3","span":{"begin":379,"end":390},"obj":"Species:7091"},{"id":"T2","span":{"begin":369,"end":377},"obj":"Species:7091"},{"id":"T1","span":{"begin":246,"end":253},"obj":"Chemical:MESH:D008543"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
silkworm
{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":246,"end":253},"obj":"Chemical:MESH:D008543"},{"id":"T2","span":{"begin":369,"end":377},"obj":"Species:7091"},{"id":"T3","span":{"begin":379,"end":390},"obj":"Species:7091"},{"id":"T4","span":{"begin":793,"end":800},"obj":"Species:7091"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
Allie
{"project":"Allie","denotations":[{"id":"SS1_23250425_5_0","span":{"begin":793,"end":829},"obj":"expanded"},{"id":"SS2_23250425_5_0","span":{"begin":831,"end":839},"obj":"abbr"}],"relations":[{"id":"AE1_23250425_5_0","pred":"abbreviatedTo","subj":"SS1_23250425_5_0","obj":"SS2_23250425_5_0"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
Anatomy-MAT
{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":211,"end":214},"obj":"Body_part"},{"id":"T2","span":{"begin":254,"end":262},"obj":"Body_part"},{"id":"T3","span":{"begin":624,"end":636},"obj":"Body_part"},{"id":"T4","span":{"begin":710,"end":723},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000140"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000159"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000159"},{"id":"A4","pred":"mat_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MAT_0000400"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
NCBITAXON
{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":369,"end":377},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":379,"end":390},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":1049,"end":1057},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1065,"end":1082},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":1087,"end":1096},"obj":"OrganismTaxon"},{"id":"T7","span":{"begin":1097,"end":1120},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"7091"},{"id":"A2","pred":"db_id","subj":"T2","obj":"7091"},{"id":"A3","pred":"db_id","subj":"T3","obj":"7080"},{"id":"A4","pred":"db_id","subj":"T4","obj":"115357"},{"id":"A5","pred":"db_id","subj":"T5","obj":"7211"},{"id":"A6","pred":"db_id","subj":"T5","obj":"7215"},{"id":"A7","pred":"db_id","subj":"T7","obj":"7227"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}
Anatomy-UBERON
{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":211,"end":214},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000019"},{"id":"A2","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000970"},{"id":"A3","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0010230"}],"text":"A visible dominant marker for insect transgenesis.\nTransgenesis of most insects currently relies on fluorescence markers. Here we establish a transformation marker system causing phenotypes visible to the naked eye due to changes in the color of melanin pigments, which are widespread in animals. Ubiquitous overexpression of arylalkylamine-N-acetyl transferase in the silkworm, Bombyx mori, changes the color of newly hatched first-instar larvae from black to a distinctive light brown color, and can be used as a molecular marker by directly connecting to baculovirus immediate early 1 gene promoter. Suppression of black pigmentation by Bm-arylalkylamine-N-acetyl transferase can be observed throughout the larval stages and in adult animals. Alternatively, overexpression in another gene, B. mori β-alanyl-dopamine synthetase (Bm-ebony), changes the larval body color of older instars, although first-instar larvae had normal dark coloration. We further show that ectopic Bm-arylalkylamine-N-acetyl transferase expression lightens coloration in ladybird beetle Harmonia axyridis and fruit fly Drosophila melanogaster, highlighting the potential usefulness of this marker for transgenesis in diverse insect taxa."}