PubMed:2303438 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2303438","sourcedb":"PubMed","sourceid":"2303438","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2303438","text":"The S49 Kin- cell line transcribes and translates a functional mRNA coding for the catalytic subunit of cAMP-dependent protein kinase.\nThe S49 mouse lymphoma mutant cell line Kin- is resistant to the cytotoxic effects of elevated cAMP levels, has no detectable cAMP-dependent protein kinase activity, and has depressed levels of cAMP-binding regulatory subunits. We demonstrate that although the Kin- cell line lacks detectable catalytic subunit protein, these cells express wild-type levels of mRNA for both C alpha and C beta catalytic subunit isoforms. Translation of C alpha mRNA appears to be normal in the Kin- cell, based on the observation that C alpha mRNA associates with large polyribosomes in both wild-type and Kin- cells. We cloned the C alpha cDNA from Kin- cells and show that its transient expression in another cell type leads to activation of a cAMP-sensitive luciferase reporter gene, suggesting that functional C alpha protein is made. In addition to having catalytic activity, the C alpha subunit from Kin- cells is inhibited in the presence of mouse RI alpha regulatory subunit, indicating that formation of the holoenzyme complex is normal. We suggest that the mutation responsible for the Kin- phenotype is in a cellular component that directly or indirectly causes Kin- catalytic subunit protein to be degraded rapidly.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":149,"end":157},"obj":"HP_0002665"},{"id":"T2","span":{"begin":309,"end":318},"obj":"HP_0000716"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"}]},{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":134},"obj":"Sentence"},{"id":"T2","span":{"begin":135,"end":362},"obj":"Sentence"},{"id":"T3","span":{"begin":363,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":735},"obj":"Sentence"},{"id":"T5","span":{"begin":736,"end":956},"obj":"Sentence"},{"id":"T6","span":{"begin":957,"end":1164},"obj":"Sentence"},{"id":"T7","span":{"begin":1165,"end":1345},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":134},"obj":"Sentence"},{"id":"T2","span":{"begin":135,"end":362},"obj":"Sentence"},{"id":"T3","span":{"begin":363,"end":555},"obj":"Sentence"},{"id":"T4","span":{"begin":556,"end":735},"obj":"Sentence"},{"id":"T5","span":{"begin":736,"end":956},"obj":"Sentence"},{"id":"T6","span":{"begin":957,"end":1164},"obj":"Sentence"},{"id":"T7","span":{"begin":1165,"end":1345},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":143,"end":148},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":1067,"end":1072},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"},{"id":"A3","pred":"db_id","subj":"T3","obj":"10088"},{"id":"A4","pred":"db_id","subj":"T3","obj":"10090"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"}]},{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":149,"end":157},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005062"},{"subj":"T1","pred":"source","obj":"mondo_disease"}]},{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":149,"end":157},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002665"},{"subj":"T1","pred":"source","obj":"HP-phenotype"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#93d8ec","default":true},{"id":"sentences","color":"#ece593"},{"id":"NCBITAXON","color":"#cb93ec"},{"id":"mondo_disease","color":"#93ecb1"},{"id":"HP-phenotype","color":"#ec9793"}]}]}}