PubMed:22976301 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/22976301","sourcedb":"PubMed","sourceid":"22976301","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/22976301","text":"Drosophila Cep135/Bld10 maintains proper centriole structure but is dispensable for cartwheel formation.\nCep135/Bld10 is a conserved centriolar protein required for the formation of the central cartwheel, an early intermediate in centriole assembly. Surprisingly, Cep135/Bld10 is not essential for centriole duplication in Drosophila, suggesting either that Cep135/Bld10 is not essential for cartwheel formation, or that the cartwheel is not essential for centriole assembly in flies. Using electron tomography and super-resolution microscopy we show that centrioles can form a cartwheel in the absence of Cep135/Bld10, but centriole width is increased and the cartwheel appears to disassemble over time. Using 3D structured illumination microscopy we show that Cep135/Bld10 is localized to a region between inner (SAS-6, Ana2) and outer (Asl, DSpd-2 and D-PLP) centriolar components, and the localization of all these component is subtly perturbed in the absence of Cep135/Bld10, although the ninefold symmetry of the centriole is maintained. Thus, in flies, Cep135/Bld10 is not essential for cartwheel assembly or for establishing the ninefold symmetry of centrioles; rather, it appears to stabilize the connection between inner and outer centriole components.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":308,"end":319},"obj":"HP_0009609"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#ece093","default":true}]}]}}