PubMed:22950840 JSONTXT

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":158,"end":171},"obj":"HP_0001427"}],"text":"Isotyping the human TOMM40 variable-length polymorphism by gene amplification and restriction digest.\nRecent studies have shown that the translocase of outer mitochondrial membrane 40 homolog (TOMM40) contains a polymorphic poly-T variant, the long variant of which is associated with an increase in AD incidence among APOE 3 carriers. Current methods to isotype the poly-T region rely on long PCR, subcloning and sequencing to distinguish among the allelic variants. While such methods are extremely accurate as well as quantitative in determining the number of T residues in the poly-T region, the process can be cumbersome, time consuming and expensive to employ in routine laboratories. To this end, we have developed a quick and simple method to isotype the human TOMM40 variable length polymorphism using a PCR- and restriction digest-based approach, enabling rapid genotyping of TOMM40 variants."}

{"project":"Allie","denotations":[{"id":"SS1_22950840_1_0","span":{"begin":137,"end":191},"obj":"expanded"},{"id":"SS2_22950840_1_0","span":{"begin":193,"end":199},"obj":"abbr"}],"relations":[{"id":"AE1_22950840_1_0","pred":"abbreviatedTo","subj":"SS1_22950840_1_0","obj":"SS2_22950840_1_0"}],"text":"Isotyping the human TOMM40 variable-length polymorphism by gene amplification and restriction digest.\nRecent studies have shown that the translocase of outer mitochondrial membrane 40 homolog (TOMM40) contains a polymorphic poly-T variant, the long variant of which is associated with an increase in AD incidence among APOE 3 carriers. Current methods to isotype the poly-T region rely on long PCR, subcloning and sequencing to distinguish among the allelic variants. While such methods are extremely accurate as well as quantitative in determining the number of T residues in the poly-T region, the process can be cumbersome, time consuming and expensive to employ in routine laboratories. To this end, we have developed a quick and simple method to isotype the human TOMM40 variable length polymorphism using a PCR- and restriction digest-based approach, enabling rapid genotyping of TOMM40 variants."}