PubMed:22833313
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-92 | Sentence | denotes | Characterization of the CDP-D-mannitol biosynthetic pathway in Streptococcus pneumoniae 35A. |
| TextSentencer_T2 | 93-179 | Sentence | denotes | Streptococcus pneumoniae is a major human pathogen associated with diseases worldwide. |
| TextSentencer_T3 | 180-368 | Sentence | denotes | The capsular polysaccharides (CPSs) are considered a major virulence factor and are targets for a vaccine. d-Mannitol was found to be present in the CPS of several S. pneumoniae serotypes. |
| TextSentencer_T4 | 369-552 | Sentence | denotes | Two genes, mnp1 and mnp2, which are located in the CPS gene cluster, were proposed to be responsible for the synthesis of NDP-d-mannitol (the nucleotide activated form of d-mannitol). |
| TextSentencer_T5 | 553-677 | Sentence | denotes | However, the pathway has never been identified by experimental methods and we aimed to characterize it in the present study. |
| TextSentencer_T6 | 678-853 | Sentence | denotes | To achieve this, the two genes, mnp1 and mnp2, were cloned and the gene products were overexpressed, purified, and analyzed in vitro for their respective enzymatic activities. |
| TextSentencer_T7 | 854-1052 | Sentence | denotes | Products of reactions catalyzed by Mnp1 and Mnp2 were detected by capillary electrophoresis and validated using electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. |
| TextSentencer_T8 | 1053-1253 | Sentence | denotes | We show that Mnp1 is responsible for the transfer of CMP from CTP to d-fructose-6-phosphate (Fru-6-P) to form CDP-d-fructose, whereas Mnp2 catalyzed the conversion of CDP-d-fructose to CDP-d-mannitol. |
| TextSentencer_T9 | 1254-1421 | Sentence | denotes | Therefore, Mnp1 (renamed as mnpA) was identified as Fru-6-P cytidylyltransferase-encoding gene, and mnp2 (renamed as mnpB) as a CDP-d-fructose reductase-encoding gene. |
| TextSentencer_T10 | 1422-1591 | Sentence | denotes | The kinetics of Mnp1 for the substrate (Fru-6-P and CTP) and of Mnp2 for the substrate (CDP-d-fructose) and the cofactor NADH or NADPH fitted the Michaelis-Menten model. |
| TextSentencer_T11 | 1592-1668 | Sentence | denotes | The effects of temperature, pH and cations on the two enzymes were analyzed. |
| TextSentencer_T12 | 1669-1774 | Sentence | denotes | This is the first time that the biosynthetic pathway of CDP-d-mannitol has been identified biochemically. |
| T1 | 0-92 | Sentence | denotes | Characterization of the CDP-D-mannitol biosynthetic pathway in Streptococcus pneumoniae 35A. |
| T2 | 93-179 | Sentence | denotes | Streptococcus pneumoniae is a major human pathogen associated with diseases worldwide. |
| T3 | 180-368 | Sentence | denotes | The capsular polysaccharides (CPSs) are considered a major virulence factor and are targets for a vaccine. d-Mannitol was found to be present in the CPS of several S. pneumoniae serotypes. |
| T4 | 369-552 | Sentence | denotes | Two genes, mnp1 and mnp2, which are located in the CPS gene cluster, were proposed to be responsible for the synthesis of NDP-d-mannitol (the nucleotide activated form of d-mannitol). |
| T5 | 553-677 | Sentence | denotes | However, the pathway has never been identified by experimental methods and we aimed to characterize it in the present study. |
| T6 | 678-853 | Sentence | denotes | To achieve this, the two genes, mnp1 and mnp2, were cloned and the gene products were overexpressed, purified, and analyzed in vitro for their respective enzymatic activities. |
| T7 | 854-1052 | Sentence | denotes | Products of reactions catalyzed by Mnp1 and Mnp2 were detected by capillary electrophoresis and validated using electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. |
| T8 | 1053-1253 | Sentence | denotes | We show that Mnp1 is responsible for the transfer of CMP from CTP to d-fructose-6-phosphate (Fru-6-P) to form CDP-d-fructose, whereas Mnp2 catalyzed the conversion of CDP-d-fructose to CDP-d-mannitol. |
| T9 | 1254-1421 | Sentence | denotes | Therefore, Mnp1 (renamed as mnpA) was identified as Fru-6-P cytidylyltransferase-encoding gene, and mnp2 (renamed as mnpB) as a CDP-d-fructose reductase-encoding gene. |
| T10 | 1422-1591 | Sentence | denotes | The kinetics of Mnp1 for the substrate (Fru-6-P and CTP) and of Mnp2 for the substrate (CDP-d-fructose) and the cofactor NADH or NADPH fitted the Michaelis-Menten model. |
| T11 | 1592-1668 | Sentence | denotes | The effects of temperature, pH and cations on the two enzymes were analyzed. |
| T12 | 1669-1774 | Sentence | denotes | This is the first time that the biosynthetic pathway of CDP-d-mannitol has been identified biochemically. |
| T1 | 0-92 | Sentence | denotes | Characterization of the CDP-D-mannitol biosynthetic pathway in Streptococcus pneumoniae 35A. |
| T2 | 93-179 | Sentence | denotes | Streptococcus pneumoniae is a major human pathogen associated with diseases worldwide. |
| T3 | 180-368 | Sentence | denotes | The capsular polysaccharides (CPSs) are considered a major virulence factor and are targets for a vaccine. d-Mannitol was found to be present in the CPS of several S. pneumoniae serotypes. |
| T4 | 369-552 | Sentence | denotes | Two genes, mnp1 and mnp2, which are located in the CPS gene cluster, were proposed to be responsible for the synthesis of NDP-d-mannitol (the nucleotide activated form of d-mannitol). |
| T5 | 553-677 | Sentence | denotes | However, the pathway has never been identified by experimental methods and we aimed to characterize it in the present study. |
| T6 | 678-853 | Sentence | denotes | To achieve this, the two genes, mnp1 and mnp2, were cloned and the gene products were overexpressed, purified, and analyzed in vitro for their respective enzymatic activities. |
| T7 | 854-1052 | Sentence | denotes | Products of reactions catalyzed by Mnp1 and Mnp2 were detected by capillary electrophoresis and validated using electrospray ionization mass spectrometry and nuclear magnetic resonance spectroscopy. |
| T8 | 1053-1253 | Sentence | denotes | We show that Mnp1 is responsible for the transfer of CMP from CTP to d-fructose-6-phosphate (Fru-6-P) to form CDP-d-fructose, whereas Mnp2 catalyzed the conversion of CDP-d-fructose to CDP-d-mannitol. |
| T9 | 1254-1421 | Sentence | denotes | Therefore, Mnp1 (renamed as mnpA) was identified as Fru-6-P cytidylyltransferase-encoding gene, and mnp2 (renamed as mnpB) as a CDP-d-fructose reductase-encoding gene. |
| T10 | 1422-1591 | Sentence | denotes | The kinetics of Mnp1 for the substrate (Fru-6-P and CTP) and of Mnp2 for the substrate (CDP-d-fructose) and the cofactor NADH or NADPH fitted the Michaelis-Menten model. |
| T11 | 1592-1668 | Sentence | denotes | The effects of temperature, pH and cations on the two enzymes were analyzed. |
| T12 | 1669-1774 | Sentence | denotes | This is the first time that the biosynthetic pathway of CDP-d-mannitol has been identified biochemically. |
GlycoBiology-PACDB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 63-76 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC725 | denotes | Streptococcus |
| _T2 | 63-87 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC530 | denotes | Streptococcus pneumoniae |
| _T3 | 63-87 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC672 | denotes | Streptococcus pneumoniae |
| _T4 | 63-87 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC115,LEC118,LEC374,LEC408,LEC423,LEC435,LEC440,LEC557,LEC639 | denotes | Streptococcus pneumoniae |
| _T5 | 63-87 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC707 | denotes | Streptococcus pneumoniae |
| _T6 | 63-87 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC061 | denotes | Streptococcus pneumoniae |
| _T7 | 63-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC729 | denotes | Streptococcus pneumoniae 35A. Streptococcus pneumoniae |
| _T8 | 63-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC457 | denotes | Streptococcus pneumoniae 35A. Streptococcus pneumoniae |
| _T9 | 93-106 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC725 | denotes | Streptococcus |
| _T10 | 93-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC530 | denotes | Streptococcus pneumoniae |
| _T11 | 93-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC707 | denotes | Streptococcus pneumoniae |
| _T12 | 93-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC061 | denotes | Streptococcus pneumoniae |
| _T13 | 93-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC115,LEC118,LEC374,LEC408,LEC423,LEC435,LEC440,LEC557,LEC639 | denotes | Streptococcus pneumoniae |
| _T14 | 93-117 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC672 | denotes | Streptococcus pneumoniae |
ICD10
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 77-87 | http://purl.bioontology.org/ontology/ICD10/J18.9 | denotes | pneumoniae |
| T2 | 107-117 | http://purl.bioontology.org/ontology/ICD10/J18.9 | denotes | pneumoniae |
| T3 | 347-357 | http://purl.bioontology.org/ontology/ICD10/J18.9 | denotes | pneumoniae |
GlycoBiology-FMA
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 193-208 | FMAID:82746 | denotes | polysaccharides |
| _T2 | 193-208 | FMAID:196735 | denotes | polysaccharides |
| _T3 | 193-208 | FMAID:82785 | denotes | polysaccharides |
| _T4 | 193-208 | FMAID:196779 | denotes | polysaccharides |
| _T5 | 373-378 | FMAID:198663 | denotes | genes |
| _T6 | 424-428 | FMAID:198663 | denotes | gene |
| _T7 | 424-436 | FMAID:198017 | denotes | gene cluster |
| _T8 | 424-436 | FMAID:84082 | denotes | gene cluster |
| _T9 | 511-521 | FMAID:196729 | denotes | nucleotide |
| _T10 | 511-521 | FMAID:82740 | denotes | nucleotide |
| _T11 | 703-708 | FMAID:198663 | denotes | genes |
| _T12 | 745-749 | FMAID:198663 | denotes | gene |
| _T13 | 920-929 | FMAID:167591 | denotes | capillary |
| _T14 | 920-929 | FMAID:63194 | denotes | capillary |
| _T15 | 1344-1348 | FMAID:198663 | denotes | gene |
| _T16 | 1416-1420 | FMAID:198663 | denotes | gene |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 24-27 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T2 | 1163-1166 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T3 | 1220-1223 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T4 | 1238-1241 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T5 | 1382-1385 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T6 | 1510-1513 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T7 | 1725-1728 | http://www.uniprot.org/uniprot/P01160 | denotes | CDP |
| T8 | 24-27 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T9 | 1163-1166 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T10 | 1220-1223 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T11 | 1238-1241 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T12 | 1382-1385 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T13 | 1510-1513 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T14 | 1725-1728 | http://www.uniprot.org/uniprot/Q9UEV5 | denotes | CDP |
| T15 | 491-494 | http://www.uniprot.org/uniprot/Q00604 | denotes | NDP |
| T16 | 1106-1109 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
| T17 | 1115-1118 | http://www.uniprot.org/uniprot/P53007 | denotes | CTP |
| T18 | 1474-1477 | http://www.uniprot.org/uniprot/P53007 | denotes | CTP |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 24-27 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T2 | 1163-1166 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T3 | 1220-1223 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T4 | 1238-1241 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T5 | 1382-1385 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T6 | 1510-1513 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
| T7 | 1725-1728 | http://www.uniprot.org/uniprot/P53564 | denotes | CDP |
GlycoBiology-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 63-76 | http://purl.bioontology.org/ontology/NCBITAXON/155680 | denotes | Streptococcus |
| T2 | 63-76 | http://purl.bioontology.org/ontology/NCBITAXON/1306 | denotes | Streptococcus |
| T3 | 63-76 | http://purl.bioontology.org/ontology/NCBITAXON/1301 | denotes | Streptococcus |
| T4 | 63-87 | http://purl.bioontology.org/ontology/NCBITAXON/257758 | denotes | Streptococcus pneumoniae |
| T5 | 63-87 | http://purl.bioontology.org/ontology/NCBITAXON/1313 | denotes | Streptococcus pneumoniae |
| T6 | 93-106 | http://purl.bioontology.org/ontology/NCBITAXON/155680 | denotes | Streptococcus |
| T7 | 93-106 | http://purl.bioontology.org/ontology/NCBITAXON/1301 | denotes | Streptococcus |
| T8 | 93-106 | http://purl.bioontology.org/ontology/NCBITAXON/1306 | denotes | Streptococcus |
| T9 | 93-117 | http://purl.bioontology.org/ontology/NCBITAXON/1313 | denotes | Streptococcus pneumoniae |
| T10 | 93-117 | http://purl.bioontology.org/ontology/NCBITAXON/257758 | denotes | Streptococcus pneumoniae |
| T11 | 578-583 | http://purl.bioontology.org/ontology/NCBITAXON/617048 | denotes | never |
| T12 | 920-929 | http://purl.bioontology.org/ontology/NCBITAXON/241203 | denotes | capillary |
| T13 | 920-929 | http://purl.bioontology.org/ontology/NCBITAXON/119095 | denotes | capillary |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 239-248 | http://purl.obolibrary.org/obo/GO_0009405 | denotes | virulence |
| T2 | 239-248 | http://purl.obolibrary.org/obo/GO_0016032 | denotes | virulence |
| T3 | 478-487 | http://purl.obolibrary.org/obo/GO_0009058 | denotes | synthesis |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 920-929 | http://purl.obolibrary.org/obo/UBERON_0001982 | denotes | capillary |
Allie
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| SS1_22833313_2_0 | 184-208 | expanded | denotes | capsular polysaccharides |
| SS2_22833313_2_0 | 210-214 | abbr | denotes | CPSs |
| SS1_22833313_7_0 | 1122-1144 | expanded | denotes | d-fructose-6-phosphate |
| SS2_22833313_7_0 | 1146-1153 | abbr | denotes | Fru-6-P |
| AE1_22833313_2_0 | SS1_22833313_2_0 | SS2_22833313_2_0 | abbreviatedTo | capsular polysaccharides,CPSs |
| AE1_22833313_7_0 | SS1_22833313_7_0 | SS2_22833313_7_0 | abbreviatedTo | d-fructose-6-phosphate,Fru-6-P |
GlyTouCan-IUPAC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| GlycanIUPAC_T1 | 1146-1149 | "http://rdf.glycoinfo.org/glycan/G24678II" | denotes | Fru |
| GlycanIUPAC_T2 | 1306-1309 | "http://rdf.glycoinfo.org/glycan/G24678II" | denotes | Fru |
| GlycanIUPAC_T3 | 1462-1465 | "http://rdf.glycoinfo.org/glycan/G24678II" | denotes | Fru |
| GlycanIUPAC_T4 | 1146-1149 | "http://rdf.glycoinfo.org/glycan/G52698BL" | denotes | Fru |
| GlycanIUPAC_T5 | 1306-1309 | "http://rdf.glycoinfo.org/glycan/G52698BL" | denotes | Fru |
| GlycanIUPAC_T6 | 1462-1465 | "http://rdf.glycoinfo.org/glycan/G52698BL" | denotes | Fru |
performance-test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-UBERON-AE-B_T1 | 920-929 | http://purl.obolibrary.org/obo/UBERON_0001982 | denotes | capillary |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 24-29 | Disease | denotes | CDP-D | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T2 | 63-87 | Disease | denotes | Streptococcus pneumoniae | http://purl.obolibrary.org/obo/MONDO_0005972 |
| T3 | 93-117 | Disease | denotes | Streptococcus pneumoniae | http://purl.obolibrary.org/obo/MONDO_0005972 |
| T4 | 347-357 | Disease | denotes | pneumoniae | http://purl.obolibrary.org/obo/MONDO_0005249 |
| T5 | 491-494 | Disease | denotes | NDP | http://purl.obolibrary.org/obo/MONDO_0010691 |
| T6 | 1163-1168 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T7 | 1220-1225 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T8 | 1238-1243 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T9 | 1382-1387 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T10 | 1510-1515 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
| T11 | 1725-1730 | Disease | denotes | CDP-d | http://purl.obolibrary.org/obo/MONDO_0009015 |
HP-phenotype
| Id | Subject | Object | Predicate | Lexical cue | hp_id |
|---|---|---|---|---|---|
| T1 | 77-87 | Phenotype | denotes | pneumoniae | HP:0002090 |
| T2 | 107-117 | Phenotype | denotes | pneumoniae | HP:0002090 |
| T3 | 347-357 | Phenotype | denotes | pneumoniae | HP:0002090 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 63-87 | OrganismTaxon | denotes | Streptococcus pneumoniae | 1313 |
| T2 | 93-117 | OrganismTaxon | denotes | Streptococcus pneumoniae | 1313 |
| T3 | 129-134 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 920-929 | Body_part | denotes | capillary | http://purl.obolibrary.org/obo/UBERON_0001982 |