| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-118 |
Sentence |
denotes |
MicroRNA-199a targets CD44 to suppress the tumorigenicity and multidrug resistance of ovarian cancer-initiating cells. |
| TextSentencer_T2 |
119-317 |
Sentence |
denotes |
In ovarian cancer, CD44(+) /CD117(+) stem cells, also known as cancer-initiating cells (CICs), are highly proliferative, have a low degree of differentiation, and are resistant to chemotherapeutics. |
| TextSentencer_T3 |
318-435 |
Sentence |
denotes |
Therefore, the CD44(+) /CD117(+) subpopulation is thought to be an important target for novel therapeutic strategies. |
| TextSentencer_T4 |
436-533 |
Sentence |
denotes |
In this study, we investigated the role of microRNA-199a (miR-199a) in ovarian cancer stem cells. |
| TextSentencer_T5 |
534-646 |
Sentence |
denotes |
Luciferase reporter gene assays confirmed that miR-199a targets CD44 via an miR-199a-binding site in the 3'-UTR. |
| TextSentencer_T6 |
647-829 |
Sentence |
denotes |
CD44(+) /CD117(+) ovarian CICs were enriched from human primary ovarian tumor tissues and confirmed by flow cytometric sorting. miR-199a was cloned and transfected into ovarian CICs. |
| TextSentencer_T7 |
830-997 |
Sentence |
denotes |
CD44 mRNA and protein expression was significantly decreased in miR-199a-transfected ovarian CICs as compared with miR-199a mutant-transfected and untransfected cells. |
| TextSentencer_T8 |
998-1561 |
Sentence |
denotes |
Cell cycle analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide proliferation assays, the colony formation assay and the transwell migration assay indicated that miR-199a significantly affected cell cycle regulation and suppressed the proliferation and invasive capacity of ovarian CICs in vitro. miR-199a significantly increased the chemosensitivity of ovarian CICs to cisplatin, pacitaxel, and adriamycin, and reduced mRNA expression of the multidrug resistance gene ABCG2 as compared with miR-199a mutant-transfected and untransfected cells. |
| TextSentencer_T9 |
1562-1734 |
Sentence |
denotes |
The expression of stemness markers was also significantly reduced in miR-199a-transfected CICs as compared with miR-199a mutant-transfected and untransfected ovarian cells. |
| TextSentencer_T10 |
1735-1867 |
Sentence |
denotes |
Furthermore, xenograft experiments confirmed that miR-199a suppressed the growth of xenograft tumors formed by ovarian CICs in vivo. |
| TextSentencer_T11 |
1868-2014 |
Sentence |
denotes |
Thus, expression of endogenous mature miR-199a may prevent tumorigenesis in human ovarian cancer by regulating expression of its target gene CD44. |
| T1 |
0-118 |
Sentence |
denotes |
MicroRNA-199a targets CD44 to suppress the tumorigenicity and multidrug resistance of ovarian cancer-initiating cells. |
| T2 |
119-317 |
Sentence |
denotes |
In ovarian cancer, CD44(+) /CD117(+) stem cells, also known as cancer-initiating cells (CICs), are highly proliferative, have a low degree of differentiation, and are resistant to chemotherapeutics. |
| T3 |
318-435 |
Sentence |
denotes |
Therefore, the CD44(+) /CD117(+) subpopulation is thought to be an important target for novel therapeutic strategies. |
| T4 |
436-533 |
Sentence |
denotes |
In this study, we investigated the role of microRNA-199a (miR-199a) in ovarian cancer stem cells. |
| T5 |
534-646 |
Sentence |
denotes |
Luciferase reporter gene assays confirmed that miR-199a targets CD44 via an miR-199a-binding site in the 3'-UTR. |
| T6 |
647-829 |
Sentence |
denotes |
CD44(+) /CD117(+) ovarian CICs were enriched from human primary ovarian tumor tissues and confirmed by flow cytometric sorting. miR-199a was cloned and transfected into ovarian CICs. |
| T7 |
830-997 |
Sentence |
denotes |
CD44 mRNA and protein expression was significantly decreased in miR-199a-transfected ovarian CICs as compared with miR-199a mutant-transfected and untransfected cells. |
| T8 |
998-1561 |
Sentence |
denotes |
Cell cycle analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide proliferation assays, the colony formation assay and the transwell migration assay indicated that miR-199a significantly affected cell cycle regulation and suppressed the proliferation and invasive capacity of ovarian CICs in vitro. miR-199a significantly increased the chemosensitivity of ovarian CICs to cisplatin, pacitaxel, and adriamycin, and reduced mRNA expression of the multidrug resistance gene ABCG2 as compared with miR-199a mutant-transfected and untransfected cells. |
| T9 |
1562-1734 |
Sentence |
denotes |
The expression of stemness markers was also significantly reduced in miR-199a-transfected CICs as compared with miR-199a mutant-transfected and untransfected ovarian cells. |
| T10 |
1735-1867 |
Sentence |
denotes |
Furthermore, xenograft experiments confirmed that miR-199a suppressed the growth of xenograft tumors formed by ovarian CICs in vivo. |
| T11 |
1868-2014 |
Sentence |
denotes |
Thus, expression of endogenous mature miR-199a may prevent tumorigenesis in human ovarian cancer by regulating expression of its target gene CD44. |