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PubMed:22434778 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 970-981 https://glytoucan.org/Structures/Glycans/G25326UC denotes xyloglucans
T2 1153-1160 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 970-981 Glycan_Motif denotes xyloglucans https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G25326UC
T2 1153-1160 Glycan_Motif denotes glucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 970-981 https://glytoucan.org/Structures/Glycans/G25326UC denotes xyloglucans
T2 1153-1160 https://glytoucan.org/Structures/Glycans/G15021LG denotes glucose

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 21-33 FMAID:197276 denotes carbohydrate
_T2 21-33 FMAID:82737 denotes carbohydrate
_T3 98-110 FMAID:197276 denotes carbohydrate
_T4 98-110 FMAID:82737 denotes carbohydrate
_T5 174-187 FMAID:197276 denotes carbohydrates
_T6 174-187 FMAID:82737 denotes carbohydrates
_T7 258-270 FMAID:197276 denotes carbohydrate
_T8 258-270 FMAID:82737 denotes carbohydrate
_T9 281-293 FMAID:197276 denotes Carbohydrate
_T10 281-293 FMAID:82737 denotes Carbohydrate
_T11 339-351 FMAID:197276 denotes carbohydrate
_T12 339-351 FMAID:82737 denotes carbohydrate
_T13 439-452 FMAID:197276 denotes carbohydrates
_T14 439-452 FMAID:82737 denotes carbohydrates
_T15 593-605 FMAID:197276 denotes carbohydrate
_T16 593-605 FMAID:82737 denotes carbohydrate
_T17 606-613 FMAID:165447 denotes protein
_T18 606-613 FMAID:67257 denotes protein
_T19 1114-1120 FMAID:82804 denotes xylose
_T20 1114-1120 FMAID:196799 denotes xylose
_T21 1153-1160 FMAID:82743 denotes glucose
_T22 1153-1160 FMAID:196732 denotes glucose
_T23 1201-1208 FMAID:82757 denotes leucine
_T24 1201-1208 FMAID:196746 denotes leucine
_T25 1212-1225 FMAID:196743 denotes phenylalanine
_T26 1212-1225 FMAID:82754 denotes phenylalanine
_T27 1315-1316 FMAID:214745 denotes π
_T28 1472-1479 FMAID:165447 denotes protein
_T29 1472-1479 FMAID:67257 denotes protein
_T30 1680-1687 FMAID:67257 denotes protein
_T31 1680-1687 FMAID:165447 denotes protein
_T32 1688-1700 FMAID:197276 denotes carbohydrate
_T33 1688-1700 FMAID:82737 denotes carbohydrate
_T34 1767-1779 FMAID:197276 denotes carbohydrate
_T35 1767-1779 FMAID:82737 denotes carbohydrate

uniprot-human

Id Subject Object Predicate Lexical cue
T1 1073-1076 http://www.uniprot.org/uniprot/P08519 denotes apo

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 1315-1316 http://www.uniprot.org/uniprot/Q50H32 denotes π

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 826-833 http://purl.bioontology.org/ontology/NCBITAXON/353209 denotes related
T2 1567-1572 http://purl.bioontology.org/ontology/STY/T096 denotes group

GO-MF

Id Subject Object Predicate Lexical cue
T1 21-41 http://purl.obolibrary.org/obo/GO_0030246 denotes carbohydrate-binding
T2 98-118 http://purl.obolibrary.org/obo/GO_0030246 denotes carbohydrate-binding
T3 281-301 http://purl.obolibrary.org/obo/GO_0030246 denotes Carbohydrate-binding
T4 34-41 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T5 111-118 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T6 294-301 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T7 742-749 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T8 865-872 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T9 241-245 http://purl.obolibrary.org/obo/GO_0070026 denotes bind
T10 398-402 http://purl.obolibrary.org/obo/GO_0070026 denotes bind
T11 964-969 http://purl.obolibrary.org/obo/GO_0070026 denotes binds
T12 34-41 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T13 111-118 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T14 294-301 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T15 742-749 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T16 865-872 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T17 241-245 http://purl.obolibrary.org/obo/GO_0003680 denotes bind
T18 398-402 http://purl.obolibrary.org/obo/GO_0003680 denotes bind
T19 964-969 http://purl.obolibrary.org/obo/GO_0003680 denotes binds
T20 34-41 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T21 111-118 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T22 294-301 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T23 742-749 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T24 865-872 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T25 241-245 http://purl.obolibrary.org/obo/GO_0017091 denotes bind
T26 398-402 http://purl.obolibrary.org/obo/GO_0017091 denotes bind
T27 964-969 http://purl.obolibrary.org/obo/GO_0017091 denotes binds
T28 34-41 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T29 111-118 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T30 294-301 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T31 742-749 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T32 865-872 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T33 241-245 http://purl.obolibrary.org/obo/GO_0005488 denotes bind
T34 398-402 http://purl.obolibrary.org/obo/GO_0005488 denotes bind
T35 964-969 http://purl.obolibrary.org/obo/GO_0005488 denotes binds
T36 1375-1382 http://purl.obolibrary.org/obo/GO_0005488 denotes ligands

Allie

Id Subject Object Predicate Lexical cue
SS1_22434778_2_0 281-309 expanded denotes Carbohydrate-binding modules
SS2_22434778_2_0 311-315 abbr denotes CBMs
AE1_22434778_2_0 SS1_22434778_2_0 SS2_22434778_2_0 abbreviatedTo Carbohydrate-binding modules,CBMs

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-127 Sentence denotes Structural basis for carbohydrate-binding specificity--a comparative assessment of two engineered carbohydrate-binding modules.
TextSentencer_T2 128-280 Sentence denotes Detection, immobilization and purification of carbohydrates can be done using molecular probes that specifically bind to targeted carbohydrate epitopes.
TextSentencer_T3 281-453 Sentence denotes Carbohydrate-binding modules (CBMs) are discrete parts of carbohydrate-hydrolyzing enzymes that can be engineered to bind and detect specifically a number of carbohydrates.
TextSentencer_T4 454-627 Sentence denotes Design and engineering of CBMs have benefited greatly from structural studies that have helped us to decipher the basis for specificity in carbohydrate-protein interactions.
TextSentencer_T5 628-761 Sentence denotes However, more studies are needed to predict which modifications in a CBM would generate probes with predetermined binding properties.
TextSentencer_T6 762-1018 Sentence denotes In this report, we present the crystal structures of two highly related engineered CBMs with different binding specificity profiles: X-2, which is specific for xylans and the L110F mutant of X-2, which binds xyloglucans and β-glucans in addition to xylans.
TextSentencer_T7 1019-1186 Sentence denotes The structures of the modules were solved both in the apo form and complexed with oligomers of xylose, as well as with an oligomer of glucose in the case of X-2 L110F.
TextSentencer_T8 1187-1383 Sentence denotes The mutation, leucine to phenylalanine, converting the specific module into a cross-reactive one, introduces a crucial hydrogen-π interaction that allows the mutant to retain glucan-based ligands.
TextSentencer_T9 1384-1619 Sentence denotes The cross-reactivity of X-2 L110F is furthermore made possible by the plasticity of the protein, in particular, of residue R142, which permits accommodation of an extra hydroxymethyl group present in cellopentaose and not xylopentaose.
TextSentencer_T10 1620-1832 Sentence denotes Altogether, this study shows, in structural detail, altered protein-carbohydrate interactions that have high impact on the binding properties of a carbohydrate probe but are introduced through simple mutagenesis.
T1 0-127 Sentence denotes Structural basis for carbohydrate-binding specificity--a comparative assessment of two engineered carbohydrate-binding modules.
T2 128-280 Sentence denotes Detection, immobilization and purification of carbohydrates can be done using molecular probes that specifically bind to targeted carbohydrate epitopes.
T3 281-453 Sentence denotes Carbohydrate-binding modules (CBMs) are discrete parts of carbohydrate-hydrolyzing enzymes that can be engineered to bind and detect specifically a number of carbohydrates.
T4 454-627 Sentence denotes Design and engineering of CBMs have benefited greatly from structural studies that have helped us to decipher the basis for specificity in carbohydrate-protein interactions.
T5 628-761 Sentence denotes However, more studies are needed to predict which modifications in a CBM would generate probes with predetermined binding properties.
T6 762-1018 Sentence denotes In this report, we present the crystal structures of two highly related engineered CBMs with different binding specificity profiles: X-2, which is specific for xylans and the L110F mutant of X-2, which binds xyloglucans and β-glucans in addition to xylans.
T7 1019-1186 Sentence denotes The structures of the modules were solved both in the apo form and complexed with oligomers of xylose, as well as with an oligomer of glucose in the case of X-2 L110F.
T8 1187-1383 Sentence denotes The mutation, leucine to phenylalanine, converting the specific module into a cross-reactive one, introduces a crucial hydrogen-π interaction that allows the mutant to retain glucan-based ligands.
T9 1384-1619 Sentence denotes The cross-reactivity of X-2 L110F is furthermore made possible by the plasticity of the protein, in particular, of residue R142, which permits accommodation of an extra hydroxymethyl group present in cellopentaose and not xylopentaose.
T10 1620-1832 Sentence denotes Altogether, this study shows, in structural detail, altered protein-carbohydrate interactions that have high impact on the binding properties of a carbohydrate probe but are introduced through simple mutagenesis.
T1 0-127 Sentence denotes Structural basis for carbohydrate-binding specificity--a comparative assessment of two engineered carbohydrate-binding modules.
T2 128-280 Sentence denotes Detection, immobilization and purification of carbohydrates can be done using molecular probes that specifically bind to targeted carbohydrate epitopes.
T3 281-453 Sentence denotes Carbohydrate-binding modules (CBMs) are discrete parts of carbohydrate-hydrolyzing enzymes that can be engineered to bind and detect specifically a number of carbohydrates.
T4 454-627 Sentence denotes Design and engineering of CBMs have benefited greatly from structural studies that have helped us to decipher the basis for specificity in carbohydrate-protein interactions.
T5 628-761 Sentence denotes However, more studies are needed to predict which modifications in a CBM would generate probes with predetermined binding properties.
T6 762-1018 Sentence denotes In this report, we present the crystal structures of two highly related engineered CBMs with different binding specificity profiles: X-2, which is specific for xylans and the L110F mutant of X-2, which binds xyloglucans and β-glucans in addition to xylans.
T7 1019-1186 Sentence denotes The structures of the modules were solved both in the apo form and complexed with oligomers of xylose, as well as with an oligomer of glucose in the case of X-2 L110F.
T8 1187-1383 Sentence denotes The mutation, leucine to phenylalanine, converting the specific module into a cross-reactive one, introduces a crucial hydrogen-π interaction that allows the mutant to retain glucan-based ligands.
T9 1384-1619 Sentence denotes The cross-reactivity of X-2 L110F is furthermore made possible by the plasticity of the protein, in particular, of residue R142, which permits accommodation of an extra hydroxymethyl group present in cellopentaose and not xylopentaose.
T10 1620-1832 Sentence denotes Altogether, this study shows, in structural detail, altered protein-carbohydrate interactions that have high impact on the binding properties of a carbohydrate probe but are introduced through simple mutagenesis.

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 970-981 http://www.glycoepitope.jp/epitopes/EP0511 denotes xyloglucans

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 970-981 Glycan denotes xyloglucans https://api.glycosmos.org/wurcs2image/latest/png/binary/G25326UC

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-127 Sentence denotes Structural basis for carbohydrate-binding specificity--a comparative assessment of two engineered carbohydrate-binding modules.
T2 128-280 Sentence denotes Detection, immobilization and purification of carbohydrates can be done using molecular probes that specifically bind to targeted carbohydrate epitopes.
T3 281-453 Sentence denotes Carbohydrate-binding modules (CBMs) are discrete parts of carbohydrate-hydrolyzing enzymes that can be engineered to bind and detect specifically a number of carbohydrates.
T4 454-627 Sentence denotes Design and engineering of CBMs have benefited greatly from structural studies that have helped us to decipher the basis for specificity in carbohydrate-protein interactions.
T5 628-761 Sentence denotes However, more studies are needed to predict which modifications in a CBM would generate probes with predetermined binding properties.
T6 762-1018 Sentence denotes In this report, we present the crystal structures of two highly related engineered CBMs with different binding specificity profiles: X-2, which is specific for xylans and the L110F mutant of X-2, which binds xyloglucans and β-glucans in addition to xylans.
T7 1019-1186 Sentence denotes The structures of the modules were solved both in the apo form and complexed with oligomers of xylose, as well as with an oligomer of glucose in the case of X-2 L110F.
T8 1187-1383 Sentence denotes The mutation, leucine to phenylalanine, converting the specific module into a cross-reactive one, introduces a crucial hydrogen-π interaction that allows the mutant to retain glucan-based ligands.
T9 1384-1619 Sentence denotes The cross-reactivity of X-2 L110F is furthermore made possible by the plasticity of the protein, in particular, of residue R142, which permits accommodation of an extra hydroxymethyl group present in cellopentaose and not xylopentaose.
T10 1620-1832 Sentence denotes Altogether, this study shows, in structural detail, altered protein-carbohydrate interactions that have high impact on the binding properties of a carbohydrate probe but are introduced through simple mutagenesis.