PubMed:22355346 JSONTXT

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":881,"end":886},"obj":"gene:124"},{"id":"T1","span":{"begin":830,"end":832},"obj":"disease:C0700095"},{"id":"T2","span":{"begin":881,"end":886},"obj":"gene:124"},{"id":"T3","span":{"begin":830,"end":832},"obj":"disease:C0027819"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"N-cadherin in neuroblastoma disease: expression and clinical significance.\nOne of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability."}

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":329,"end":342},"obj":"HP_0003006"},{"id":"T2","span":{"begin":357,"end":381},"obj":"HP_0030061"},{"id":"T3","span":{"begin":357,"end":362},"obj":"HP_0002664"},{"id":"T4","span":{"begin":556,"end":569},"obj":"HP_0003006"},{"id":"T5","span":{"begin":556,"end":576},"obj":"HP_0004376"},{"id":"T6","span":{"begin":570,"end":576},"obj":"HP_0002664"},{"id":"T7","span":{"begin":597,"end":609},"obj":"HP_0003006"},{"id":"T8","span":{"begin":597,"end":616},"obj":"HP_0004376"},{"id":"T9","span":{"begin":610,"end":616},"obj":"HP_0002664"},{"id":"T10","span":{"begin":759,"end":764},"obj":"HP_0002664"},{"id":"T11","span":{"begin":944,"end":949},"obj":"HP_0002664"},{"id":"T12","span":{"begin":1066,"end":1079},"obj":"HP_0003006"},{"id":"T13","span":{"begin":1139,"end":1152},"obj":"HP_0003006"}],"text":"N-cadherin in neuroblastoma disease: expression and clinical significance.\nOne of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability."}

{"project":"Allie","denotations":[{"id":"SS1_22355346_2_0","span":{"begin":329,"end":342},"obj":"expanded"},{"id":"SS2_22355346_2_0","span":{"begin":344,"end":346},"obj":"abbr"},{"id":"SS1_22355346_3_0","span":{"begin":396,"end":434},"obj":"expanded"},{"id":"SS2_22355346_3_0","span":{"begin":436,"end":443},"obj":"abbr"},{"id":"SS1_22355346_3_1","span":{"begin":484,"end":502},"obj":"expanded"},{"id":"SS2_22355346_3_1","span":{"begin":504,"end":507},"obj":"abbr"}],"relations":[{"id":"AE1_22355346_2_0","pred":"abbreviatedTo","subj":"SS1_22355346_2_0","obj":"SS2_22355346_2_0"},{"id":"AE1_22355346_3_0","pred":"abbreviatedTo","subj":"SS1_22355346_3_0","obj":"SS2_22355346_3_0"},{"id":"AE1_22355346_3_1","pred":"abbreviatedTo","subj":"SS1_22355346_3_1","obj":"SS2_22355346_3_1"}],"text":"N-cadherin in neuroblastoma disease: expression and clinical significance.\nOne of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"22355346-6#64#69#gene124","span":{"begin":881,"end":886},"obj":"gene124"},{"id":"22355346-6#13#15#diseaseC0700095","span":{"begin":830,"end":832},"obj":"diseaseC0700095"},{"id":"22355346-6#13#15#diseaseC0027819","span":{"begin":830,"end":832},"obj":"diseaseC0027819"}],"relations":[{"id":"64#69#gene12413#15#diseaseC0700095","pred":"associated_with","subj":"22355346-6#64#69#gene124","obj":"22355346-6#13#15#diseaseC0700095"},{"id":"64#69#gene12413#15#diseaseC0027819","pred":"associated_with","subj":"22355346-6#64#69#gene124","obj":"22355346-6#13#15#diseaseC0027819"}],"text":"N-cadherin in neuroblastoma disease: expression and clinical significance.\nOne of the first and most important steps in the metastatic cascade is the loss of cell-cell and cell-matrix interactions. N-cadherin, a crucial mediator of homotypic and heterotypic cell-cell interactions, might play a central role in the metastasis of neuroblastoma (NB), a solid tumor of neuroectodermal origin. Using Reverse Transcription Quantitative PCR (RT-qPCR), Western blot, immunocytochemistry and Tissue MicroArrays (TMA) we demonstrate the expression of N-cadherin in neuroblastoma tumors and cell lines. All neuroblastic tumors (n = 356) and cell lines (n = 10) expressed various levels of the adhesion protein. The N-cadherin mRNA expression was significantly lower in tumor samples from patients suffering metastatic disease. Treatment of NB cell lines with the N-cadherin blocking peptide ADH-1 (Exherin, Adherex Technologies Inc.), strongly inhibited tumor cell proliferation in vitro by inducing apoptosis. Our results suggest that N-cadherin signaling may play a role in neuroblastoma disease, marking involvement of metastasis and determining neuroblastoma cell viability."}