PubMed:22303963 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_22303963_1_0","span":{"begin":302,"end":340},"obj":"expanded"},{"id":"SS2_22303963_1_0","span":{"begin":342,"end":346},"obj":"abbr"},{"id":"SS1_22303963_1_1","span":{"begin":353,"end":370},"obj":"expanded"},{"id":"SS2_22303963_1_1","span":{"begin":372,"end":374},"obj":"abbr"}],"relations":[{"id":"AE1_22303963_1_0","pred":"abbreviatedTo","subj":"SS1_22303963_1_0","obj":"SS2_22303963_1_0"},{"id":"AE1_22303963_1_1","pred":"abbreviatedTo","subj":"SS1_22303963_1_1","obj":"SS2_22303963_1_1"}],"text":"Determination of three steroidal saponins from Ophiopogon japonicus (Liliaceae) via high-performance liquid chromatography with mass spectrometry.\nA simple and accurate analytical method was developed for simultaneous quantification of three steroidal saponins in the roots of Ophiopogon japonicus via high-performance liquid chromatography (HPLC) with mass spectrometry (MS) in this study. Separation was performed on a Tigerkin C(18) column and detection was performed by mass spectrometry. A mobile phase consisted of 0.02% formic acid in water (v/v) and 0.02% formic acid in acetonitrile (v/v) was used with a flow rate of 0.5 mL min(-1). The quantitative HPLC-MS method was validated for linearity, precision, repeatability, stability, recovery, limits of detection and quantification. This developed method provides good linearity (r \u003e0.9993), intra- and inter-day precisions (RSD \u003c4.18%), repeatability (RSD \u003c5.05%), stability (RSD \u003c2.08%) and recovery (93.82-102.84%) for three steroidal saponins. It could be considered as a suitable quality control method for O. japonicus."}

{"project":"CHEMDNER-training-test","denotations":[{"id":"T1","span":{"begin":242,"end":260},"obj":"FAMILY"},{"id":"T2","span":{"begin":527,"end":538},"obj":"SYSTEMATIC"},{"id":"T3","span":{"begin":564,"end":575},"obj":"SYSTEMATIC"},{"id":"T4","span":{"begin":579,"end":591},"obj":"SYSTEMATIC"},{"id":"T5","span":{"begin":986,"end":1004},"obj":"FAMILY"},{"id":"T1","span":{"begin":23,"end":41},"obj":"FAMILY"}],"text":"Determination of three steroidal saponins from Ophiopogon japonicus (Liliaceae) via high-performance liquid chromatography with mass spectrometry.\nA simple and accurate analytical method was developed for simultaneous quantification of three steroidal saponins in the roots of Ophiopogon japonicus via high-performance liquid chromatography (HPLC) with mass spectrometry (MS) in this study. Separation was performed on a Tigerkin C(18) column and detection was performed by mass spectrometry. A mobile phase consisted of 0.02% formic acid in water (v/v) and 0.02% formic acid in acetonitrile (v/v) was used with a flow rate of 0.5 mL min(-1). The quantitative HPLC-MS method was validated for linearity, precision, repeatability, stability, recovery, limits of detection and quantification. This developed method provides good linearity (r \u003e0.9993), intra- and inter-day precisions (RSD \u003c4.18%), repeatability (RSD \u003c5.05%), stability (RSD \u003c2.08%) and recovery (93.82-102.84%) for three steroidal saponins. It could be considered as a suitable quality control method for O. japonicus."}