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PubMed:22042767 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
TextSentencer_T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
TextSentencer_T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
TextSentencer_T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
TextSentencer_T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
TextSentencer_T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
TextSentencer_T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
TextSentencer_T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
TextSentencer_T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
TextSentencer_T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
TextSentencer_T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.
T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.
T1 0-103 Sentence denotes A new glycoengineered insect cell line with an inducibly mammalianized protein N-glycosylation pathway.
T2 104-244 Sentence denotes The inability to produce recombinant glycoproteins with authentic N-glycans is a limitation of many heterologous protein expression systems.
T3 245-496 Sentence denotes In the baculovirus-insect cell system, this limitation has been addressed by glycoengineering insect cell lines with mammalian genes encoding protein N-glycosylation functions ("glycogenes") under the transcriptional control of constitutive promoters.
T4 497-709 Sentence denotes However, a potential problem with this approach is that the metabolic load imposed by the expression of multiple transgenes could adversely impact the growth and/or stability of glycoengineered insect cell lines.
T5 710-835 Sentence denotes Thus, we created a new transgenic insect cell line (SfSWT-5) with an inducibly mammalianized protein N-glycosylation pathway.
T6 836-965 Sentence denotes Expression of all six glycogenes was induced when uninfected SfSWT-5 cells were cultured in growth medium containing doxycycline.
T7 966-1104 Sentence denotes Higher levels of expression and induction were observed when SfSWT-5 cells were cultured with doxycycline and infected with a baculovirus.
T8 1105-1242 Sentence denotes Interestingly, there were no major differences in the short-term growth properties of SfSWT-5 cells cultured with or without doxycycline.
T9 1243-1406 Sentence denotes Furthermore, there were no major differences in the phenotypic stability of these cells after continuous culture for over 300 passages with or without doxycycline.
T10 1407-1644 Sentence denotes Baculovirus-infected Sf9 and SfSWT-5 cells produced about the same amounts of a model recombinant glycoprotein, but only the latter sialylated this product and sialylation was more pronounced when the cells were treated with doxycycline.
T11 1645-1914 Sentence denotes In summary, this is the first report of a lower eukaryotic system with an inducibly mammalianized protein N-glycosylation pathway and the first to examine how the presumed metabolic load imposed by multiple transgene expression impacts insect cell growth and stability.

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 71-78 FMAID:165447 denotes protein
_T2 71-78 FMAID:67257 denotes protein
_T3 141-154 FMAID:62925 denotes glycoproteins
_T4 141-154 FMAID:167256 denotes glycoproteins
_T5 217-224 FMAID:67257 denotes protein
_T6 217-224 FMAID:165447 denotes protein
_T7 372-377 FMAID:198663 denotes genes
_T8 387-394 FMAID:165447 denotes protein
_T9 387-394 FMAID:67257 denotes protein
_T10 423-433 FMAID:82747 denotes glycogenes
_T11 423-433 FMAID:196736 denotes glycogenes
_T12 803-810 FMAID:165447 denotes protein
_T13 803-810 FMAID:67257 denotes protein
_T14 858-868 FMAID:82747 denotes glycogenes
_T15 858-868 FMAID:196736 denotes glycogenes
_T16 905-910 FMAID:68646 denotes cells
_T17 905-910 FMAID:169002 denotes cells
_T18 1035-1040 FMAID:169002 denotes cells
_T19 1035-1040 FMAID:68646 denotes cells
_T20 1199-1204 FMAID:169002 denotes cells
_T21 1199-1204 FMAID:68646 denotes cells
_T22 1325-1330 FMAID:169002 denotes cells
_T23 1325-1330 FMAID:68646 denotes cells
_T24 1444-1449 FMAID:68646 denotes cells
_T25 1444-1449 FMAID:169002 denotes cells
_T26 1505-1517 FMAID:167256 denotes glycoprotein
_T27 1505-1517 FMAID:62925 denotes glycoprotein
_T28 1532-1538 FMAID:30332 denotes latter
_T29 1532-1538 FMAID:171168 denotes latter
_T30 1608-1613 FMAID:68646 denotes cells
_T31 1608-1613 FMAID:169002 denotes cells
_T32 1743-1750 FMAID:165447 denotes protein
_T33 1743-1750 FMAID:67257 denotes protein

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 199-203 http://purl.bioontology.org/ontology/NCBITAXON/9973 denotes many
T2 252-263 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes baculovirus
T3 905-910 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T4 1035-1040 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T5 1092-1103 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes baculovirus
T6 1165-1169 http://purl.bioontology.org/ontology/NCBITAXON/62951 denotes term
T7 1199-1204 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T8 1325-1330 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T9 1407-1418 http://purl.bioontology.org/ontology/NCBITAXON/558017 denotes Baculovirus
T10 1444-1449 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T11 1608-1613 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T12 1693-1703 http://purl.bioontology.org/ontology/NCBITAXON/2759 denotes eukaryotic

GO-BP

Id Subject Object Predicate Lexical cue
T1 81-94 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T2 397-410 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T3 813-826 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T4 1753-1766 http://purl.obolibrary.org/obo/GO_0070085 denotes glycosylation
T5 446-461 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T6 446-469 http://purl.obolibrary.org/obo/GO_0006355 denotes transcriptional control
T7 557-566 http://purl.obolibrary.org/obo/GO_0008152 denotes metabolic
T8 1817-1826 http://purl.obolibrary.org/obo/GO_0008152 denotes metabolic
T9 648-654 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T10 928-934 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T11 1170-1176 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T12 1893-1899 http://purl.obolibrary.org/obo/GO_0040007 denotes growth
T13 1539-1549 http://purl.obolibrary.org/obo/GO_0097503 denotes sialylated
T14 1567-1578 http://purl.obolibrary.org/obo/GO_0097503 denotes sialylation
T15 1888-1899 http://purl.obolibrary.org/obo/GO_0016049 denotes cell growth

GO-CC

Id Subject Object Predicate Lexical cue
T1 29-33 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2 271-275 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3 346-350 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4 698-702 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5 751-755 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T6 905-910 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7 1035-1040 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8 1199-1204 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9 1325-1330 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10 1444-1449 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11 1608-1613 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GlycoBiology-MAT

Id Subject Object Predicate Lexical cue
T1 1532-1538 http://purl.obolibrary.org/obo/MAT_0000488 denotes latter

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 850-853 "http://rdf.glycoinfo.org/glycan/G41652MJ" denotes all
GlycanIUPAC_T2 850-853 "http://rdf.glycoinfo.org/glycan/G20761YC" denotes all
GlycanIUPAC_T3 850-853 "http://rdf.glycoinfo.org/glycan/G19807HM" denotes all
GlycanIUPAC_T4 850-853 "http://rdf.glycoinfo.org/glycan/G20351TE" denotes all
GlycanIUPAC_T5 850-853 "http://rdf.glycoinfo.org/glycan/G71957MR" denotes all
GlycanIUPAC_T6 850-853 "http://rdf.glycoinfo.org/glycan/G59040AE" denotes all
GlycanIUPAC_T7 850-853 "http://rdf.glycoinfo.org/glycan/G14987PW" denotes all
GlycanIUPAC_T8 850-853 "http://rdf.glycoinfo.org/glycan/G95064PC" denotes all
GlycanIUPAC_T9 850-853 "http://rdf.glycoinfo.org/glycan/G39143AQ" denotes all
GlycanIUPAC_T10 850-853 "http://rdf.glycoinfo.org/glycan/G65149OO" denotes all
GlycanIUPAC_T11 850-853 "http://rdf.glycoinfo.org/glycan/G02766SY" denotes all
GlycanIUPAC_T12 850-853 "http://rdf.glycoinfo.org/glycan/G26019KJ" denotes all
GlycanIUPAC_T13 850-853 "http://rdf.glycoinfo.org/glycan/G36429CZ" denotes all
GlycanIUPAC_T14 850-853 "http://rdf.glycoinfo.org/glycan/G89633TP" denotes all
GlycanIUPAC_T15 850-853 "http://rdf.glycoinfo.org/glycan/G28494FO" denotes all
GlycanIUPAC_T16 850-853 "http://rdf.glycoinfo.org/glycan/G06219CP" denotes all
GlycanIUPAC_T17 850-853 "http://rdf.glycoinfo.org/glycan/G44237SM" denotes all
GlycanIUPAC_T18 850-853 "http://rdf.glycoinfo.org/glycan/G57948RL" denotes all
GlycanIUPAC_T19 850-853 "http://rdf.glycoinfo.org/glycan/G64016DN" denotes all
GlycanIUPAC_T20 850-853 "http://rdf.glycoinfo.org/glycan/G14536PC" denotes all
GlycanIUPAC_T21 850-853 "http://rdf.glycoinfo.org/glycan/G14356FW" denotes all
GlycanIUPAC_T22 850-853 "http://rdf.glycoinfo.org/glycan/G34565UO" denotes all
GlycanIUPAC_T23 850-853 "http://rdf.glycoinfo.org/glycan/G67124MW" denotes all
GlycanIUPAC_T24 850-853 "http://rdf.glycoinfo.org/glycan/G71457ZU" denotes all
GlycanIUPAC_T25 850-853 "http://rdf.glycoinfo.org/glycan/G55228VZ" denotes all
GlycanIUPAC_T26 850-853 "http://rdf.glycoinfo.org/glycan/G31034MJ" denotes all
GlycanIUPAC_T27 850-853 "http://rdf.glycoinfo.org/glycan/G25776IP" denotes all
GlycanIUPAC_T28 850-853 "http://rdf.glycoinfo.org/glycan/G64442BV" denotes all
GlycanIUPAC_T29 850-853 "http://rdf.glycoinfo.org/glycan/G57018LE" denotes all
GlycanIUPAC_T30 850-853 "http://rdf.glycoinfo.org/glycan/G61761GX" denotes all
GlycanIUPAC_T31 850-853 "http://rdf.glycoinfo.org/glycan/G76318UX" denotes all
GlycanIUPAC_T32 850-853 "http://rdf.glycoinfo.org/glycan/G61906ER" denotes all
GlycanIUPAC_T33 850-853 "http://rdf.glycoinfo.org/glycan/G68723GR" denotes all
GlycanIUPAC_T34 850-853 "http://rdf.glycoinfo.org/glycan/G19540LE" denotes all
GlycanIUPAC_T35 850-853 "http://rdf.glycoinfo.org/glycan/G74944PO" denotes all
GlycanIUPAC_T36 850-853 "http://rdf.glycoinfo.org/glycan/G89489ZJ" denotes all
GlycanIUPAC_T37 850-853 "http://rdf.glycoinfo.org/glycan/G04434YU" denotes all
GlycanIUPAC_T38 850-853 "http://rdf.glycoinfo.org/glycan/G21450PB" denotes all
GlycanIUPAC_T39 850-853 "http://rdf.glycoinfo.org/glycan/G93629QY" denotes all
GlycanIUPAC_T40 850-853 "http://rdf.glycoinfo.org/glycan/G02603TR" denotes all
GlycanIUPAC_T41 850-853 "http://rdf.glycoinfo.org/glycan/G40280JP" denotes all
GlycanIUPAC_T42 850-853 "http://rdf.glycoinfo.org/glycan/G95259IC" denotes all
GlycanIUPAC_T43 850-853 "http://rdf.glycoinfo.org/glycan/G26900FE" denotes all
GlycanIUPAC_T44 850-853 "http://rdf.glycoinfo.org/glycan/G21346KK" denotes all
GlycanIUPAC_T45 850-853 "http://rdf.glycoinfo.org/glycan/G62509FF" denotes all
GlycanIUPAC_T46 850-853 "http://rdf.glycoinfo.org/glycan/G83932AK" denotes all
GlycanIUPAC_T47 850-853 "http://rdf.glycoinfo.org/glycan/G96978IB" denotes all
GlycanIUPAC_T48 850-853 "http://rdf.glycoinfo.org/glycan/G34275DN" denotes all
GlycanIUPAC_T49 850-853 "http://rdf.glycoinfo.org/glycan/G07071JF" denotes all
GlycanIUPAC_T50 850-853 "http://rdf.glycoinfo.org/glycan/G80639QD" denotes all
GlycanIUPAC_T51 850-853 "http://rdf.glycoinfo.org/glycan/G99460PJ" denotes all
GlycanIUPAC_T52 850-853 "http://rdf.glycoinfo.org/glycan/G22024BZ" denotes all
GlycanIUPAC_T53 850-853 "http://rdf.glycoinfo.org/glycan/G74097ZY" denotes all
GlycanIUPAC_T54 850-853 "http://rdf.glycoinfo.org/glycan/G84439YP" denotes all
GlycanIUPAC_T55 850-853 "http://rdf.glycoinfo.org/glycan/G52207WQ" denotes all
GlycanIUPAC_T56 850-853 "http://rdf.glycoinfo.org/glycan/G90695MS" denotes all
GlycanIUPAC_T57 850-853 "http://rdf.glycoinfo.org/glycan/G50398QX" denotes all
GlycanIUPAC_T58 850-853 "http://rdf.glycoinfo.org/glycan/G12166ZT" denotes all
GlycanIUPAC_T59 850-853 "http://rdf.glycoinfo.org/glycan/G48368BR" denotes all
GlycanIUPAC_T60 850-853 "http://rdf.glycoinfo.org/glycan/G57407RW" denotes all
GlycanIUPAC_T61 850-853 "http://rdf.glycoinfo.org/glycan/G00386TY" denotes all
GlycanIUPAC_T62 850-853 "http://rdf.glycoinfo.org/glycan/G18723JK" denotes all
GlycanIUPAC_T63 850-853 "http://rdf.glycoinfo.org/glycan/G93757OR" denotes all
GlycanIUPAC_T64 850-853 "http://rdf.glycoinfo.org/glycan/G29006SI" denotes all
GlycanIUPAC_T65 850-853 "http://rdf.glycoinfo.org/glycan/G03099OQ" denotes all
GlycanIUPAC_T66 850-853 "http://rdf.glycoinfo.org/glycan/G53739OW" denotes all
GlycanIUPAC_T67 850-853 "http://rdf.glycoinfo.org/glycan/G70440ZO" denotes all
GlycanIUPAC_T68 850-853 "http://rdf.glycoinfo.org/glycan/G29951RR" denotes all
GlycanIUPAC_T69 850-853 "http://rdf.glycoinfo.org/glycan/G58402TI" denotes all
GlycanIUPAC_T70 850-853 "http://rdf.glycoinfo.org/glycan/G39875TP" denotes all
GlycanIUPAC_T71 850-853 "http://rdf.glycoinfo.org/glycan/G83439QV" denotes all
GlycanIUPAC_T72 850-853 "http://rdf.glycoinfo.org/glycan/G41762RC" denotes all
GlycanIUPAC_T73 850-853 "http://rdf.glycoinfo.org/glycan/G91604UI" denotes all
GlycanIUPAC_T74 850-853 "http://rdf.glycoinfo.org/glycan/G88447WE" denotes all
GlycanIUPAC_T75 850-853 "http://rdf.glycoinfo.org/glycan/G93634BS" denotes all
GlycanIUPAC_T76 850-853 "http://rdf.glycoinfo.org/glycan/G02587BH" denotes all
GlycanIUPAC_T77 850-853 "http://rdf.glycoinfo.org/glycan/G43511MX" denotes all
GlycanIUPAC_T78 850-853 "http://rdf.glycoinfo.org/glycan/G64958DH" denotes all
GlycanIUPAC_T79 850-853 "http://rdf.glycoinfo.org/glycan/G30384TR" denotes all
GlycanIUPAC_T80 850-853 "http://rdf.glycoinfo.org/glycan/G15624EX" denotes all
GlycanIUPAC_T81 850-853 "http://rdf.glycoinfo.org/glycan/G22706ST" denotes all
GlycanIUPAC_T82 850-853 "http://rdf.glycoinfo.org/glycan/G57408PI" denotes all
GlycanIUPAC_T83 850-853 "http://rdf.glycoinfo.org/glycan/G86403XX" denotes all
GlycanIUPAC_T84 850-853 "http://rdf.glycoinfo.org/glycan/G78043YB" denotes all
GlycanIUPAC_T85 850-853 "http://rdf.glycoinfo.org/glycan/G18952JK" denotes all
GlycanIUPAC_T86 850-853 "http://rdf.glycoinfo.org/glycan/G49020ND" denotes all
GlycanIUPAC_T87 850-853 "http://rdf.glycoinfo.org/glycan/G63590YW" denotes all
GlycanIUPAC_T88 850-853 "http://rdf.glycoinfo.org/glycan/G22793KS" denotes all
GlycanIUPAC_T89 850-853 "http://rdf.glycoinfo.org/glycan/G64134SS" denotes all
GlycanIUPAC_T90 850-853 "http://rdf.glycoinfo.org/glycan/G17338HY" denotes all
GlycanIUPAC_T91 850-853 "http://rdf.glycoinfo.org/glycan/G99745XF" denotes all
GlycanIUPAC_T92 850-853 "http://rdf.glycoinfo.org/glycan/G27782HN" denotes all
GlycanIUPAC_T93 850-853 "http://rdf.glycoinfo.org/glycan/G57496DC" denotes all
GlycanIUPAC_T94 850-853 "http://rdf.glycoinfo.org/glycan/G93169WB" denotes all
GlycanIUPAC_T95 850-853 "http://rdf.glycoinfo.org/glycan/G05518TD" denotes all
GlycanIUPAC_T96 850-853 "http://rdf.glycoinfo.org/glycan/G62603DN" denotes all
GlycanIUPAC_T97 850-853 "http://rdf.glycoinfo.org/glycan/G59574FS" denotes all
GlycanIUPAC_T98 850-853 "http://rdf.glycoinfo.org/glycan/G47567WC" denotes all

GlycoBiology-Motifs

Id Subject Object Predicate Lexical cue
T1 170-179 http://rdf.glycoinfo.org/glycan/G00027MO denotes N-glycans