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PubMed:22033932 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-103 Sentence denotes Probing binding pocket of serotonin transporter by single molecular force spectroscopy on living cells.
T2 104-212 Sentence denotes The serotonin transporter (SERT) terminates neurotransmission by removing serotonin from the synaptic cleft.
T3 213-352 Sentence denotes In addition, it is the site of action of antidepressants (which block the transporter) and of amphetamines (which induce substrate efflux).
T4 353-447 Sentence denotes The interaction energies involved in binding of such compounds to the transporter are unknown.
T5 448-625 Sentence denotes Here, we used atomic force microscopy (AFM) to probe single molecular interactions between the serotonin transporter and MFZ2-12 (a potent cocaine analog) in living CHOK1 cells.
T6 626-731 Sentence denotes For the AFM measurements, MFZ2-12 was immobilized on AFM tips by using a heterobifunctional cross-linker.
T7 732-915 Sentence denotes By varying the pulling velocity in force distance cycles drug-transporter complexes were ruptured at different force loadings allowing for mapping of the interaction energy landscape.
T8 916-1207 Sentence denotes We derived chemical rate constants from these recordings and compared them with those inferred from inhibition of transport and ligand binding: koff values were in good agreement with those derived from uptake experiments; in contrast, the kon values were scaled down when determined by AFM.
T9 1208-1321 Sentence denotes Our observations generated new insights into the energy landscape of the interaction between SERT and inhibitors.
T10 1322-1481 Sentence denotes They thus provide a useful framework for molecular dynamics simulations by exploring the range of forces and energies that operate during the binding reaction.

NEUROSES

Id Subject Object Predicate Lexical cue
T1 26-35 CHEBI_350546 denotes serotonin
T2 108-117 CHEBI_350546 denotes serotonin
T3 178-187 CHEBI_350546 denotes serotonin
T4 543-552 CHEBI_350546 denotes serotonin
T5 26-35 CHEBI_28790 denotes serotonin
T6 108-117 CHEBI_28790 denotes serotonin
T7 178-187 CHEBI_28790 denotes serotonin
T8 543-552 CHEBI_28790 denotes serotonin
T9 68-73 PATO_0001035 denotes force
T10 469-474 PATO_0001035 denotes force
T11 767-772 PATO_0001035 denotes force
T12 843-848 PATO_0001035 denotes force
T13 206-211 PATO_0000403 denotes cleft
T15 307-319 CHEBI_35338 denotes amphetamines
T16 495-500 CHEBI_50406 denotes probe
T17 587-594 CHEBI_27958 denotes cocaine
T18 587-594 CHEBI_60056 denotes cocaine
T19 755-763 PATO_0000008 denotes velocity
T20 755-763 PATO_0002242 denotes velocity
T21 773-781 PATO_0000040 denotes distance
T22 789-793 CHEBI_23888 denotes drug
T23 821-829 PATO_0001444 denotes ruptured
T24 898-904 PATO_0001021 denotes energy
T25 1257-1263 PATO_0001021 denotes energy
T26 936-940 PATO_0001470 denotes rate
T27 936-940 PATO_0000161 denotes rate
T28 1044-1050 CHEBI_52214 denotes ligand
T29 1310-1320 CHEBI_35222 denotes inhibitors

Allie

Id Subject Object Predicate Lexical cue
SS1_22033932_1_0 108-129 expanded denotes serotonin transporter
SS2_22033932_1_0 131-135 abbr denotes SERT
SS1_22033932_4_0 462-485 expanded denotes atomic force microscopy
SS2_22033932_4_0 487-490 abbr denotes AFM
AE1_22033932_1_0 SS1_22033932_1_0 SS2_22033932_1_0 abbreviatedTo serotonin transporter,SERT
AE1_22033932_4_0 SS1_22033932_4_0 SS2_22033932_4_0 abbreviatedTo atomic force microscopy,AFM