| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-136 |
Sentence |
denotes |
Serine protease autotransporters from Shigella flexneri and pathogenic Escherichia coli target a broad range of leukocyte glycoproteins. |
| T2 |
137-291 |
Sentence |
denotes |
The serine protease autotransporters of Enterobacteriaceae (SPATEs) are secreted by pathogenic Gram-negative bacteria through the autotransporter pathway. |
| T3 |
292-397 |
Sentence |
denotes |
We previously classified SPATE proteins into two classes: cytotoxic (class 1) and noncytotoxic (class 2). |
| T4 |
398-601 |
Sentence |
denotes |
Here, we show that Pic, a class 2 SPATE protein produced by Shigella flexneri 2a, uropathogenic and enteroaggregative Escherichia coli strains, targets a broad range of human leukocyte adhesion proteins. |
| T5 |
602-883 |
Sentence |
denotes |
Substrate specificity was restricted to glycoproteins rich in O-linked glycans, including CD43, CD44, CD45, CD93, CD162 (PSGL-1; P-selectin glycoprotein ligand 1), and the surface-attached chemokine fractalkine, all implicated in leukocyte trafficking, migration, and inflammation. |
| T6 |
884-1030 |
Sentence |
denotes |
N-terminal sequencing of proteolytic products revealed Pic (protease involved in colonization) cleavage sites to occur before Thr or Ser residues. |
| T7 |
1031-1141 |
Sentence |
denotes |
The purified carbohydrate sLewis-X implied in inflammation and malignancy inhibited cleavage of PSGL-1 by Pic. |
| T8 |
1142-1330 |
Sentence |
denotes |
Exposure of human leukocytes to purified Pic resulted in polymorphonuclear cell activation, but impaired chemotaxis and transmigration; Pic-treated T cells underwent programmed cell death. |
| T9 |
1331-1492 |
Sentence |
denotes |
We also show that the Pic-related protease Tsh/Hbp, implicated in extraintestinal infections, exhibited a spectrum of substrates similar to those cleaved by Pic. |
| T10 |
1493-1613 |
Sentence |
denotes |
In the guinea pig keratoconjunctivitis model, a Shigella pic mutant induced greater inflammation than its parent strain. |
| T11 |
1614-1712 |
Sentence |
denotes |
We suggest that the class-2 SPATEs represent unique immune-modulating bacterial virulence factors. |
| T1 |
0-136 |
Sentence |
denotes |
Serine protease autotransporters from Shigella flexneri and pathogenic Escherichia coli target a broad range of leukocyte glycoproteins. |
| T2 |
137-291 |
Sentence |
denotes |
The serine protease autotransporters of Enterobacteriaceae (SPATEs) are secreted by pathogenic Gram-negative bacteria through the autotransporter pathway. |
| T3 |
292-397 |
Sentence |
denotes |
We previously classified SPATE proteins into two classes: cytotoxic (class 1) and noncytotoxic (class 2). |
| T4 |
398-601 |
Sentence |
denotes |
Here, we show that Pic, a class 2 SPATE protein produced by Shigella flexneri 2a, uropathogenic and enteroaggregative Escherichia coli strains, targets a broad range of human leukocyte adhesion proteins. |
| T5 |
602-883 |
Sentence |
denotes |
Substrate specificity was restricted to glycoproteins rich in O-linked glycans, including CD43, CD44, CD45, CD93, CD162 (PSGL-1; P-selectin glycoprotein ligand 1), and the surface-attached chemokine fractalkine, all implicated in leukocyte trafficking, migration, and inflammation. |
| T6 |
884-1030 |
Sentence |
denotes |
N-terminal sequencing of proteolytic products revealed Pic (protease involved in colonization) cleavage sites to occur before Thr or Ser residues. |
| T7 |
1031-1141 |
Sentence |
denotes |
The purified carbohydrate sLewis-X implied in inflammation and malignancy inhibited cleavage of PSGL-1 by Pic. |
| T8 |
1142-1330 |
Sentence |
denotes |
Exposure of human leukocytes to purified Pic resulted in polymorphonuclear cell activation, but impaired chemotaxis and transmigration; Pic-treated T cells underwent programmed cell death. |
| T9 |
1331-1492 |
Sentence |
denotes |
We also show that the Pic-related protease Tsh/Hbp, implicated in extraintestinal infections, exhibited a spectrum of substrates similar to those cleaved by Pic. |
| T10 |
1493-1613 |
Sentence |
denotes |
In the guinea pig keratoconjunctivitis model, a Shigella pic mutant induced greater inflammation than its parent strain. |
| T11 |
1614-1712 |
Sentence |
denotes |
We suggest that the class-2 SPATEs represent unique immune-modulating bacterial virulence factors. |