PubMed:21762900 JSONTXT

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":0,"end":14},"obj":"gene:3553"},{"id":"T1","span":{"begin":130,"end":150},"obj":"disease:C0269102"},{"id":"T2","span":{"begin":23,"end":39},"obj":"gene:5743"},{"id":"T3","span":{"begin":130,"end":150},"obj":"disease:C0269102"},{"id":"T4","span":{"begin":188,"end":202},"obj":"gene:3553"},{"id":"T5","span":{"begin":266,"end":278},"obj":"disease:C0269102"},{"id":"T6","span":{"begin":214,"end":230},"obj":"gene:5743"},{"id":"T7","span":{"begin":266,"end":278},"obj":"disease:C0269102"},{"id":"T8","span":{"begin":232,"end":237},"obj":"gene:5743"},{"id":"T9","span":{"begin":266,"end":278},"obj":"disease:C0269102"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"},{"id":"R5","pred":"associated_with","subj":"T8","obj":"T9"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Interleukin-1β induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma.\nOBJECTIVE: To elucidate the role of interleukin-1β (IL-1β) on cyclooxygenase-2 (COX-2) expression and invasion of endometrioma-derived ectopic endometrial mesenchymal stem cells (EN-MSCs) and to develop an organoid method to study the invasive ability of endometrial cells.\nDESIGN: Gene expression and cell functions.\nSETTING: Kaohsiung Medical University, Kaohsiung, Taiwan.\nPATIENT(S): Human eutopic and endometrioma-derived ectopic EN-MSCs were isolated from different endometrium biopsy samples after surgery for treatment of endometriosis.\nINTERVENTION(S): Chemical treatment of cell culture.\nMAIN OUTCOME MEASURE(S): Comparative analysis of genomewide messenger RNA (mRNA) expression, cell migration, and invasion abilities in cell culture and organoid culture.\nRESULT(S): Gene expression profiles revealed that the expression of IL-1β and COX-2 were statistically significantly higher in ectopic EN-MSCs compared with eutopic EN-MSCs. These enhanced expressions coincided with a greater ability for cell migration and invasion in ectopic EN-MSCs and were found to be distinctly regulated by IL-1β which up-regulates COX-2 expression. Furthermore, IL-1β treatment of ectopic EN-MSCs in organoids was found to induce tentacle-like structures that mimicked cell invasion.\nCONCLUSION(S): These results indicate that COX-2 and IL-1β regulate the invasion ability of ectopic EN-MSCs. The information may be useful for developing a new therapeutic strategy for endometriosis. The ex vivo invasion model will be useful for characterization of EN-MSCs."}

{"project":"Allie","denotations":[{"id":"SS1_21762900_2_0","span":{"begin":188,"end":202},"obj":"expanded"},{"id":"SS2_21762900_2_0","span":{"begin":204,"end":209},"obj":"abbr"},{"id":"SS1_21762900_2_1","span":{"begin":214,"end":230},"obj":"expanded"},{"id":"SS2_21762900_2_1","span":{"begin":232,"end":237},"obj":"abbr"},{"id":"SS1_21762900_2_2","span":{"begin":295,"end":329},"obj":"expanded"},{"id":"SS2_21762900_2_2","span":{"begin":331,"end":338},"obj":"abbr"},{"id":"SS1_21762900_12_0","span":{"begin":810,"end":823},"obj":"expanded"},{"id":"SS2_21762900_12_0","span":{"begin":825,"end":829},"obj":"abbr"}],"relations":[{"id":"AE1_21762900_2_0","pred":"abbreviatedTo","subj":"SS1_21762900_2_0","obj":"SS2_21762900_2_0"},{"id":"AE1_21762900_2_1","pred":"abbreviatedTo","subj":"SS1_21762900_2_1","obj":"SS2_21762900_2_1"},{"id":"AE1_21762900_2_2","pred":"abbreviatedTo","subj":"SS1_21762900_2_2","obj":"SS2_21762900_2_2"},{"id":"AE1_21762900_12_0","pred":"abbreviatedTo","subj":"SS1_21762900_12_0","obj":"SS2_21762900_12_0"}],"text":"Interleukin-1β induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma.\nOBJECTIVE: To elucidate the role of interleukin-1β (IL-1β) on cyclooxygenase-2 (COX-2) expression and invasion of endometrioma-derived ectopic endometrial mesenchymal stem cells (EN-MSCs) and to develop an organoid method to study the invasive ability of endometrial cells.\nDESIGN: Gene expression and cell functions.\nSETTING: Kaohsiung Medical University, Kaohsiung, Taiwan.\nPATIENT(S): Human eutopic and endometrioma-derived ectopic EN-MSCs were isolated from different endometrium biopsy samples after surgery for treatment of endometriosis.\nINTERVENTION(S): Chemical treatment of cell culture.\nMAIN OUTCOME MEASURE(S): Comparative analysis of genomewide messenger RNA (mRNA) expression, cell migration, and invasion abilities in cell culture and organoid culture.\nRESULT(S): Gene expression profiles revealed that the expression of IL-1β and COX-2 were statistically significantly higher in ectopic EN-MSCs compared with eutopic EN-MSCs. These enhanced expressions coincided with a greater ability for cell migration and invasion in ectopic EN-MSCs and were found to be distinctly regulated by IL-1β which up-regulates COX-2 expression. Furthermore, IL-1β treatment of ectopic EN-MSCs in organoids was found to induce tentacle-like structures that mimicked cell invasion.\nCONCLUSION(S): These results indicate that COX-2 and IL-1β regulate the invasion ability of ectopic EN-MSCs. The information may be useful for developing a new therapeutic strategy for endometriosis. The ex vivo invasion model will be useful for characterization of EN-MSCs."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"21762900-0#0#14#gene3553","span":{"begin":0,"end":14},"obj":"gene3553"},{"id":"21762900-0#23#39#gene5743","span":{"begin":23,"end":39},"obj":"gene5743"},{"id":"21762900-0#130#150#diseaseC0269102","span":{"begin":130,"end":150},"obj":"diseaseC0269102"},{"id":"21762900-4#50#53#gene9242","span":{"begin":590,"end":593},"obj":"gene9242"},{"id":"21762900-4#18#30#diseaseC0269102","span":{"begin":558,"end":570},"obj":"diseaseC0269102"},{"id":"21762900-4#142#155#diseaseC0014175","span":{"begin":682,"end":695},"obj":"diseaseC0014175"}],"relations":[{"id":"0#14#gene3553130#150#diseaseC0269102","pred":"associated_with","subj":"21762900-0#0#14#gene3553","obj":"21762900-0#130#150#diseaseC0269102"},{"id":"23#39#gene5743130#150#diseaseC0269102","pred":"associated_with","subj":"21762900-0#23#39#gene5743","obj":"21762900-0#130#150#diseaseC0269102"},{"id":"50#53#gene924218#30#diseaseC0269102","pred":"associated_with","subj":"21762900-4#50#53#gene9242","obj":"21762900-4#18#30#diseaseC0269102"},{"id":"50#53#gene9242142#155#diseaseC0014175","pred":"associated_with","subj":"21762900-4#50#53#gene9242","obj":"21762900-4#142#155#diseaseC0014175"}],"text":"Interleukin-1β induces cyclooxygenase-2 expression and promotes the invasive ability of human mesenchymal stem cells derived from ovarian endometrioma.\nOBJECTIVE: To elucidate the role of interleukin-1β (IL-1β) on cyclooxygenase-2 (COX-2) expression and invasion of endometrioma-derived ectopic endometrial mesenchymal stem cells (EN-MSCs) and to develop an organoid method to study the invasive ability of endometrial cells.\nDESIGN: Gene expression and cell functions.\nSETTING: Kaohsiung Medical University, Kaohsiung, Taiwan.\nPATIENT(S): Human eutopic and endometrioma-derived ectopic EN-MSCs were isolated from different endometrium biopsy samples after surgery for treatment of endometriosis.\nINTERVENTION(S): Chemical treatment of cell culture.\nMAIN OUTCOME MEASURE(S): Comparative analysis of genomewide messenger RNA (mRNA) expression, cell migration, and invasion abilities in cell culture and organoid culture.\nRESULT(S): Gene expression profiles revealed that the expression of IL-1β and COX-2 were statistically significantly higher in ectopic EN-MSCs compared with eutopic EN-MSCs. These enhanced expressions coincided with a greater ability for cell migration and invasion in ectopic EN-MSCs and were found to be distinctly regulated by IL-1β which up-regulates COX-2 expression. Furthermore, IL-1β treatment of ectopic EN-MSCs in organoids was found to induce tentacle-like structures that mimicked cell invasion.\nCONCLUSION(S): These results indicate that COX-2 and IL-1β regulate the invasion ability of ectopic EN-MSCs. The information may be useful for developing a new therapeutic strategy for endometriosis. The ex vivo invasion model will be useful for characterization of EN-MSCs."}