PubMed:21737620 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/21737620","sourcedb":"PubMed","sourceid":"21737620","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/21737620","text":"Overexpression of kappa-actin alters growth properties of hepatoma cells and predicts poor postoperative prognosis.\nUNLABELLED: Since Rho-mediated signaling can regulate liver cancer cell proliferation, amino acid sequence changes of its downstream targets, actins, might alter the properties of cell growth. Here, we investigated the function of a novel class of actins, named κ-actins, in hepatocellular carcinoma (HCC).\nMATERIALS AND METHODS: Alexander cells overexpressing an HCC-derived κ-actin (Alex-κ cells) were established to study growth property changes. κ-actin expression was also determined in tumor and noncancerous tissues from 72 HCC patients. Survival analysis was conducted to evaluate the prognostic predictive value of κ-actin expression.\nRESULTS: Phylogenetic analysis showed that κ-actin sequences constituted 94.7% and 17.6% of actin transcripts in Alex-κ and naive Alexander cells, respectively. Alex-κ cells exhibited serum-independent cell growth with increased anchorage-independent colony formation and BrdU incorporation upon serum deprivation. Cox proportional hazard analysis showed that κ-actin expression in both cancerous and noncancerous tissues predicted poorer postoperative disease-free survival (p=0.004).\nCONCLUSION: Overexpression of κ-actin altered growth properties of hepatoma cells, contributing to poor postoperative prognosis.","tracks":[{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":18,"end":29},"obj":"gene:345651"},{"id":"T1","span":{"begin":58,"end":66},"obj":"disease:C0023903"},{"id":"T2","span":{"begin":18,"end":29},"obj":"gene:345651"},{"id":"T3","span":{"begin":58,"end":66},"obj":"disease:C2239176"},{"id":"T4","span":{"begin":18,"end":29},"obj":"gene:440915"},{"id":"T5","span":{"begin":58,"end":66},"obj":"disease:C0023903"},{"id":"T6","span":{"begin":18,"end":29},"obj":"gene:440915"},{"id":"T7","span":{"begin":58,"end":66},"obj":"disease:C2239176"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"},{"id":"R4","pred":"associated_with","subj":"T6","obj":"T7"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"},{"subj":"T2","pred":"source","obj":"DisGeNET"},{"subj":"T3","pred":"source","obj":"DisGeNET"},{"subj":"T4","pred":"source","obj":"DisGeNET"},{"subj":"T5","pred":"source","obj":"DisGeNET"},{"subj":"T6","pred":"source","obj":"DisGeNET"},{"subj":"T7","pred":"source","obj":"DisGeNET"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":170,"end":182},"obj":"HP_0002896"},{"id":"T2","span":{"begin":176,"end":182},"obj":"HP_0002664"},{"id":"T3","span":{"begin":391,"end":415},"obj":"HP_0001402"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"}]},{"project":"Allie","denotations":[{"id":"SS1_21737620_3_0","span":{"begin":391,"end":415},"obj":"expanded"},{"id":"SS2_21737620_3_0","span":{"begin":417,"end":420},"obj":"abbr"}],"relations":[{"id":"AE1_21737620_3_0","pred":"abbreviatedTo","subj":"SS1_21737620_3_0","obj":"SS2_21737620_3_0"}],"attributes":[{"subj":"SS1_21737620_3_0","pred":"source","obj":"Allie"},{"subj":"SS2_21737620_3_0","pred":"source","obj":"Allie"}]},{"project":"tutorial1","denotations":[{"id":"T1","span":{"begin":170,"end":175},"obj":"http://purl.obolibrary.org/obo/UBERON_0002107"},{"id":"T2","span":{"begin":631,"end":638},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T3","span":{"begin":1174,"end":1181},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T4","span":{"begin":944,"end":949},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T5","span":{"begin":1056,"end":1061},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"attributes":[{"subj":"T1","pred":"source","obj":"tutorial1"},{"subj":"T2","pred":"source","obj":"tutorial1"},{"subj":"T3","pred":"source","obj":"tutorial1"},{"subj":"T4","pred":"source","obj":"tutorial1"},{"subj":"T5","pred":"source","obj":"tutorial1"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"DisGeNET","color":"#ec93e4","default":true},{"id":"PubmedHPO","color":"#93ecda"},{"id":"Allie","color":"#ecc093"},{"id":"tutorial1","color":"#a693ec"}]}]}}