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Allie

Id Subject Object Predicate Lexical cue
SS1_21697323_1_0 354-380 expanded denotes quantitative real-time PCR
SS2_21697323_1_0 382-389 abbr denotes QRT-PCR
SS1_21697323_1_1 423-438 expanded denotes cytomegalovirus
SS2_21697323_1_1 440-443 abbr denotes CMV
SS1_21697323_2_0 884-915 expanded denotes allogeneic stem cell transplant
SS2_21697323_2_0 917-925 abbr denotes Allo-SCT
AE1_21697323_1_0 SS1_21697323_1_0 SS2_21697323_1_0 abbreviatedTo quantitative real-time PCR,QRT-PCR
AE1_21697323_1_1 SS1_21697323_1_1 SS2_21697323_1_1 abbreviatedTo cytomegalovirus,CMV
AE1_21697323_2_0 SS1_21697323_2_0 SS2_21697323_2_0 abbreviatedTo allogeneic stem cell transplant,Allo-SCT

Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-236 Sentence denotes Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma Cytomegalovirus DNAemia in allogeneic stem cell transplant recipients.
T2 237-459 Sentence denotes Limited data are available on the performance of different automated extraction platforms and commercially available quantitative real-time PCR (QRT-PCR) methods for the quantitation of cytomegalovirus (CMV) DNA in plasma.
T3 460-992 Sentence denotes We compared the performance characteristics of the Abbott mSample preparation system DNA kit on the m24 SP instrument (Abbott), the High Pure viral nucleic acid kit on the COBAS AmpliPrep system (Roche), and the EZ1 Virus 2.0 kit on the BioRobot EZ1 extraction platform (Qiagen) coupled with the Abbott CMV PCR kit, the LightCycler CMV Quant kit (Roche), and the Q-CMV complete kit (Nanogen), for both plasma specimens from allogeneic stem cell transplant (Allo-SCT) recipients (n = 42) and the OptiQuant CMV DNA panel (AcroMetrix).
T4 993-1141 Sentence denotes The EZ1 system displayed the highest extraction efficiency over a wide range of CMV plasma DNA loads, followed by the m24 and the AmpliPrep methods.
T5 1142-1299 Sentence denotes The Nanogen PCR assay yielded higher mean CMV plasma DNA values than the Abbott and the Roche PCR assays, regardless of the platform used for DNA extraction.
T6 1300-1484 Sentence denotes Overall, the effects of the extraction method and the QRT-PCR used on CMV plasma DNA load measurements were less pronounced for specimens with high CMV DNA content (>10,000 copies/ml).
T7 1485-1656 Sentence denotes The performance characteristics of the extraction methods and QRT-PCR assays evaluated herein for clinical samples were extensible at cell-based standards from AcroMetrix.
T8 1657-1870 Sentence denotes In conclusion, different automated systems are not equally efficient for CMV DNA extraction from plasma specimens, and the plasma CMV DNA loads measured by commercially available QRT-PCRs can differ significantly.
T9 1871-2111 Sentence denotes The above findings should be taken into consideration for the establishment of cutoff values for the initiation or cessation of preemptive antiviral therapies and for the interpretation of data from clinical studies in the Allo-SCT setting.
T1 0-236 Sentence denotes Comparative evaluation of three automated systems for DNA extraction in conjunction with three commercially available real-time PCR assays for quantitation of plasma Cytomegalovirus DNAemia in allogeneic stem cell transplant recipients.
T2 237-459 Sentence denotes Limited data are available on the performance of different automated extraction platforms and commercially available quantitative real-time PCR (QRT-PCR) methods for the quantitation of cytomegalovirus (CMV) DNA in plasma.
T3 460-992 Sentence denotes We compared the performance characteristics of the Abbott mSample preparation system DNA kit on the m24 SP instrument (Abbott), the High Pure viral nucleic acid kit on the COBAS AmpliPrep system (Roche), and the EZ1 Virus 2.0 kit on the BioRobot EZ1 extraction platform (Qiagen) coupled with the Abbott CMV PCR kit, the LightCycler CMV Quant kit (Roche), and the Q-CMV complete kit (Nanogen), for both plasma specimens from allogeneic stem cell transplant (Allo-SCT) recipients (n = 42) and the OptiQuant CMV DNA panel (AcroMetrix).
T4 993-1141 Sentence denotes The EZ1 system displayed the highest extraction efficiency over a wide range of CMV plasma DNA loads, followed by the m24 and the AmpliPrep methods.
T5 1142-1299 Sentence denotes The Nanogen PCR assay yielded higher mean CMV plasma DNA values than the Abbott and the Roche PCR assays, regardless of the platform used for DNA extraction.
T6 1300-1484 Sentence denotes Overall, the effects of the extraction method and the QRT-PCR used on CMV plasma DNA load measurements were less pronounced for specimens with high CMV DNA content (>10,000 copies/ml).
T7 1485-1656 Sentence denotes The performance characteristics of the extraction methods and QRT-PCR assays evaluated herein for clinical samples were extensible at cell-based standards from AcroMetrix.
T8 1657-1870 Sentence denotes In conclusion, different automated systems are not equally efficient for CMV DNA extraction from plasma specimens, and the plasma CMV DNA loads measured by commercially available QRT-PCRs can differ significantly.
T9 1871-2111 Sentence denotes The above findings should be taken into consideration for the establishment of cutoff values for the initiation or cessation of preemptive antiviral therapies and for the interpretation of data from clinical studies in the Allo-SCT setting.