PubMed:2136386 / 474-1231 JSONTXT

{"project":"Glycan-Motif","denotations":[{"id":"T5","span":{"begin":409,"end":416},"obj":"https://glytoucan.org/Structures/Glycans/G00065MO"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T5","span":{"begin":409,"end":416},"obj":"Glycan_Motif"}],"attributes":[{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00065MO"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T12","span":{"begin":417,"end":424},"obj":"FMAID:85272"},{"id":"_T13","span":{"begin":417,"end":424},"obj":"FMAID:199625"},{"id":"_T14","span":{"begin":570,"end":580},"obj":"FMAID:166112"},{"id":"_T15","span":{"begin":573,"end":580},"obj":"FMAID:67257"},{"id":"_T16","span":{"begin":573,"end":580},"obj":"FMAID:165447"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"uniprot-human","denotations":[{"id":"T3","span":{"begin":37,"end":39},"obj":"http://www.uniprot.org/uniprot/P04264"},{"id":"T5","span":{"begin":228,"end":231},"obj":"http://www.uniprot.org/uniprot/P21941"},{"id":"T6","span":{"begin":280,"end":283},"obj":"http://www.uniprot.org/uniprot/P21941"},{"id":"T7","span":{"begin":392,"end":395},"obj":"http://www.uniprot.org/uniprot/P21941"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"uniprot-mouse","denotations":[{"id":"T3","span":{"begin":37,"end":39},"obj":"http://www.uniprot.org/uniprot/P04104"},{"id":"T4","span":{"begin":754,"end":756},"obj":"http://www.uniprot.org/uniprot/Q9DCA7"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T4","span":{"begin":102,"end":105},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T5","span":{"begin":102,"end":105},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T6","span":{"begin":132,"end":135},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T7","span":{"begin":132,"end":135},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T8","span":{"begin":163,"end":166},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T9","span":{"begin":163,"end":166},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T10","span":{"begin":220,"end":223},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T11","span":{"begin":220,"end":223},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T12","span":{"begin":232,"end":235},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T13","span":{"begin":232,"end":235},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T14","span":{"begin":284,"end":287},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T15","span":{"begin":284,"end":287},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T16","span":{"begin":327,"end":330},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T17","span":{"begin":327,"end":330},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T18","span":{"begin":387,"end":390},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T19","span":{"begin":387,"end":390},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T20","span":{"begin":396,"end":399},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T21","span":{"begin":396,"end":399},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T22","span":{"begin":523,"end":526},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T23","span":{"begin":523,"end":526},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T24","span":{"begin":613,"end":616},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T25","span":{"begin":613,"end":616},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"},{"id":"T26","span":{"begin":740,"end":743},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/208766"},{"id":"T27","span":{"begin":740,"end":743},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/172412"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T5","span":{"begin":0,"end":106},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":107,"end":236},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":237,"end":331},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":332,"end":464},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":465,"end":600},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":601,"end":659},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":660,"end":757},"obj":"Sentence"},{"id":"T5","span":{"begin":0,"end":757},"obj":"Sentence"},{"id":"T5","span":{"begin":0,"end":106},"obj":"Sentence"},{"id":"T6","span":{"begin":107,"end":236},"obj":"Sentence"},{"id":"T7","span":{"begin":237,"end":331},"obj":"Sentence"},{"id":"T8","span":{"begin":332,"end":464},"obj":"Sentence"},{"id":"T9","span":{"begin":465,"end":600},"obj":"Sentence"},{"id":"T10","span":{"begin":601,"end":659},"obj":"Sentence"},{"id":"T11","span":{"begin":660,"end":757},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"GO-BP","denotations":[{"id":"T3","span":{"begin":87,"end":98},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T4","span":{"begin":124,"end":131},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T5","span":{"begin":374,"end":383},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T8","span":{"begin":441,"end":447},"obj":"http://purl.obolibrary.org/obo/GO_0040007"},{"id":"T9","span":{"begin":636,"end":642},"obj":"http://purl.obolibrary.org/obo/GO_0040007"},{"id":"T10","span":{"begin":754,"end":756},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"GO-CC","denotations":[{"id":"T3","span":{"begin":709,"end":722},"obj":"http://purl.obolibrary.org/obo/GO_0005622"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"UBERON-AE","denotations":[{"id":"T5","span":{"begin":417,"end":424},"obj":"http://purl.obolibrary.org/obo/UBERON_0003893"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":754,"end":756},"obj":"https://acgg.asia/db/lfdb/LfDB0227"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T5","span":{"begin":417,"end":424},"obj":"http://purl.obolibrary.org/obo/UBERON_0003893"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":709,"end":722},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005622"}],"text":"The strategy was to construct E.coli K1-derived mutants with defects in activation and degradation of Sia. The inability to degrade Sia because of a defect in the Sia-specific aldolase permitted accurate quantitation of Sia and CMP-Sia. Strains EV5 and EV90 possessed a defective CMP-Sia synthetase and were unable to activate Sia. These mutants were then used to study how synthesis of Sia, CMP-Sia, and the polySia capsule was affected by growth at 15 degrees C. In contrast to wild type strains, the mutants accumulated Sia in considerable quantities (up to 100 nmol mg protein-1) at 37 degrees C. However, no Sia was detected after growth at 15 degrees C. A temperature upshift experiment showed that the intracellular concentration of Sia increased ca."}