PubMed:2136356 JSONTXT

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":47,"end":56},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":303,"end":317},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":337,"end":350},"obj":"Glycan_Motif"},{"id":"T5","span":{"begin":501,"end":514},"obj":"Glycan_Motif"},{"id":"T7","span":{"begin":614,"end":627},"obj":"Glycan_Motif"},{"id":"T9","span":{"begin":1015,"end":1028},"obj":"Glycan_Motif"},{"id":"T11","span":{"begin":1187,"end":1200},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G71832QJ"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G03986GH"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A4","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"},{"id":"A5","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A6","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"},{"id":"A7","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A8","pred":"image","subj":"T7","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"},{"id":"A9","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A10","pred":"image","subj":"T9","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"},{"id":"A11","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G58507AZ"},{"id":"A12","pred":"image","subj":"T11","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G17108EX"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":91,"end":108},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":109,"end":332},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":333,"end":473},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":474,"end":613},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":614,"end":792},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":793,"end":1014},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1015,"end":1176},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1177,"end":1274},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":108},"obj":"Sentence"},{"id":"T3","span":{"begin":109,"end":332},"obj":"Sentence"},{"id":"T4","span":{"begin":333,"end":473},"obj":"Sentence"},{"id":"T5","span":{"begin":474,"end":613},"obj":"Sentence"},{"id":"T6","span":{"begin":614,"end":792},"obj":"Sentence"},{"id":"T7","span":{"begin":793,"end":1014},"obj":"Sentence"},{"id":"T8","span":{"begin":1015,"end":1176},"obj":"Sentence"},{"id":"T9","span":{"begin":1177,"end":1274},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":47,"end":56},"obj":"https://glytoucan.org/Structures/Glycans/G71832QJ"},{"id":"T2","span":{"begin":303,"end":317},"obj":"https://glytoucan.org/Structures/Glycans/G03986GH"},{"id":"T3","span":{"begin":337,"end":350},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T4","span":{"begin":337,"end":350},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T5","span":{"begin":501,"end":514},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T6","span":{"begin":501,"end":514},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T7","span":{"begin":614,"end":627},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T8","span":{"begin":614,"end":627},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T9","span":{"begin":1015,"end":1028},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T10","span":{"begin":1015,"end":1028},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"},{"id":"T11","span":{"begin":1187,"end":1200},"obj":"https://glytoucan.org/Structures/Glycans/G17108EX"},{"id":"T12","span":{"begin":1187,"end":1200},"obj":"https://glytoucan.org/Structures/Glycans/G58507AZ"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T2","span":{"begin":35,"end":40},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T3","span":{"begin":303,"end":308},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T4","span":{"begin":303,"end":308},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T5","span":{"begin":337,"end":342},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T6","span":{"begin":337,"end":342},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T7","span":{"begin":501,"end":506},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T8","span":{"begin":501,"end":506},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T9","span":{"begin":614,"end":619},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T10","span":{"begin":614,"end":619},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T11","span":{"begin":1015,"end":1020},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T12","span":{"begin":1015,"end":1020},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T13","span":{"begin":1151,"end":1156},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T14","span":{"begin":1151,"end":1156},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"T15","span":{"begin":1187,"end":1192},"obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"T16","span":{"begin":1187,"end":1192},"obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":35,"end":48},"obj":"Glycan"},{"id":"T2","span":{"begin":337,"end":350},"obj":"Glycan"},{"id":"T3","span":{"begin":501,"end":514},"obj":"Glycan"},{"id":"T4","span":{"begin":614,"end":627},"obj":"Glycan"},{"id":"T5","span":{"begin":1015,"end":1028},"obj":"Glycan"},{"id":"T6","span":{"begin":1187,"end":1200},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A7","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A8","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A9","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A4","pred":"glycosmos_id","subj":"T4","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A10","pred":"image","subj":"T4","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A5","pred":"glycosmos_id","subj":"T5","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A11","pred":"image","subj":"T5","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A6","pred":"glycosmos_id","subj":"T6","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A12","pred":"image","subj":"T6","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":614,"end":627},"obj":"Glycan"},{"id":"T2","span":{"begin":1015,"end":1028},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A3","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G00066MO"},{"id":"A4","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G00066MO"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-CL","denotations":[{"id":"T1","span":{"begin":1237,"end":1241},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000560"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"Body_part"},{"id":"T2","span":{"begin":303,"end":308},"obj":"Body_part"},{"id":"T3","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T4","span":{"begin":501,"end":506},"obj":"Body_part"},{"id":"T5","span":{"begin":614,"end":619},"obj":"Body_part"},{"id":"T6","span":{"begin":1015,"end":1020},"obj":"Body_part"},{"id":"T7","span":{"begin":1151,"end":1156},"obj":"Body_part"},{"id":"T8","span":{"begin":1187,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-Taxon","denotations":[{"id":"T1","span":{"begin":71,"end":76},"obj":"Organism"},{"id":"T2","span":{"begin":233,"end":238},"obj":"Organism"},{"id":"T3","span":{"begin":273,"end":278},"obj":"Organism"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":90},"obj":"Sentence"},{"id":"T2","span":{"begin":91,"end":108},"obj":"Sentence"},{"id":"T3","span":{"begin":109,"end":332},"obj":"Sentence"},{"id":"T4","span":{"begin":333,"end":473},"obj":"Sentence"},{"id":"T5","span":{"begin":474,"end":613},"obj":"Sentence"},{"id":"T6","span":{"begin":614,"end":792},"obj":"Sentence"},{"id":"T7","span":{"begin":793,"end":1014},"obj":"Sentence"},{"id":"T8","span":{"begin":1015,"end":1176},"obj":"Sentence"},{"id":"T9","span":{"begin":1177,"end":1274},"obj":"Sentence"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-FMA","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"Body_part"},{"id":"T2","span":{"begin":77,"end":89},"obj":"Body_part"},{"id":"T3","span":{"begin":239,"end":251},"obj":"Body_part"},{"id":"T4","span":{"begin":303,"end":308},"obj":"Body_part"},{"id":"T5","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T6","span":{"begin":501,"end":506},"obj":"Body_part"},{"id":"T7","span":{"begin":614,"end":619},"obj":"Body_part"},{"id":"T8","span":{"begin":677,"end":689},"obj":"Body_part"},{"id":"T9","span":{"begin":885,"end":897},"obj":"Body_part"},{"id":"T10","span":{"begin":1001,"end":1013},"obj":"Body_part"},{"id":"T11","span":{"begin":1015,"end":1020},"obj":"Body_part"},{"id":"T12","span":{"begin":1071,"end":1083},"obj":"Body_part"},{"id":"T13","span":{"begin":1151,"end":1156},"obj":"Body_part"},{"id":"T14","span":{"begin":1187,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"FMA:9670"},{"id":"A2","pred":"db_id","subj":"T2","obj":"FMA:62851"},{"id":"A3","pred":"db_id","subj":"T3","obj":"FMA:62851"},{"id":"A4","pred":"db_id","subj":"T4","obj":"FMA:9670"},{"id":"A5","pred":"db_id","subj":"T5","obj":"FMA:9670"},{"id":"A6","pred":"db_id","subj":"T6","obj":"FMA:9670"},{"id":"A7","pred":"db_id","subj":"T7","obj":"FMA:9670"},{"id":"A8","pred":"db_id","subj":"T8","obj":"FMA:62851"},{"id":"A9","pred":"db_id","subj":"T9","obj":"FMA:62851"},{"id":"A10","pred":"db_id","subj":"T10","obj":"FMA:62851"},{"id":"A11","pred":"db_id","subj":"T11","obj":"FMA:9670"},{"id":"A12","pred":"db_id","subj":"T12","obj":"FMA:62851"},{"id":"A13","pred":"db_id","subj":"T13","obj":"FMA:9670"},{"id":"A14","pred":"db_id","subj":"T14","obj":"FMA:9670"}],"namespaces":[{"prefix":"FMA","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"Body_part"},{"id":"T2","span":{"begin":303,"end":308},"obj":"Body_part"},{"id":"T3","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T4","span":{"begin":501,"end":506},"obj":"Body_part"},{"id":"T5","span":{"begin":614,"end":619},"obj":"Body_part"},{"id":"T6","span":{"begin":1015,"end":1020},"obj":"Body_part"},{"id":"T7","span":{"begin":1151,"end":1156},"obj":"Body_part"},{"id":"T8","span":{"begin":1187,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A4","pred":"mat_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A6","pred":"mat_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A7","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A8","pred":"mat_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":71,"end":76},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":233,"end":238},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":273,"end":278},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"9606"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9606"},{"id":"A3","pred":"db_id","subj":"T3","obj":"9606"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"Body_part"},{"id":"T2","span":{"begin":303,"end":308},"obj":"Body_part"},{"id":"T3","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T4","span":{"begin":416,"end":421},"obj":"Body_part"},{"id":"T6","span":{"begin":501,"end":506},"obj":"Body_part"},{"id":"T7","span":{"begin":614,"end":619},"obj":"Body_part"},{"id":"T8","span":{"begin":1015,"end":1020},"obj":"Body_part"},{"id":"T9","span":{"begin":1151,"end":1156},"obj":"Body_part"},{"id":"T10","span":{"begin":1187,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000119"},{"id":"A5","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0022303"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A9","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"A10","pred":"uberon_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":35,"end":40},"obj":"Body_part"},{"id":"T3","span":{"begin":303,"end":308},"obj":"Body_part"},{"id":"T5","span":{"begin":337,"end":342},"obj":"Body_part"},{"id":"T7","span":{"begin":501,"end":506},"obj":"Body_part"},{"id":"T9","span":{"begin":614,"end":619},"obj":"Body_part"},{"id":"T11","span":{"begin":1015,"end":1020},"obj":"Body_part"},{"id":"T13","span":{"begin":1151,"end":1156},"obj":"Body_part"},{"id":"T15","span":{"begin":1187,"end":1192},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A2","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A3","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A4","pred":"mat_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A5","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A6","pred":"mat_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A7","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A8","pred":"mat_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A9","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A10","pred":"mat_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A11","pred":"mat_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A12","pred":"mat_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A13","pred":"mat_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A14","pred":"mat_id","subj":"T13","obj":"http://purl.obolibrary.org/obo/MAT_0000315"},{"id":"A15","pred":"mat_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/MAT_0000083"},{"id":"A16","pred":"mat_id","subj":"T15","obj":"http://purl.obolibrary.org/obo/MAT_0000315"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":1237,"end":1241},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000560"}],"text":"Glycolipid- and glycoprotein-based blood group A antigen expression in human thrombocytes. A1/A2 difference.\nTotal non-acid glycolipid fractions and total sodium dodecylsulphate (SDS) solubilized protein fractions were isolated from human thrombocytes obtained from single human donors having different blood group A1/A2 phenotypes. The blood group A glycolipid antigens were characterized by immunostaining of thin layer plates with different monoclonal anti-A antibodies. The glycoproteins carrying blood group A epitopes were identified by SDS-PAGE and Western blot analysis using a monoclonal anti-A antibody. Blood group A glycolipid antigens were found in both A1 and A2 thrombocytes but the A2 individuals expressed at least ten times less A glycolipids compared to the A1 individuals. Expression of A type 3/4 chain and small amounts of A type 1 chain glycolipids were seen in thrombocytes of both A1 and A2 individuals, while the type 2 chain A glycolipids appeared to be missing from the A2 thrombocytes. Blood group A reactive glycoproteins were only found in thrombocytes of A1 individuals and could not be detected in A2 individuals or a blood group O individual. The major blood group A glycoprotein were found as a double band migrating in the 130 kDa region."}