PubMed:21189159 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/21189159","sourcedb":"PubMed","sourceid":"21189159","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/21189159","text":"Rapid functional screening of effective siRNAs against Plk1 and its growth inhibitory effects in laryngeal carcinoma cells.\nPlk 1 is overexpressed in many human malignancies including laryngeal carcinoma. However, its therapeutic potential has been never examined in laryngeal carcinoma. In the present study, a simple cellular morphology-based strategy was firstly proposed for rapidly screening the effective siRNAs against Plk1. Furthermore, we investigated the effects of Plk1 depletion via a novel identified effective siRNA against Plk1, Plk1 siRNA-607, on human laryngeal carcinoma Hep-2 cells. The results indicated that Plk1 siRNA-607 transfection resulted in a significant inhibition in Plk1 expression in cells, and subsequently caused a dramatic mitotic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of the laryngeal carcinoma cells. Taken together, our present study not only suggests a simple strategy for rapidly screening effective siRNAs against Plk1 but also implicates that Plk1 may serve as a potential therapeutic target in human laryngeal carcinoma.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":184,"end":203},"obj":"HP_0012118"},{"id":"T2","span":{"begin":267,"end":286},"obj":"HP_0012118"},{"id":"T3","span":{"begin":569,"end":588},"obj":"HP_0012118"},{"id":"T4","span":{"begin":907,"end":926},"obj":"HP_0012118"},{"id":"T5","span":{"begin":1139,"end":1158},"obj":"HP_0012118"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"},{"subj":"T3","pred":"source","obj":"PubmedHPO"},{"subj":"T4","pred":"source","obj":"PubmedHPO"},{"subj":"T5","pred":"source","obj":"PubmedHPO"}]},{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":697,"end":701},"obj":"gene:5347"},{"id":"T1","span":{"begin":907,"end":926},"obj":"disease:C0595989"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"attributes":[{"subj":"T0","pred":"source","obj":"DisGeNET"},{"subj":"T1","pred":"source","obj":"DisGeNET"}]},{"project":"DisGeNET5_gene_disease","denotations":[{"id":"21189159-0#55#59#gene5347","span":{"begin":55,"end":59},"obj":"gene5347"},{"id":"21189159-0#97#116#diseaseC0595989","span":{"begin":97,"end":116},"obj":"diseaseC0595989"}],"relations":[{"id":"55#59#gene534797#116#diseaseC0595989","pred":"associated_with","subj":"21189159-0#55#59#gene5347","obj":"21189159-0#97#116#diseaseC0595989"}],"attributes":[{"subj":"21189159-0#55#59#gene5347","pred":"source","obj":"DisGeNET5_gene_disease"},{"subj":"21189159-0#97#116#diseaseC0595989","pred":"source","obj":"DisGeNET5_gene_disease"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#ecd593","default":true},{"id":"DisGeNET","color":"#bb93ec"},{"id":"DisGeNET5_gene_disease","color":"#93eca1"}]}]}}