Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-162 |
Sentence |
denotes |
A transcriptional enhancer essential for the expression of the rat cholecystokinin gene contains a sequence identical to the -296 element of the human c-fos gene. |
T2 |
163-309 |
Sentence |
denotes |
We report the identification and characterization of the cis-acting elements responsible for the expression of the rat cholecystokinin (CCK) gene. |
T3 |
310-481 |
Sentence |
denotes |
Deletion mutations were constructed by linking variable amounts of the 5'-flanking region of the CCK gene to the bacterial chloramphenicol acetyltransferase reporter gene. |
T4 |
482-658 |
Sentence |
denotes |
The transcriptional activity of the CCK promoter deletion constructs was measured by monitoring chloramphenicol acetyltransferase enzyme activity after transient transfections. |
T5 |
659-775 |
Sentence |
denotes |
It is shown that sequences within 102 base pairs of the cap site are required for the expression from this promoter. |
T6 |
776-1017 |
Sentence |
denotes |
This region contains a sequence that is identical to the -296 element of the human c-fos gene and is homologous with the polyoma enhancer and the cAMP- and 12-O-tetradecanoylphorbol-13-acetate-responsive elements described for several genes. |
T7 |
1018-1253 |
Sentence |
denotes |
In addition, the -119 to -81 fragment of the CCK promoter contains a transcriptional enhancer that potentiates the transcription from the herpes simplex virus thymidine kinase promoter in a position- and orientation-independent manner. |
T8 |
1254-1437 |
Sentence |
denotes |
DNase I protection and gel retardation experiments indicated the ability of several trans-acting factors found in nuclear extracts to bind specifically to regions of the CCK promoter. |
T9 |
1438-1510 |
Sentence |
denotes |
In particular, two complexes formed adjacent to the CCK enhancer region. |
T10 |
1511-1689 |
Sentence |
denotes |
One complex, CCK-1a, formed with sequences 5' to the enhancer whereas the other complex, CCK-1b, formed with the sequences identified by DNase I footprinting, 3' to the enhancer. |
T11 |
1690-1849 |
Sentence |
denotes |
Oligonucleotide competition experiments indicated that these complexes are formed by the same transacting factor or factors with similar binding specificities. |
T1 |
0-162 |
Sentence |
denotes |
A transcriptional enhancer essential for the expression of the rat cholecystokinin gene contains a sequence identical to the -296 element of the human c-fos gene. |
T2 |
163-309 |
Sentence |
denotes |
We report the identification and characterization of the cis-acting elements responsible for the expression of the rat cholecystokinin (CCK) gene. |
T3 |
310-481 |
Sentence |
denotes |
Deletion mutations were constructed by linking variable amounts of the 5'-flanking region of the CCK gene to the bacterial chloramphenicol acetyltransferase reporter gene. |
T4 |
482-658 |
Sentence |
denotes |
The transcriptional activity of the CCK promoter deletion constructs was measured by monitoring chloramphenicol acetyltransferase enzyme activity after transient transfections. |
T5 |
659-775 |
Sentence |
denotes |
It is shown that sequences within 102 base pairs of the cap site are required for the expression from this promoter. |
T6 |
776-1017 |
Sentence |
denotes |
This region contains a sequence that is identical to the -296 element of the human c-fos gene and is homologous with the polyoma enhancer and the cAMP- and 12-O-tetradecanoylphorbol-13-acetate-responsive elements described for several genes. |
T7 |
1018-1253 |
Sentence |
denotes |
In addition, the -119 to -81 fragment of the CCK promoter contains a transcriptional enhancer that potentiates the transcription from the herpes simplex virus thymidine kinase promoter in a position- and orientation-independent manner. |
T8 |
1254-1437 |
Sentence |
denotes |
DNase I protection and gel retardation experiments indicated the ability of several trans-acting factors found in nuclear extracts to bind specifically to regions of the CCK promoter. |
T9 |
1438-1510 |
Sentence |
denotes |
In particular, two complexes formed adjacent to the CCK enhancer region. |
T10 |
1511-1689 |
Sentence |
denotes |
One complex, CCK-1a, formed with sequences 5' to the enhancer whereas the other complex, CCK-1b, formed with the sequences identified by DNase I footprinting, 3' to the enhancer. |
T11 |
1690-1849 |
Sentence |
denotes |
Oligonucleotide competition experiments indicated that these complexes are formed by the same transacting factor or factors with similar binding specificities. |