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PubMed:21129193 JSONTXT

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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 139-564 BACKGROUND denotes A novel series of methylene-substituted DIMs (C-DIMs), namely 1,1-bis(3'-indolyl)-1-(p-substituted phenyl)methanes containing t-butyl (DIM-C-pPhtBu) and phenyl (DIM-C-pPhC6H5) groups inhibit proliferation of invasive estrogen receptor-negative MDA-MB-231 and MDA-MB-453 human breast cancer cell lines with IC50 values between 1-5 uM. The main purpose of this study was to investigate the pathways of C-DIM-induced cell death.
T2 574-841 METHODS denotes The effects of the C-DIMs on apoptotic, necrotic and autophagic cell death were determined using caspase inhibitors, measurement of lactate dehydrogenase release, and several markers of autophagy including Beclin and light chain associated protein 3 expression (LC3).
T3 851-1559 RESULTS denotes The C-DIM compounds did not induce apoptosis and only DIM-C-pPhCF3 exhibited necrotic effects. However, treatment of MDA-MB-231 and MDA-MB-453 cells with C-DIMs resulted in accumulation of LC3-II compared to LC3-I protein, a characteristic marker of autophagy, and transient transfection of green fluorescent protein-LC3 also revealed that treatment with C-DIMs induced a redistribution of LC3 to autophagosomes after C-DIM treatment. In addition, the autofluorescent drug monodansylcadaverine (MDC), a specific autophagolysosome marker, accumulated in vacuoles after C-DIM treatment, and western blot analysis of lysates from cells treated with C-DIMs showed that the Beclin 1/Bcl-2 protein ratio increased.
T4 1572-1740 CONCLUSIONS denotes The results suggest that C-DIM compounds may represent a new mechanism-based agent for treating drug-resistant ER-negative breast tumors through induction of autophagy.

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-126 Sentence denotes 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes induce autophagic cell death in estrogen receptor negative breast cancer.
TextSentencer_T2 127-138 Sentence denotes BACKGROUND:
TextSentencer_T3 139-472 Sentence denotes A novel series of methylene-substituted DIMs (C-DIMs), namely 1,1-bis(3'-indolyl)-1-(p-substituted phenyl)methanes containing t-butyl (DIM-C-pPhtBu) and phenyl (DIM-C-pPhC6H5) groups inhibit proliferation of invasive estrogen receptor-negative MDA-MB-231 and MDA-MB-453 human breast cancer cell lines with IC50 values between 1-5 uM.
TextSentencer_T4 473-564 Sentence denotes The main purpose of this study was to investigate the pathways of C-DIM-induced cell death.
TextSentencer_T5 565-573 Sentence denotes METHODS:
TextSentencer_T6 574-841 Sentence denotes The effects of the C-DIMs on apoptotic, necrotic and autophagic cell death were determined using caspase inhibitors, measurement of lactate dehydrogenase release, and several markers of autophagy including Beclin and light chain associated protein 3 expression (LC3).
TextSentencer_T7 842-850 Sentence denotes RESULTS:
TextSentencer_T8 851-945 Sentence denotes The C-DIM compounds did not induce apoptosis and only DIM-C-pPhCF3 exhibited necrotic effects.
TextSentencer_T9 946-1285 Sentence denotes However, treatment of MDA-MB-231 and MDA-MB-453 cells with C-DIMs resulted in accumulation of LC3-II compared to LC3-I protein, a characteristic marker of autophagy, and transient transfection of green fluorescent protein-LC3 also revealed that treatment with C-DIMs induced a redistribution of LC3 to autophagosomes after C-DIM treatment.
TextSentencer_T10 1286-1559 Sentence denotes In addition, the autofluorescent drug monodansylcadaverine (MDC), a specific autophagolysosome marker, accumulated in vacuoles after C-DIM treatment, and western blot analysis of lysates from cells treated with C-DIMs showed that the Beclin 1/Bcl-2 protein ratio increased.
TextSentencer_T11 1560-1571 Sentence denotes CONCLUSION:
TextSentencer_T12 1572-1740 Sentence denotes The results suggest that C-DIM compounds may represent a new mechanism-based agent for treating drug-resistant ER-negative breast tumors through induction of autophagy.
T1 0-126 Sentence denotes 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes induce autophagic cell death in estrogen receptor negative breast cancer.
T2 127-138 Sentence denotes BACKGROUND:
T3 139-472 Sentence denotes A novel series of methylene-substituted DIMs (C-DIMs), namely 1,1-bis(3'-indolyl)-1-(p-substituted phenyl)methanes containing t-butyl (DIM-C-pPhtBu) and phenyl (DIM-C-pPhC6H5) groups inhibit proliferation of invasive estrogen receptor-negative MDA-MB-231 and MDA-MB-453 human breast cancer cell lines with IC50 values between 1-5 uM.
T4 473-564 Sentence denotes The main purpose of this study was to investigate the pathways of C-DIM-induced cell death.
T5 565-573 Sentence denotes METHODS:
T6 574-841 Sentence denotes The effects of the C-DIMs on apoptotic, necrotic and autophagic cell death were determined using caspase inhibitors, measurement of lactate dehydrogenase release, and several markers of autophagy including Beclin and light chain associated protein 3 expression (LC3).
T7 842-850 Sentence denotes RESULTS:
T8 851-945 Sentence denotes The C-DIM compounds did not induce apoptosis and only DIM-C-pPhCF3 exhibited necrotic effects.
T9 946-1285 Sentence denotes However, treatment of MDA-MB-231 and MDA-MB-453 cells with C-DIMs resulted in accumulation of LC3-II compared to LC3-I protein, a characteristic marker of autophagy, and transient transfection of green fluorescent protein-LC3 also revealed that treatment with C-DIMs induced a redistribution of LC3 to autophagosomes after C-DIM treatment.
T10 1286-1559 Sentence denotes In addition, the autofluorescent drug monodansylcadaverine (MDC), a specific autophagolysosome marker, accumulated in vacuoles after C-DIM treatment, and western blot analysis of lysates from cells treated with C-DIMs showed that the Beclin 1/Bcl-2 protein ratio increased.
T11 1560-1571 Sentence denotes CONCLUSION:
T12 1572-1740 Sentence denotes The results suggest that C-DIM compounds may represent a new mechanism-based agent for treating drug-resistant ER-negative breast tumors through induction of autophagy.

DisGeNET

Id Subject Object Predicate Lexical cue
T0 85-102 gene:2099 denotes estrogen receptor
T1 112-125 disease:C0678222 denotes breast cancer
T2 85-102 gene:2099 denotes estrogen receptor
T3 112-125 disease:C0006142 denotes breast cancer
T4 356-373 gene:2099 denotes estrogen receptor
T5 415-428 disease:C0678222 denotes breast cancer
T6 356-373 gene:2099 denotes estrogen receptor
T7 415-428 disease:C0006142 denotes breast cancer
R1 T0 T1 associated_with estrogen receptor,breast cancer
R2 T2 T3 associated_with estrogen receptor,breast cancer
R3 T4 T5 associated_with estrogen receptor,breast cancer
R4 T6 T7 associated_with estrogen receptor,breast cancer

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 415-428 HP_0003002 denotes breast cancer
T2 415-428 HP_0100013 denotes breast cancer
T3 422-428 HP_0002664 denotes cancer

Allie

Id Subject Object Predicate Lexical cue
SS1_21129193_5_0 791-834 expanded denotes light chain associated protein 3 expression
SS2_21129193_5_0 836-839 abbr denotes LC3
SS1_21129193_9_0 1324-1344 expanded denotes monodansylcadaverine
SS2_21129193_9_0 1346-1349 abbr denotes MDC
AE1_21129193_5_0 SS1_21129193_5_0 SS2_21129193_5_0 abbreviatedTo light chain associated protein 3 expression,LC3
AE1_21129193_9_0 SS1_21129193_9_0 SS2_21129193_9_0 abbreviatedTo monodansylcadaverine,MDC

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
21129193-0#85#102#gene2099 85-102 gene2099 denotes estrogen receptor
21129193-0#112#125#diseaseC0006142 112-125 diseaseC0006142 denotes breast cancer
21129193-0#112#125#diseaseC0678222 112-125 diseaseC0678222 denotes breast cancer
85#102#gene2099112#125#diseaseC0006142 21129193-0#85#102#gene2099 21129193-0#112#125#diseaseC0006142 associated_with estrogen receptor,breast cancer
85#102#gene2099112#125#diseaseC0678222 21129193-0#85#102#gene2099 21129193-0#112#125#diseaseC0678222 associated_with estrogen receptor,breast cancer

DisGeNet-2017-sample

Id Subject Object Predicate Lexical cue
T346 85-102 gene:2099 denotes estrogen receptor
T347 112-125 disease:C0006142 denotes breast cancer
R1 T346 T347 associated_with estrogen receptor,breast cancer
R2 T346 T347 associated_with estrogen receptor,breast cancer

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 112-118 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T2 415-421 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T3 1695-1701 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 112-118 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T2 415-421 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T3 1695-1701 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast