PubMed:2108863 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/2108863","sourcedb":"PubMed","sourceid":"2108863","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/2108863","text":"Synergism in ternary complex formation between the dimeric glycoprotein p67SRF, polypeptide p62TCF and the c-fos serum response element.\nTranscriptional regulation of the c-fos proto-oncogene requires the serum response element (SRE) which is complexed by a multi-protein assembly observed both in vitro and in vivo. Two protein factors, p67SRF and p62TCF (previously called p62), are required to interact with the SRE for efficient induction of c-fos by serum. By quantitative band shift electrophoresis we measure at least a 50-fold increase in SRE affinity for p67SRF/p62TCF over p67SRF alone. Stoichiometrically we determine that the ternary complex with p62TCF involves p67SRF in dimeric form. We demonstrate that p67SRF is a glycosylated nuclear transcription factor carrying terminal N-acetylglucosamine (GlcNAc) as a post-translational modification. A proteolytic limit digestion product, approximately 13 kd in size, was generated from the p67SRF-SRE complex. This p67SRF-core domain binds SRE, can dimerize with p67SRF and is still able to form a ternary complex with p62TCF. Therefore, three functional activities can be ascribed to this small p67SRF-core domain: specific DNA binding, dimerization and interaction with p62TCF. We demonstrate that these functions map within the p67SRF core fragment containing the region between amino acids 93 and 222.","tracks":[{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":72,"end":78},"obj":"Protein"},{"id":"T2","span":{"begin":92,"end":98},"obj":"Protein"},{"id":"T3","span":{"begin":107,"end":112},"obj":"Protein"},{"id":"T4","span":{"begin":171,"end":176},"obj":"Protein"},{"id":"T5","span":{"begin":338,"end":344},"obj":"Protein"},{"id":"T6","span":{"begin":349,"end":355},"obj":"Protein"},{"id":"T7","span":{"begin":375,"end":378},"obj":"Protein"},{"id":"T8","span":{"begin":446,"end":451},"obj":"Protein"},{"id":"T9","span":{"begin":564,"end":570},"obj":"Protein"},{"id":"T10","span":{"begin":571,"end":577},"obj":"Protein"},{"id":"T11","span":{"begin":583,"end":589},"obj":"Protein"},{"id":"T12","span":{"begin":659,"end":665},"obj":"Protein"},{"id":"T13","span":{"begin":675,"end":681},"obj":"Protein"},{"id":"T14","span":{"begin":719,"end":725},"obj":"Protein"},{"id":"T15","span":{"begin":949,"end":955},"obj":"Protein"},{"id":"T16","span":{"begin":974,"end":980},"obj":"Protein"},{"id":"T17","span":{"begin":1022,"end":1028},"obj":"Protein"},{"id":"T18","span":{"begin":1078,"end":1084},"obj":"Protein"},{"id":"T19","span":{"begin":1155,"end":1161},"obj":"Protein"},{"id":"T20","span":{"begin":1231,"end":1237},"obj":"Protein"},{"id":"T21","span":{"begin":1290,"end":1296},"obj":"Protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T11","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T12","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T13","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T14","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T15","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T16","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T17","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T18","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T19","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T20","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T21","pred":"source","obj":"bionlp-st-epi-2011-training"}]},{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":791,"end":810},"obj":"chem:24139"},{"id":"PD-NGLY1-deficiency-B_T2","span":{"begin":812,"end":818},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"attributes":[{"subj":"PD-NGLY1-deficiency-B_T1","pred":"source","obj":"NGLY1-deficiency"},{"subj":"PD-NGLY1-deficiency-B_T2","pred":"source","obj":"NGLY1-deficiency"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"bionlp-st-epi-2011-training","color":"#93ecc4","default":true},{"id":"NGLY1-deficiency","color":"#ecaa93"}]}]}}