PubMed:21079938 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/21079938","sourcedb":"PubMed","sourceid":"21079938","text":"Formation of macromolecule complex with Bacillus thuringiensis Cry1A toxins and chlorophyllide binding 252-kDa lipocalin-like protein locating on Bombyx mori midgut membrane.\nP252, a 252-kDa Bombyx mori protein located on the larval midgut membrane, has been shown to bind strongly with Bacillus thuringiensis Cry1A toxins (Hossain et al. Appl Environ Microbiol 70:4604-4612, 2004). P252 was also shown to bind chlorophyllide (Chlide) to form red fluorescence-emitting complex Bm252RFP with significant antimicrobial activity (Pandian et al. Appl Environ Microbiol 74:1324-1331, 2008). In this article, we show that Cry1A toxin bound with Bm252RFP and Bm252RFP-Cry1A macrocomplex, with both antimicrobial and insecticidal activities, was formed. The insecticidal activity of Bm252RFP-Cry1Ab was reduced from an LD₅₀ of 1.62 to 5.05 μg, but Bm252RFP-Cry1Aa and Bm252RFP-Cry1Ac did not show such reduction. On the other hand, the antimicrobial activity of Bm252RFP-Cry1Ab was shown to retain almost the same activity as Bm252RFP, while the other two complexes lost around 30% activity. The intensity of photo absorbance and fluorescence emission of Bm252RFP-Cry1Ab were significantly reduced compared to those of the other two complexes. Circular dichroism showed that the contents of Cry1Ab α-helix was significantly decreased in Bm252RFP-Cry1Ab but not in the other two toxins. These data suggested that the reduction of contents of α-helix in Cry1Ab affected the insecticidal activity of the macrocomplex but did not alter the antimicrobial moiety in the macrocomplex of Bm252RFP-Cry1Ab.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":40,"end":62},"obj":"Species:1428"},{"id":"T2","span":{"begin":80,"end":94},"obj":"Chemical:MESH:D002735"},{"id":"T3","span":{"begin":146,"end":157},"obj":"Species:7091"},{"id":"T4","span":{"begin":191,"end":202},"obj":"Species:7091"},{"id":"T5","span":{"begin":287,"end":309},"obj":"Species:1428"},{"id":"T6","span":{"begin":411,"end":425},"obj":"Chemical:MESH:D002735"},{"id":"T7","span":{"begin":427,"end":433},"obj":"Chemical:MESH:D002735"},{"id":"T8","span":{"begin":1283,"end":1287},"obj":"Gene:100500923"},{"id":"T9","span":{"begin":1444,"end":1448},"obj":"Gene:100500923"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":40,"end":62},"obj":"Species:1428"},{"id":"T2","span":{"begin":80,"end":94},"obj":"Chemical:MESH:D002735"},{"id":"T3","span":{"begin":146,"end":157},"obj":"Species:7091"},{"id":"T4","span":{"begin":191,"end":202},"obj":"Species:7091"},{"id":"T5","span":{"begin":287,"end":309},"obj":"Species:1428"},{"id":"T6","span":{"begin":411,"end":425},"obj":"Chemical:MESH:D002735"},{"id":"T7","span":{"begin":427,"end":433},"obj":"Chemical:MESH:D002735"},{"id":"T8","span":{"begin":1283,"end":1287},"obj":"Gene:100500923"},{"id":"T9","span":{"begin":1444,"end":1448},"obj":"Gene:100500923"},{"id":"T16","span":{"begin":784,"end":790},"obj":"BMO_00500"},{"id":"T18","span":{"begin":963,"end":969},"obj":"BMO_00500"},{"id":"T19","span":{"begin":1156,"end":1162},"obj":"BMO_00500"},{"id":"T20","span":{"begin":1283,"end":1289},"obj":"BMO_00500"},{"id":"T21","span":{"begin":1338,"end":1344},"obj":"BMO_00500"},{"id":"T22","span":{"begin":1444,"end":1450},"obj":"BMO_00500"},{"id":"T23","span":{"begin":1581,"end":1587},"obj":"BMO_00500"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"},{"subj":"T16","pred":"source","obj":"silkwormbase"},{"subj":"T18","pred":"source","obj":"silkwormbase"},{"subj":"T19","pred":"source","obj":"silkwormbase"},{"subj":"T20","pred":"source","obj":"silkwormbase"},{"subj":"T21","pred":"source","obj":"silkwormbase"},{"subj":"T22","pred":"source","obj":"silkwormbase"},{"subj":"T23","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm_phenotype","denotations":[{"id":"T23","span":{"begin":1581,"end":1587},"obj":"BMO_00500"},{"id":"T22","span":{"begin":1444,"end":1450},"obj":"BMO_00500"},{"id":"T21","span":{"begin":1338,"end":1344},"obj":"BMO_00500"},{"id":"T20","span":{"begin":1283,"end":1289},"obj":"BMO_00500"},{"id":"T19","span":{"begin":1156,"end":1162},"obj":"BMO_00500"},{"id":"T18","span":{"begin":963,"end":969},"obj":"BMO_00500"},{"id":"T16","span":{"begin":784,"end":790},"obj":"BMO_00500"},{"id":"T9","span":{"begin":1444,"end":1448},"obj":"Gene:100500923"},{"id":"T8","span":{"begin":1283,"end":1287},"obj":"Gene:100500923"},{"id":"T7","span":{"begin":427,"end":433},"obj":"Chemical:MESH:D002735"},{"id":"T6","span":{"begin":411,"end":425},"obj":"Chemical:MESH:D002735"},{"id":"T5","span":{"begin":287,"end":309},"obj":"Species:1428"},{"id":"T4","span":{"begin":191,"end":202},"obj":"Species:7091"},{"id":"T3","span":{"begin":146,"end":157},"obj":"Species:7091"},{"id":"T2","span":{"begin":80,"end":94},"obj":"Chemical:MESH:D002735"},{"id":"T1","span":{"begin":40,"end":62},"obj":"Species:1428"}],"attributes":[{"subj":"T23","pred":"source","obj":"silkworm_phenotype"},{"subj":"T22","pred":"source","obj":"silkworm_phenotype"},{"subj":"T21","pred":"source","obj":"silkworm_phenotype"},{"subj":"T20","pred":"source","obj":"silkworm_phenotype"},{"subj":"T19","pred":"source","obj":"silkworm_phenotype"},{"subj":"T18","pred":"source","obj":"silkworm_phenotype"},{"subj":"T16","pred":"source","obj":"silkworm_phenotype"},{"subj":"T9","pred":"source","obj":"silkworm_phenotype"},{"subj":"T8","pred":"source","obj":"silkworm_phenotype"},{"subj":"T7","pred":"source","obj":"silkworm_phenotype"},{"subj":"T6","pred":"source","obj":"silkworm_phenotype"},{"subj":"T5","pred":"source","obj":"silkworm_phenotype"},{"subj":"T4","pred":"source","obj":"silkworm_phenotype"},{"subj":"T3","pred":"source","obj":"silkworm_phenotype"},{"subj":"T2","pred":"source","obj":"si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