PubMed:20962077 JSONTXT

{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":760,"end":776},"obj":"HP_0002721"}],"text":"Simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with O-linked carbohydrate.\nAlthough stretches of serine and threonine are sometimes sites for O-linked carbohydrate attachment, specific sequence and structural determinants for O-linked attachment remain ill defined. The gp120 envelope protein of SIVmac239 contains a serine-threonine-rich stretch of amino acids at positions 128 to 139. Here we show that lectin protein from jackfruit seed (jacalin), which binds to non- and monosialylated core 1 O-linked carbohydrate, potently inhibited the replication of SIVmac239. Selection of a jacalin-resistant SIVmac239 variant population resulted in virus with specific substitutions within amino acids 128 to 139. Cloned simian immunodeficiency virus (SIV) variants with substitutions in the 128-to-139 region had infectivities equivalent to, or within 1 log unit of, that of SIVmac239 and were resistant to the inhibitory effects of jacalin. Characterization of the SIVmac239 gp120 O-linked glycome showed the presence of core 1 and core 2 O-linked carbohydrate; a 128-to-139-substituted variant gp120 from jacalin-resistant SIV lacked O-linked carbohydrate. Unlike that of SIVmac239, the replication of HIV-1 strain NL4-3 was resistant to inhibition by jacalin. Purified gp120s from four SIVmac and SIVsm strains bound jacalin strongly in an enzyme-linked immunosorbent assay, while nine different HIV-1 gp120s, two SIVcpz gp120s, and 128-to-139-substituted SIVmac239 gp120 did not bind jacalin. The ability or inability to bind jacalin thus correlated with the presence of the serine-threonine-rich stretch in the SIVmac and SIVsm gp120s and the absence of such stretches in the SIVcpz and HIV-1 gp120s. Consistent with sequence predictions, two HIV-2 gp120s bound jacalin, while one did not. These data demonstrate the presence of non- and monosialylated core 1 O-linked carbohydrate on the gp120s of SIVmac and SIVsm and the lack of these modifications on HIV-1 and SIVcpz gp120s."}

{"project":"Allie","denotations":[{"id":"SS1_20962077_5_0","span":{"begin":753,"end":782},"obj":"expanded"},{"id":"SS2_20962077_5_0","span":{"begin":784,"end":787},"obj":"abbr"}],"relations":[{"id":"AE1_20962077_5_0","pred":"abbreviatedTo","subj":"SS1_20962077_5_0","obj":"SS2_20962077_5_0"}],"text":"Simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with O-linked carbohydrate.\nAlthough stretches of serine and threonine are sometimes sites for O-linked carbohydrate attachment, specific sequence and structural determinants for O-linked attachment remain ill defined. The gp120 envelope protein of SIVmac239 contains a serine-threonine-rich stretch of amino acids at positions 128 to 139. Here we show that lectin protein from jackfruit seed (jacalin), which binds to non- and monosialylated core 1 O-linked carbohydrate, potently inhibited the replication of SIVmac239. Selection of a jacalin-resistant SIVmac239 variant population resulted in virus with specific substitutions within amino acids 128 to 139. Cloned simian immunodeficiency virus (SIV) variants with substitutions in the 128-to-139 region had infectivities equivalent to, or within 1 log unit of, that of SIVmac239 and were resistant to the inhibitory effects of jacalin. Characterization of the SIVmac239 gp120 O-linked glycome showed the presence of core 1 and core 2 O-linked carbohydrate; a 128-to-139-substituted variant gp120 from jacalin-resistant SIV lacked O-linked carbohydrate. Unlike that of SIVmac239, the replication of HIV-1 strain NL4-3 was resistant to inhibition by jacalin. Purified gp120s from four SIVmac and SIVsm strains bound jacalin strongly in an enzyme-linked immunosorbent assay, while nine different HIV-1 gp120s, two SIVcpz gp120s, and 128-to-139-substituted SIVmac239 gp120 did not bind jacalin. The ability or inability to bind jacalin thus correlated with the presence of the serine-threonine-rich stretch in the SIVmac and SIVsm gp120s and the absence of such stretches in the SIVcpz and HIV-1 gp120s. Consistent with sequence predictions, two HIV-2 gp120s bound jacalin, while one did not. These data demonstrate the presence of non- and monosialylated core 1 O-linked carbohydrate on the gp120s of SIVmac and SIVsm and the lack of these modifications on HIV-1 and SIVcpz gp120s."}

{"project":"SPECIES800","denotations":[{"id":"T1","span":{"begin":0,"end":29},"obj":"11723"},{"id":"T2","span":{"begin":39,"end":53},"obj":"9531"},{"id":"T3","span":{"begin":58,"end":72},"obj":"9544"},{"id":"T4","span":{"begin":334,"end":343},"obj":"11723"},{"id":"T5","span":{"begin":463,"end":472},"obj":"3489"},{"id":"T6","span":{"begin":596,"end":605},"obj":"11723"},{"id":"T7","span":{"begin":640,"end":649},"obj":"11723"},{"id":"T8","span":{"begin":753,"end":782},"obj":"11723"},{"id":"T9","span":{"begin":784,"end":787},"obj":"11723"},{"id":"T10","span":{"begin":908,"end":917},"obj":"11723"},{"id":"T11","span":{"begin":999,"end":1008},"obj":"11723"},{"id":"T12","span":{"begin":1158,"end":1161},"obj":"11723"},{"id":"T13","span":{"begin":1207,"end":1216},"obj":"11723"},{"id":"T14","span":{"begin":1237,"end":1255},"obj":"11676"},{"id":"T15","span":{"begin":1322,"end":1328},"obj":"11723"},{"id":"T16","span":{"begin":1333,"end":1338},"obj":"11723"},{"id":"T17","span":{"begin":1432,"end":1437},"obj":"11676"},{"id":"T18","span":{"begin":1450,"end":1456},"obj":"11723"},{"id":"T19","span":{"begin":1492,"end":1501},"obj":"11723"},{"id":"T20","span":{"begin":1649,"end":1655},"obj":"11723"},{"id":"T21","span":{"begin":1660,"end":1665},"obj":"11723"},{"id":"T22","span":{"begin":1714,"end":1720},"obj":"11723"},{"id":"T23","span":{"begin":1725,"end":1730},"obj":"11676"},{"id":"T24","span":{"begin":1781,"end":1786},"obj":"11709"},{"id":"T25","span":{"begin":1937,"end":1943},"obj":"11723"},{"id":"T26","span":{"begin":1948,"end":1953},"obj":"11723"},{"id":"T27","span":{"begin":1993,"end":1998},"obj":"11676"},{"id":"T28","span":{"begin":2003,"end":2009},"obj":"11723"}],"namespaces":[{"prefix":"_base","uri":"http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info\u0026id="}],"text":"Simian immunodeficiency virus from the sooty mangabey and rhesus macaque is modified with O-linked carbohydrate.\nAlthough stretches of serine and threonine are sometimes sites for O-linked carbohydrate attachment, specific sequence and structural determinants for O-linked attachment remain ill defined. The gp120 envelope protein of SIVmac239 contains a serine-threonine-rich stretch of amino acids at positions 128 to 139. Here we show that lectin protein from jackfruit seed (jacalin), which binds to non- and monosialylated core 1 O-linked carbohydrate, potently inhibited the replication of SIVmac239. Selection of a jacalin-resistant SIVmac239 variant population resulted in virus with specific substitutions within amino acids 128 to 139. Cloned simian immunodeficiency virus (SIV) variants with substitutions in the 128-to-139 region had infectivities equivalent to, or within 1 log unit of, that of SIVmac239 and were resistant to the inhibitory effects of jacalin. Characterization of the SIVmac239 gp120 O-linked glycome showed the presence of core 1 and core 2 O-linked carbohydrate; a 128-to-139-substituted variant gp120 from jacalin-resistant SIV lacked O-linked carbohydrate. Unlike that of SIVmac239, the replication of HIV-1 strain NL4-3 was resistant to inhibition by jacalin. Purified gp120s from four SIVmac and SIVsm strains bound jacalin strongly in an enzyme-linked immunosorbent assay, while nine different HIV-1 gp120s, two SIVcpz gp120s, and 128-to-139-substituted SIVmac239 gp120 did not bind jacalin. The ability or inability to bind jacalin thus correlated with the presence of the serine-threonine-rich stretch in the SIVmac and SIVsm gp120s and the absence of such stretches in the SIVcpz and HIV-1 gp120s. Consistent with sequence predictions, two HIV-2 gp120s bound jacalin, while one did not. These data demonstrate the presence of non- and monosialylated core 1 O-linked carbohydrate on the gp120s of SIVmac and SIVsm and the lack of these modifications on HIV-1 and SIVcpz gp120s."}