PubMed:20951743 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_20951743_1_0","span":{"begin":170,"end":194},"obj":"expanded"},{"id":"SS2_20951743_1_0","span":{"begin":196,"end":199},"obj":"abbr"},{"id":"SS1_20951743_1_1","span":{"begin":240,"end":268},"obj":"expanded"},{"id":"SS2_20951743_1_1","span":{"begin":270,"end":274},"obj":"abbr"}],"relations":[{"id":"AE1_20951743_1_0","pred":"abbreviatedTo","subj":"SS1_20951743_1_0","obj":"SS2_20951743_1_0"},{"id":"AE1_20951743_1_1","pred":"abbreviatedTo","subj":"SS1_20951743_1_1","obj":"SS2_20951743_1_1"}],"text":"Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1.\nA lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes O, A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples (n=396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples (n=95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%."}

{"project":"SPECIES800","denotations":[{"id":"T1","span":{"begin":122,"end":152},"obj":"12110"},{"id":"T2","span":{"begin":154,"end":155},"obj":"12110"},{"id":"T3","span":{"begin":160,"end":166},"obj":"12110"},{"id":"T4","span":{"begin":240,"end":268},"obj":"12110"},{"id":"T5","span":{"begin":270,"end":274},"obj":"12110"},{"id":"T6","span":{"begin":286,"end":287},"obj":"12110"},{"id":"T7","span":{"begin":289,"end":290},"obj":"12110"},{"id":"T8","span":{"begin":295,"end":301},"obj":"12110"},{"id":"T9","span":{"begin":328,"end":335},"obj":"9986"},{"id":"T10","span":{"begin":403,"end":411},"obj":"12110"},{"id":"T11","span":{"begin":413,"end":423},"obj":"12110"},{"id":"T12","span":{"begin":425,"end":438},"obj":"12110"},{"id":"T13","span":{"begin":453,"end":458},"obj":"10090"},{"id":"T14","span":{"begin":482,"end":491},"obj":"12110"},{"id":"T15","span":{"begin":493,"end":502},"obj":"12110"},{"id":"T16","span":{"begin":504,"end":518},"obj":"12110"},{"id":"T17","span":{"begin":625,"end":626},"obj":"12110"},{"id":"T18","span":{"begin":628,"end":629},"obj":"12110"},{"id":"T19","span":{"begin":634,"end":640},"obj":"12110"},{"id":"T20","span":{"begin":1079,"end":1083},"obj":"12110"},{"id":"T21","span":{"begin":1094,"end":1095},"obj":"12110"},{"id":"T22","span":{"begin":1097,"end":1098},"obj":"12110"},{"id":"T23","span":{"begin":1103,"end":1109},"obj":"12110"},{"id":"T24","span":{"begin":1230,"end":1246},"obj":"12110"}],"namespaces":[{"prefix":"_base","uri":"http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info\u0026id="}],"text":"Development and validation of a lateral flow immunoassay using colloidal gold for the identification of serotype-specific foot-and-mouth disease virus O, A and Asia 1.\nA lateral flow immunoassay (LFI) was developed to identify and diagnose foot-and-mouth disease virus (FMDV) serotypes O, A and Asia 1. Antibodies obtained from rabbits and guinea pigs immunized with cell-culture-adapted virus strains (O/CHA/99, A/GS/LX/66, Asia 1/CHN/05) and suckling-mouse adapted virus strains (O/AV99(L), A/AV88(L), Asia 1/YNBS/58) were used as capture antibodies. The diagnostic kit included three immunochromatographic strips of types O, A and Asia 1, and the type-specific results were confirmed by color on the test lines of the three strips. The LFI was evaluated using epithelial and vesicular samples (n=396) prepared from current and historical field samples (provide by the National Foot-and-Mouth Disease Reference Laboratory of China at Lanzhou Veterinary Research Institute). Negative samples (n=95) were collected from healthy animals. The diagnostic sensitivity of the LFI for FMDV serotypes O, A and Asia 1 was 88.3% compared to 89.7% obtained by the reference method of indirect-sandwich ELISA. The sensitivity of the LFI for FMDV type Asia 1 was higher at 92.1% compared to 90.5% for the ELISA. The specificity of the LFI was 97.1% compared with 97.4%."}