| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-144 |
Sentence |
denotes |
Differential gene expression in benign prostate epithelium of men with and without prostate cancer: evidence for a prostate cancer field effect. |
| TextSentencer_T2 |
145-156 |
Sentence |
denotes |
BACKGROUND: |
| TextSentencer_T3 |
157-328 |
Sentence |
denotes |
Several malignancies are known to exhibit a "field effect," whereby regions beyond tumor boundaries harbor histologic or molecular changes that are associated with cancer. |
| TextSentencer_T4 |
329-499 |
Sentence |
denotes |
We sought to determine if histologically benign prostate epithelium collected from men with prostate cancer exhibits features indicative of premalignancy or field effect. |
| TextSentencer_T5 |
500-520 |
Sentence |
denotes |
EXPERIMENTAL DESIGN: |
| TextSentencer_T6 |
521-700 |
Sentence |
denotes |
Prostate needle biopsies from 15 men with high-grade (Gleason 8-10) prostate cancer and 15 age- and body mass index-matched controls were identified from a biospecimen repository. |
| TextSentencer_T7 |
701-783 |
Sentence |
denotes |
Benign epithelia from each patient were isolated by laser capture microdissection. |
| TextSentencer_T8 |
784-851 |
Sentence |
denotes |
RNA was isolated, amplified, and used for microarray hybridization. |
| TextSentencer_T9 |
852-936 |
Sentence |
denotes |
Quantitative PCR was used to determine the expression of specific genes of interest. |
| TextSentencer_T10 |
937-1014 |
Sentence |
denotes |
Alterations in protein expression were analyzed through immunohistochemistry. |
| TextSentencer_T11 |
1015-1023 |
Sentence |
denotes |
RESULTS: |
| TextSentencer_T12 |
1024-1190 |
Sentence |
denotes |
Overall patterns of gene expression in microdissected benign prostate-associated benign epithelium (BABE) and cancer-associated benign epithelium (CABE) were similar. |
| TextSentencer_T13 |
1191-1337 |
Sentence |
denotes |
Two genes previously associated with prostate cancer, PSMA and SSTR1, were significantly upregulated in the CABE group (false discovery rate <1%). |
| TextSentencer_T14 |
1338-1611 |
Sentence |
denotes |
Expression of other prostate cancer-associated genes, including ERG, HOXC4, HOXC5, and MME, were also increased in CABE by quantitative reverse transcription-PCR, although other genes commonly altered in prostate cancer were not different between the BABE and CABE samples. |
| TextSentencer_T15 |
1612-1714 |
Sentence |
denotes |
The expression of MME and PSMA proteins on immunohistochemistry coincided with their mRNA alterations. |
| TextSentencer_T16 |
1715-1726 |
Sentence |
denotes |
CONCLUSION: |
| TextSentencer_T17 |
1727-1839 |
Sentence |
denotes |
Gene expression profiles between benign epithelia of patients with and without prostate cancer are very similar. |
| TextSentencer_T18 |
1840-1995 |
Sentence |
denotes |
However, these tissues exhibit differences in the expression levels of several genes previously associated with prostate cancer development or progression. |
| TextSentencer_T19 |
1996-2087 |
Sentence |
denotes |
These differences may comprise a field effect and represent early events in carcinogenesis. |
| T1 |
0-144 |
Sentence |
denotes |
Differential gene expression in benign prostate epithelium of men with and without prostate cancer: evidence for a prostate cancer field effect. |
| T2 |
145-156 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
157-328 |
Sentence |
denotes |
Several malignancies are known to exhibit a "field effect," whereby regions beyond tumor boundaries harbor histologic or molecular changes that are associated with cancer. |
| T4 |
329-499 |
Sentence |
denotes |
We sought to determine if histologically benign prostate epithelium collected from men with prostate cancer exhibits features indicative of premalignancy or field effect. |
| T5 |
500-520 |
Sentence |
denotes |
EXPERIMENTAL DESIGN: |
| T6 |
521-700 |
Sentence |
denotes |
Prostate needle biopsies from 15 men with high-grade (Gleason 8-10) prostate cancer and 15 age- and body mass index-matched controls were identified from a biospecimen repository. |
| T7 |
701-783 |
Sentence |
denotes |
Benign epithelia from each patient were isolated by laser capture microdissection. |
| T8 |
784-851 |
Sentence |
denotes |
RNA was isolated, amplified, and used for microarray hybridization. |
| T9 |
852-936 |
Sentence |
denotes |
Quantitative PCR was used to determine the expression of specific genes of interest. |
| T10 |
937-1014 |
Sentence |
denotes |
Alterations in protein expression were analyzed through immunohistochemistry. |
| T11 |
1015-1023 |
Sentence |
denotes |
RESULTS: |
| T12 |
1024-1190 |
Sentence |
denotes |
Overall patterns of gene expression in microdissected benign prostate-associated benign epithelium (BABE) and cancer-associated benign epithelium (CABE) were similar. |
| T13 |
1191-1337 |
Sentence |
denotes |
Two genes previously associated with prostate cancer, PSMA and SSTR1, were significantly upregulated in the CABE group (false discovery rate <1%). |
| T14 |
1338-1611 |
Sentence |
denotes |
Expression of other prostate cancer-associated genes, including ERG, HOXC4, HOXC5, and MME, were also increased in CABE by quantitative reverse transcription-PCR, although other genes commonly altered in prostate cancer were not different between the BABE and CABE samples. |
| T15 |
1612-1714 |
Sentence |
denotes |
The expression of MME and PSMA proteins on immunohistochemistry coincided with their mRNA alterations. |
| T16 |
1715-1726 |
Sentence |
denotes |
CONCLUSION: |
| T17 |
1727-1839 |
Sentence |
denotes |
Gene expression profiles between benign epithelia of patients with and without prostate cancer are very similar. |
| T18 |
1840-1995 |
Sentence |
denotes |
However, these tissues exhibit differences in the expression levels of several genes previously associated with prostate cancer development or progression. |
| T19 |
1996-2087 |
Sentence |
denotes |
These differences may comprise a field effect and represent early events in carcinogenesis. |