PubMed:20847188
Annnotations
{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/20847188","sourcedb":"PubMed","sourceid":"20847188","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/20847188","text":"Relaxed acceptor site specificity of bacterial oligosaccharyltransferase in vivo.\nA number of proteobacteria carry the genetic information to perform N-linked glycosylation, but only the protein glycosylation (pgl) pathway of Campylobacter jejuni has been studied to date. Here, we report that the pgl gene cluster of Campylobacter lari encodes for a functional glycosylation machinery that can be reconstituted in Escherichia coli. We determined that the N-glycan produced in this system consisted of a linear hexasaccharide. We found that the oligosaccharyltransferase (OST) of C. lari conserved a predominant specificity for the primary sequence D/E-X(-1)-N-X(+1)-S/T (where X(-1) and X(+1) can be any amino acid but proline). At the same time, we observed that this enzyme exhibited a relaxed specificity toward the acceptor site and modified asparagine residues of a protein at sequences DANSG and NNNST. Moreover, C. lari pgl glycosylated a native E. coli protein. Bacterial N-glycosylation appears as a useful tool to establish a molecular description of how single-subunit OSTs perform selection of glycosyl acceptor sites.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":81},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":82,"end":272},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":273,"end":432},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":433,"end":526},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":527,"end":729},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":730,"end":909},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":910,"end":970},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":971,"end":1131},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":81},"obj":"Sentence"},{"id":"T2","span":{"begin":82,"end":272},"obj":"Sentence"},{"id":"T3","span":{"begin":273,"end":432},"obj":"Sentence"},{"id":"T4","span":{"begin":433,"end":526},"obj":"Sentence"},{"id":"T5","span":{"begin":527,"end":729},"obj":"Sentence"},{"id":"T6","span":{"begin":730,"end":909},"obj":"Sentence"},{"id":"T7","span":{"begin":910,"end":970},"obj":"Sentence"},{"id":"T8","span":{"begin":971,"end":1131},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":81},"obj":"Sentence"},{"id":"T2","span":{"begin":82,"end":272},"obj":"Sentence"},{"id":"T3","span":{"begin":273,"end":432},"obj":"Sentence"},{"id":"T4","span":{"begin":433,"end":526},"obj":"Sentence"},{"id":"T5","span":{"begin":527,"end":729},"obj":"Sentence"},{"id":"T6","span":{"begin":730,"end":909},"obj":"Sentence"},{"id":"T7","span":{"begin":910,"end":970},"obj":"Sentence"},{"id":"T8","span":{"begin":971,"end":1131},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"}]},{"project":"GlycoBiology-PACDB","denotations":[{"id":"_T1","span":{"begin":226,"end":246},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC257,LEC564"},{"id":"_T2","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417"},{"id":"_T3","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407"},{"id":"_T4","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754"},{"id":"_T5","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640"},{"id":"_T6","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620"},{"id":"_T7","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487"},{"id":"_T8","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354"},{"id":"_T9","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452"},{"id":"_T10","span":{"begin":415,"end":431},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-PACDB"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-PACDB"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":187,"end":194},"obj":"FMAID:67257"},{"id":"_T2","span":{"begin":187,"end":194},"obj":"FMAID:165447"},{"id":"_T3","span":{"begin":302,"end":306},"obj":"FMAID:198663"},{"id":"_T4","span":{"begin":302,"end":314},"obj":"FMAID:198017"},{"id":"_T5","span":{"begin":302,"end":314},"obj":"FMAID:84082"},{"id":"_T6","span":{"begin":705,"end":715},"obj":"FMAID:82739"},{"id":"_T7","span":{"begin":705,"end":715},"obj":"FMAID:196728"},{"id":"_T8","span":{"begin":720,"end":727},"obj":"FMAID:196750"},{"id":"_T9","span":{"begin":720,"end":727},"obj":"FMAID:82761"},{"id":"_T10","span":{"begin":847,"end":857},"obj":"FMAID:196739"},{"id":"_T11","span":{"begin":847,"end":857},"obj":"FMAID:82750"},{"id":"_T12","span":{"begin":872,"end":879},"obj":"FMAID:165447"},{"id":"_T13","span":{"begin":872,"end":879},"obj":"FMAID:67257"},{"id":"_T14","span":{"begin":962,"end":969},"obj":"FMAID:67257"},{"id":"_T15","span":{"begin":962,"end":969},"obj":"FMAID:165447"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T15","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":210,"end":213},"obj":"http://www.uniprot.org/uniprot/Q9CQ60"},{"id":"T2","span":{"begin":298,"end":301},"obj":"http://www.uniprot.org/uniprot/Q9CQ60"},{"id":"T3","span":{"begin":928,"end":931},"obj":"http://www.uniprot.org/uniprot/Q9CQ60"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"},{"subj":"T2","pred":"source","obj":"uniprot-mouse"},{"subj":"T3","pred":"source","obj":"uniprot-mouse"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":94,"end":108},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/28216"},{"id":"T2","span":{"begin":94,"end":108},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/580370"},{"id":"T3","span":{"begin":94,"end":108},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/380367"},{"id":"T4","span":{"begin":94,"end":108},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/1224"},{"id":"T5","span":{"begin":226,"end":246},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/197"},{"id":"T6","span":{"begin":415,"end":426},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/561"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T4","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T5","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T6","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":159,"end":172},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T2","span":{"begin":195,"end":208},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T3","span":{"begin":362,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T4","span":{"begin":932,"end":944},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T5","span":{"begin":1107,"end":1115},"obj":"http://purl.obolibrary.org/obo/GO_0070085"},{"id":"T6","span":{"begin":187,"end":208},"obj":"http://purl.obolibrary.org/obo/GO_0006486"},{"id":"T7","span":{"begin":210,"end":213},"obj":"http://purl.obolibrary.org/obo/GO_0004598"},{"id":"T8","span":{"begin":298,"end":301},"obj":"http://purl.obolibrary.org/obo/GO_0004598"},{"id":"T9","span":{"begin":928,"end":931},"obj":"http://purl.obolibrary.org/obo/GO_0004598"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"},{"subj":"T9","pred":"source","obj":"GO-BP"}]},{"project":"Allie","denotations":[{"id":"SS1_20847188_1_0","span":{"begin":187,"end":208},"obj":"expanded"},{"id":"SS2_20847188_1_0","span":{"begin":210,"end":213},"obj":"abbr"},{"id":"SS1_20847188_4_0","span":{"begin":545,"end":570},"obj":"expanded"},{"id":"SS2_20847188_4_0","span":{"begin":572,"end":575},"obj":"abbr"}],"relations":[{"id":"AE1_20847188_1_0","pred":"abbreviatedTo","subj":"SS1_20847188_1_0","obj":"SS2_20847188_1_0"},{"id":"AE1_20847188_4_0","pred":"abbreviatedTo","subj":"SS1_20847188_4_0","obj":"SS2_20847188_4_0"}],"attributes":[{"subj":"SS1_20847188_1_0","pred":"source","obj":"Allie"},{"subj":"SS2_20847188_1_0","pred":"source","obj":"Allie"},{"subj":"SS1_20847188_4_0","pred":"source","obj":"Allie"},{"subj":"SS2_20847188_4_0","pred":"source","obj":"Allie"}]},{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":456,"end":464},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-Motifs"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":94,"end":108},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":226,"end":246},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":318,"end":336},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":415,"end":431},"obj":"OrganismTaxon"},{"id":"T5","span":{"begin":954,"end":961},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"1224"},{"id":"A2","pred":"db_id","subj":"T2","obj":"197"},{"id":"A3","pred":"db_id","subj":"T3","obj":"201"},{"id":"A4","pred":"db_id","subj":"T4","obj":"562"},{"id":"A5","pred":"db_id","subj":"T5","obj":"562"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"},{"subj":"T4","pred":"source","obj":"NCBITAXON"},{"subj":"T5","pred":"source","obj":"NCBITAXON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ec93b0","default":true},{"id":"GlycoBiology-PACDB","color":"#93caec"},{"id":"GlycoBiology-FMA","color":"#e4ec93"},{"id":"uniprot-mouse","color":"#da93ec"},{"id":"GlycoBiology-NCBITAXON","color":"#93ecc0"},{"id":"GO-BP","color":"#eca693"},{"id":"Allie","color":"#939aec"},{"id":"GlycoBiology-Motifs","color":"#b4ec93"},{"id":"NCBITAXON","color":"#ec93ce"}]}]}}