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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 163-618 BACKGROUND denotes The canonical Wnt signaling is concurrently important for osteoblast differentiation and myeloma cell proliferation. Its activation in myeloma cells and its inhibition in osteoblasts and their progenitors have been identified in the previous studies. Osteoblast progenitors and myeloma cells from a myeloma patient share the same bone marrow (BM) microenvironment, but respond differently to DKK-1 secreted by myeloma cells. The mechanisms remain unclear.
T2 628-1255 METHODS denotes Primary multiple myeloma (MM) cells were isolated from BM mononuclear cells of 12 MM patients. Human bone marrow stromal cells (SCs) were obtained from BM adherent cells of these MM patients and 10 healthy donors. The mRNA expression levels of DKK-1 binding receptor LRP5/6 and Kremen1/2 (Krm1/2) were analyzed by Real-time PCR in human myeloma cell line (HMCL) RPMI-8226, NCI-H929, U266, LP-1, CZ-1, KM-3, Sko-007, primary myeloma cells and SCs from 12 MM patients and SCs from 10 healthy donors. The binding capability of DKK-1 binding receptors to DKK-1 on primary myeloma cells and SCs was detected by flow cytometry assay.
T3 1265-1893 RESULTS denotes The mRNA expression levels of DKK-1 binding receptor LRP5/6 and Krm1/2 in SCs from patients with MM were significantly higher than those in myeloma cells and in SCs from healthy donors. The binding capability to DKK-1of DKK-1 binding receptors on SCs from MM patients was obviously higher than those on myeloma cells and SCs from healthy donors by flow cytometry assay. Similar to the effects of coculture with rhDKK1, coculture of SCs from healthy donors with myeloma cells in the presence or absence of a Transwell insert did up-regulate SCs' mRNA levels of LRP5/6 and Krm1/2, and down-regulate their mRNA levels of β-catenin.
T4 1906-2211 CONCLUSIONS denotes Compared with myeloma cells, the SCs from MM patients overexpress DKK-1 binding receptors LRP5/6 and Krm1/2 in response to DKK-1 secreted by myeloma cells, which results in intracellular Wnt signaling inhibition. Our study provides a novel insight into mechanisms of myeloma associated osteolytic lesions.

DisGeNET

Id Subject Object Predicate Lexical cue
T0 133-138 gene:22943 denotes DKK-1
T1 142-149 disease:C0026764 denotes myeloma
T2 133-138 gene:22943 denotes DKK-1
T3 72-79 disease:C0026764 denotes myeloma
T4 27-32 gene:22943 denotes DKK-1
T5 72-79 disease:C0026764 denotes myeloma
T6 27-32 gene:22943 denotes DKK-1
T7 142-149 disease:C0026764 denotes myeloma
T8 555-560 gene:22943 denotes DKK-1
T9 441-448 disease:C0026764 denotes myeloma
T10 555-560 gene:22943 denotes DKK-1
T11 462-469 disease:C0026764 denotes myeloma
T12 1329-1333 gene:83999 denotes Krm1
T13 1362-1364 disease:C0026764 denotes MM
T14 1295-1300 gene:22943 denotes DKK-1
T15 1405-1412 disease:C0026764 denotes myeloma
T16 1295-1300 gene:22943 denotes DKK-1
T17 1362-1364 disease:C0026764 denotes MM
R1 T0 T1 associated_with DKK-1,myeloma
R2 T2 T3 associated_with DKK-1,myeloma
R3 T4 T5 associated_with DKK-1,myeloma
R4 T6 T7 associated_with DKK-1,myeloma
R5 T8 T9 associated_with DKK-1,myeloma
R6 T10 T11 associated_with DKK-1,myeloma
R7 T12 T13 associated_with Krm1,MM
R8 T14 T15 associated_with DKK-1,myeloma
R9 T16 T17 associated_with DKK-1,MM

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 221-231 HP_0011846 denotes osteoblast
T2 334-345 HP_0011846 denotes osteoblasts
T3 414-424 HP_0011846 denotes Osteoblast

Allie

Id Subject Object Predicate Lexical cue
SS1_20846389_4_0 493-504 expanded denotes bone marrow
SS2_20846389_4_0 506-508 abbr denotes BM
SS1_20846389_7_0 636-652 expanded denotes multiple myeloma
SS2_20846389_7_0 654-656 abbr denotes MM
SS1_20846389_8_0 741-754 expanded denotes stromal cells
SS2_20846389_8_0 756-759 abbr denotes SCs
SS1_20846389_9_0 959-982 expanded denotes human myeloma cell line
SS2_20846389_9_0 984-988 abbr denotes HMCL
AE1_20846389_4_0 SS1_20846389_4_0 SS2_20846389_4_0 abbreviatedTo bone marrow,BM
AE1_20846389_7_0 SS1_20846389_7_0 SS2_20846389_7_0 abbreviatedTo multiple myeloma,MM
AE1_20846389_8_0 SS1_20846389_8_0 SS2_20846389_8_0 abbreviatedTo stromal cells,SCs
AE1_20846389_9_0 SS1_20846389_9_0 SS2_20846389_9_0 abbreviatedTo human myeloma cell line,HMCL

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
20846389-0#27#32#gene22943 27-32 gene22943 denotes DKK-1
20846389-0#133#138#gene22943 133-138 gene22943 denotes DKK-1
20846389-0#72#79#diseaseC0026764 72-79 diseaseC0026764 denotes myeloma
20846389-0#142#149#diseaseC0026764 142-149 diseaseC0026764 denotes myeloma
20846389-0#72#79#diseaseC0026764 72-79 diseaseC0026764 denotes myeloma
20846389-0#142#149#diseaseC0026764 142-149 diseaseC0026764 denotes myeloma
20846389-10#190#194#gene4041 1825-1829 gene4041 denotes LRP5
20846389-10#91#98#diseaseC0026764 1726-1733 diseaseC0026764 denotes myeloma
20846389-8#64#68#gene83999 1329-1333 gene83999 denotes Krm1
20846389-8#97#99#diseaseC0026764 1362-1364 diseaseC0026764 denotes MM
27#32#gene2294372#79#diseaseC0026764 20846389-0#27#32#gene22943 20846389-0#72#79#diseaseC0026764 associated_with DKK-1,myeloma
27#32#gene22943142#149#diseaseC0026764 20846389-0#27#32#gene22943 20846389-0#142#149#diseaseC0026764 associated_with DKK-1,myeloma
27#32#gene2294372#79#diseaseC0026764 20846389-0#27#32#gene22943 20846389-0#72#79#diseaseC0026764 associated_with DKK-1,myeloma
27#32#gene22943142#149#diseaseC0026764 20846389-0#27#32#gene22943 20846389-0#142#149#diseaseC0026764 associated_with DKK-1,myeloma
133#138#gene2294372#79#diseaseC0026764 20846389-0#133#138#gene22943 20846389-0#72#79#diseaseC0026764 associated_with DKK-1,myeloma
133#138#gene22943142#149#diseaseC0026764 20846389-0#133#138#gene22943 20846389-0#142#149#diseaseC0026764 associated_with DKK-1,myeloma
133#138#gene2294372#79#diseaseC0026764 20846389-0#133#138#gene22943 20846389-0#72#79#diseaseC0026764 associated_with DKK-1,myeloma
133#138#gene22943142#149#diseaseC0026764 20846389-0#133#138#gene22943 20846389-0#142#149#diseaseC0026764 associated_with DKK-1,myeloma
190#194#gene404191#98#diseaseC0026764 20846389-10#190#194#gene4041 20846389-10#91#98#diseaseC0026764 associated_with LRP5,myeloma
64#68#gene8399997#99#diseaseC0026764 20846389-8#64#68#gene83999 20846389-8#97#99#diseaseC0026764 associated_with Krm1,MM