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PubMed:20798114 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 548-554 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T2 675-681 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 548-554 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO
T2 675-681 Glycan_Motif denotes fucose https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 548-554 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose
T2 675-681 https://glytoucan.org/Structures/Glycans/G82576YO denotes fucose

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
TextSentencer_T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
TextSentencer_T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
TextSentencer_T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
TextSentencer_T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
TextSentencer_T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
TextSentencer_T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
TextSentencer_T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
TextSentencer_T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
TextSentencer_T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
TextSentencer_T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
TextSentencer_T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
TextSentencer_T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
TextSentencer_T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
TextSentencer_T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.
T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.
T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T2 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T3 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T4 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T7 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T8 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T9 1041-1057 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 95-110 FMAID:62931 denotes lectin. Lectins
_T2 95-110 FMAID:165243 denotes lectin. Lectins
_T3 95-110 FMAID:61795 denotes lectin. Lectins
_T4 95-110 FMAID:167265 denotes lectin. Lectins
_T5 181-194 FMAID:82737 denotes carbohydrates
_T6 181-194 FMAID:197276 denotes carbohydrates
_T7 214-227 FMAID:82737 denotes carbohydrates
_T8 214-227 FMAID:197276 denotes carbohydrates
_T9 424-437 FMAID:62925 denotes glycoproteins
_T10 424-437 FMAID:167256 denotes glycoproteins
_T11 548-554 FMAID:196784 denotes fucose
_T12 548-554 FMAID:82790 denotes fucose
_T13 566-582 FMAID:196731 denotes oligosaccharides
_T14 566-582 FMAID:82742 denotes oligosaccharides
_T15 675-681 FMAID:82790 denotes fucose
_T16 675-681 FMAID:196784 denotes fucose
_T17 969-984 FMAID:198074 denotes coding sequence
_T18 1022-1037 FMAID:166112 denotes tagged proteins
_T19 1029-1037 FMAID:67257 denotes proteins
_T20 1029-1037 FMAID:165447 denotes proteins
_T21 1160-1167 FMAID:50594 denotes surface
_T22 1160-1167 FMAID:146300 denotes surface
_T23 1316-1318 FMAID:63523 denotes S2
_T24 1316-1318 FMAID:167751 denotes S2
_T25 1374-1390 FMAID:82742 denotes oligosaccharides
_T26 1374-1390 FMAID:196731 denotes oligosaccharides
_T27 1546-1562 FMAID:196731 denotes oligosaccharides
_T28 1546-1562 FMAID:82742 denotes oligosaccharides
_T29 1612-1614 FMAID:63523 denotes S2
_T30 1612-1614 FMAID:167751 denotes S2
_T31 1666-1682 FMAID:82742 denotes oligosaccharides
_T32 1666-1682 FMAID:196731 denotes oligosaccharides
_T33 1718-1720 FMAID:167751 denotes S2
_T34 1718-1720 FMAID:63523 denotes S2
_T35 1757-1773 FMAID:82742 denotes oligosaccharides
_T36 1757-1773 FMAID:196731 denotes oligosaccharides
_T37 2060-2072 FMAID:197276 denotes carbohydrate
_T38 2060-2072 FMAID:82737 denotes carbohydrate

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 78-85 http://purl.bioontology.org/ontology/NCBITAXON/5187 denotes Aleuria
T2 78-94 http://purl.bioontology.org/ontology/NCBITAXON/5188 denotes Aleuria aurantia
T3 522-529 http://purl.bioontology.org/ontology/NCBITAXON/5187 denotes Aleuria
T4 522-538 http://purl.bioontology.org/ontology/NCBITAXON/5188 denotes Aleuria aurantia
T5 956-961 http://purl.bioontology.org/ontology/NCBITAXON/79338 denotes codon
T6 956-961 http://purl.bioontology.org/ontology/NCBITAXON/722432 denotes codon
T7 1041-1052 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia

GO-BP

Id Subject Object Predicate Lexical cue
T1 383-396 http://purl.obolibrary.org/obo/GO_0000752 denotes agglutination
T2 383-396 http://purl.obolibrary.org/obo/GO_0007157 denotes agglutination
T3 814-824 http://purl.obolibrary.org/obo/GO_0015297 denotes exchanging
T4 1362-1373 http://purl.obolibrary.org/obo/GO_0036065 denotes fucosylated
T5 1534-1545 http://purl.obolibrary.org/obo/GO_0036065 denotes fucosylated
T6 1654-1665 http://purl.obolibrary.org/obo/GO_0036065 denotes fucosylated
T7 1741-1751 http://purl.obolibrary.org/obo/GO_0097503 denotes sialylated
T8 2003-2013 http://purl.obolibrary.org/obo/GO_0032502 denotes developing

GO-MF

Id Subject Object Predicate Lexical cue
T1 209-213 http://purl.obolibrary.org/obo/GO_0070026 denotes bind
T2 1357-1361 http://purl.obolibrary.org/obo/GO_0070026 denotes bind
T3 539-544 http://purl.obolibrary.org/obo/GO_0070026 denotes binds
T4 657-664 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T5 1512-1519 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T6 1099-1106 http://purl.obolibrary.org/obo/GO_0070026 denotes Binding
T7 209-213 http://purl.obolibrary.org/obo/GO_0003680 denotes bind
T8 1357-1361 http://purl.obolibrary.org/obo/GO_0003680 denotes bind
T9 539-544 http://purl.obolibrary.org/obo/GO_0003680 denotes binds
T10 657-664 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T11 1512-1519 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T12 1099-1106 http://purl.obolibrary.org/obo/GO_0003680 denotes Binding
T13 209-213 http://purl.obolibrary.org/obo/GO_0017091 denotes bind
T14 1357-1361 http://purl.obolibrary.org/obo/GO_0017091 denotes bind
T15 539-544 http://purl.obolibrary.org/obo/GO_0017091 denotes binds
T16 657-664 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T17 1512-1519 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T18 1099-1106 http://purl.obolibrary.org/obo/GO_0017091 denotes Binding
T19 209-213 http://purl.obolibrary.org/obo/GO_0005488 denotes bind
T20 1357-1361 http://purl.obolibrary.org/obo/GO_0005488 denotes bind
T21 539-544 http://purl.obolibrary.org/obo/GO_0005488 denotes binds
T22 657-664 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T23 1512-1519 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T24 1099-1106 http://purl.obolibrary.org/obo/GO_0005488 denotes Binding
T25 209-227 http://purl.obolibrary.org/obo/GO_0030246 denotes bind carbohydrates
T26 539-554 http://purl.obolibrary.org/obo/GO_0042806 denotes binds to fucose
T27 1022-1037 http://purl.obolibrary.org/obo/GO_0031386 denotes tagged proteins

Allie

Id Subject Object Predicate Lexical cue
SS1_20798114_10_0 1270-1283 expanded denotes monomeric AAL
SS2_20798114_10_0 1285-1289 abbr denotes mAAL
SS1_20798114_11_0 1468-1483 expanded denotes recombinant AAL
SS2_20798114_11_0 1485-1489 abbr denotes rAAL
AE1_20798114_10_0 SS1_20798114_10_0 SS2_20798114_10_0 abbreviatedTo monomeric AAL,mAAL
AE1_20798114_11_0 SS1_20798114_11_0 SS2_20798114_11_0 abbreviatedTo recombinant AAL,rAAL

Lectin

Id Subject Object Predicate Lexical cue
Lectin_T1 1485-1489 https://acgg.asia/db/lfdb/LfDB0396 denotes rAAL
Lectin_T2 1566-1570 https://acgg.asia/db/lfdb/LfDB0396 denotes rAAL
Lectin_T3 1703-1707 https://acgg.asia/db/lfdb/LfDB0396 denotes rAAL

Lectin-Jamboree-small

Id Subject Object Predicate Lexical cue glycosmos_id
T1 723-726 Lectin denotes AAL GL_001404
T2 794-797 Lectin denotes AAL GL_001404
T3 871-874 Lectin denotes AAL GL_001404
T4 990-993 Lectin denotes AAL GL_001404
T5 1129-1132 Lectin denotes AAL GL_001404
T6 1280-1283 Lectin denotes AAL GL_001404
T7 1311-1314 Lectin denotes AAL GL_001404
T8 1319-1322 Lectin denotes AAL GL_001404
T9 1480-1483 Lectin denotes AAL GL_001404
T10 1615-1618 Lectin denotes AAL GL_001404
T11 1721-1724 Lectin denotes AAL GL_001404

Lectin-Jamboree-Sentence

Id Subject Object Predicate Lexical cue
T1 0-102 Sentence denotes Production and characterization of a monomeric form and a single-site form of Aleuria aurantia lectin.
T2 103-195 Sentence denotes Lectins have widely been used in structural and functional studies of complex carbohydrates.
T3 196-298 Sentence denotes They usually bind carbohydrates with relatively low affinity, but compensate for this by multivalency.
T4 299-501 Sentence denotes This multivalent nature of lectins can sometimes produce unwanted reactions such as agglutination or precipitation of target glycoproteins, when using them in different biological and analytical assays.
T5 502-583 Sentence denotes The mushroom lectin Aleuria aurantia binds to fucose-containing oligosaccharides.
T6 584-682 Sentence denotes It is composed of two identical subunits, and each subunit contains five binding sites for fucose.
T7 683-773 Sentence denotes In this study, two forms of recombinant AAL were produced using site-directed mutagenesis.
T8 774-985 Sentence denotes A monomeric form of AAL was produced by exchanging Tyr6 with Arg6, and a single-site fragment of AAL was produced by insertion of an NdeI restriction enzyme cleavage site and a stop codon in the coding sequence.
T9 986-1098 Sentence denotes The AAL forms were expressed as His-tagged proteins in Escherichia coli and purified by affinity chromatography.
T10 1099-1260 Sentence denotes Binding properties of the two AAL forms were performed using surface plasmon resonance, enzyme-linked lectin assay analyses and isothermal titration calorimetry.
T11 1261-1391 Sentence denotes Both the monomeric AAL (mAAL) and the single-site AAL (S2-AAL) forms retained their capacity to bind fucosylated oligosaccharides.
T12 1392-1611 Sentence denotes However, both constructs exhibited properties that differed from the intact recombinant AAL (rAAL). mAAL showed similar binding affinities to fucosylated oligosaccharides as rAAL, but had less hemagglutinating capacity.
T13 1612-1774 Sentence denotes S2-AAL showed a lower binding affinity to fucosylated oligosaccharides and, in contrast to rAAL and mAAL, S2-AAL did not bind to sialylated fuco-oligosaccharides.
T14 1775-1960 Sentence denotes The study shows that molecular engineering is a highly useful tool for producing lectins with more defined properties such as decreased valency and defined specificities and affinities.
T15 1961-2082 Sentence denotes Thus, this approach has high potential in developing reliable diagnostic and biological assays for carbohydrate analysis.

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 78-94 OrganismTaxon denotes Aleuria aurantia 5188
T2 522-538 OrganismTaxon denotes Aleuria aurantia 5188
T3 1041-1057 OrganismTaxon denotes Escherichia coli 562