PubMed:20308281 JSONTXT

{"project":"Allie","denotations":[{"id":"SS1_20308281_2_0","span":{"begin":285,"end":318},"obj":"expanded"},{"id":"SS2_20308281_2_0","span":{"begin":320,"end":324},"obj":"abbr"},{"id":"SS1_20308281_2_1","span":{"begin":378,"end":390},"obj":"expanded"},{"id":"SS2_20308281_2_1","span":{"begin":392,"end":394},"obj":"abbr"},{"id":"SS1_20308281_4_0","span":{"begin":669,"end":692},"obj":"expanded"},{"id":"SS2_20308281_4_0","span":{"begin":694,"end":697},"obj":"abbr"}],"relations":[{"id":"AE1_20308281_2_0","pred":"abbreviatedTo","subj":"SS1_20308281_2_0","obj":"SS2_20308281_2_0"},{"id":"AE1_20308281_2_1","pred":"abbreviatedTo","subj":"SS1_20308281_2_1","obj":"SS2_20308281_2_1"},{"id":"AE1_20308281_4_0","pred":"abbreviatedTo","subj":"SS1_20308281_4_0","obj":"SS2_20308281_4_0"}],"text":"Caenorhabditis elegans fibroblast growth factor receptor signaling can occur independently of the multi-substrate adaptor FRS2.\nThe components of receptor tyrosine kinase signaling complexes help to define the specificity of the effects of their activation. The Caenorhabditis elegans fibroblast growth factor receptor (FGFR), EGL-15, regulates a number of processes, including sex myoblast (SM) migration guidance and fluid homeostasis, both of which require a Grb2/Sos/Ras cassette of signaling components. Here we show that SEM-5/Grb2 can bind directly to EGL-15 to mediate SM chemoattraction. A yeast two-hybrid screen identified SEM-5 as able to interact with the carboxy-terminal domain (CTD) of EGL-15, a domain that is specifically required for SM chemoattraction. This interaction requires the SEM-5 SH2-binding motifs present in the CTD (Y(1009) and Y(1087)), and these sites are required for the CTD role of EGL-15 in SM chemoattraction. SEM-5, but not the SEM-5 binding sites located in the CTD, is required for the fluid homeostasis function of EGL-15, indicating that SEM-5 can link to EGL-15 through an alternative mechanism. The multi-substrate adaptor protein FRS2 serves to link vertebrate FGFRs to Grb2. In C. elegans, an FRS2-like gene, rog-1, functions upstream of a Ras/MAPK pathway for oocyte maturation but is not required for EGL-15 function. Thus, unlike the vertebrate FGFRs, which require the multi-substrate adaptor FRS2 to recruit Grb2, EGL-15 can recruit SEM-5/Grb2 directly."}