| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-61 |
Sentence |
denotes |
Monomeric red fluorescent proteins with a large Stokes shift. |
| TextSentencer_T2 |
62-158 |
Sentence |
denotes |
Two-photon microscopy has advanced fluorescence imaging of cellular processes in living animals. |
| TextSentencer_T3 |
159-374 |
Sentence |
denotes |
Fluorescent proteins in the blue-green wavelength range are widely used in two-photon microscopy; however, the use of red fluorescent proteins is limited by the low power output of Ti-Sapphire lasers above 1,000 nm. |
| TextSentencer_T4 |
375-584 |
Sentence |
denotes |
To overcome this limitation we have developed two red fluorescent proteins, LSS-mKate1 and LSS-mKate2, which possess large Stokes shifts with excitation/emission maxima at 463/624 and 460/605 nm, respectively. |
| TextSentencer_T5 |
585-711 |
Sentence |
denotes |
These LSS-mKates are characterized by high pH stability, photostability, rapid chromophore maturation, and monomeric behavior. |
| TextSentencer_T6 |
712-834 |
Sentence |
denotes |
They lack absorbance in the green region, providing an additional red color to the commonly used red fluorescent proteins. |
| TextSentencer_T7 |
835-991 |
Sentence |
denotes |
Substantial overlap between the two-photon excitation spectra of the LSS-mKates and blue-green fluorophores enables multicolor imaging using a single laser. |
| TextSentencer_T8 |
992-1190 |
Sentence |
denotes |
We applied this approach to a mouse xenograft model of breast cancer to intravitally study the motility and Golgi-nucleus alignment of tumor cells as a function of their distance from blood vessels. |
| TextSentencer_T9 |
1191-1313 |
Sentence |
denotes |
Our data indicate that within 40 mum the breast cancer cells show significant polarization towards vessels in living mice. |
| T1 |
0-61 |
Sentence |
denotes |
Monomeric red fluorescent proteins with a large Stokes shift. |
| T2 |
62-158 |
Sentence |
denotes |
Two-photon microscopy has advanced fluorescence imaging of cellular processes in living animals. |
| T3 |
159-374 |
Sentence |
denotes |
Fluorescent proteins in the blue-green wavelength range are widely used in two-photon microscopy; however, the use of red fluorescent proteins is limited by the low power output of Ti-Sapphire lasers above 1,000 nm. |
| T4 |
375-584 |
Sentence |
denotes |
To overcome this limitation we have developed two red fluorescent proteins, LSS-mKate1 and LSS-mKate2, which possess large Stokes shifts with excitation/emission maxima at 463/624 and 460/605 nm, respectively. |
| T5 |
585-711 |
Sentence |
denotes |
These LSS-mKates are characterized by high pH stability, photostability, rapid chromophore maturation, and monomeric behavior. |
| T6 |
712-834 |
Sentence |
denotes |
They lack absorbance in the green region, providing an additional red color to the commonly used red fluorescent proteins. |
| T7 |
835-991 |
Sentence |
denotes |
Substantial overlap between the two-photon excitation spectra of the LSS-mKates and blue-green fluorophores enables multicolor imaging using a single laser. |
| T8 |
992-1190 |
Sentence |
denotes |
We applied this approach to a mouse xenograft model of breast cancer to intravitally study the motility and Golgi-nucleus alignment of tumor cells as a function of their distance from blood vessels. |
| T9 |
1191-1313 |
Sentence |
denotes |
Our data indicate that within 40 mum the breast cancer cells show significant polarization towards vessels in living mice. |