PubMed:20208171
Annnotations
pqqtest_sentence
{"project":"pqqtest_sentence","denotations":[{"id":"M_0","span":{"begin":82,"end":94},"obj":"xzyao:1553"},{"id":"M_1","span":{"begin":133,"end":145},"obj":"xzyao:1553"},{"id":"M_2","span":{"begin":536,"end":555},"obj":"xzyao:18145"},{"id":"M_3","span":{"begin":46,"end":72},"obj":"xzyao:22890"},{"id":"M_4","span":{"begin":1017,"end":1067},"obj":"xzyao:27051"},{"id":"M_5","span":{"begin":58,"end":72},"obj":"xzyao:31573"},{"id":"M_6","span":{"begin":163,"end":208},"obj":"xzyao:16404"},{"id":"M_7","span":{"begin":303,"end":319},"obj":"hunflair:NA:Species"},{"id":"M_8","span":{"begin":441,"end":498},"obj":"hunflair:NA:Chemical"},{"id":"M_9","span":{"begin":109,"end":125},"obj":"hunflair:NA:Gene"},{"id":"M_10","span":{"begin":192,"end":208},"obj":"hunflair:NA:Gene"},{"id":"M_11","span":{"begin":1041,"end":1057},"obj":"hunflair:NA:Gene"},{"id":"M_12","span":{"begin":599,"end":602},"obj":"hunflair:NA:Chemical"},{"id":"M_13","span":{"begin":163,"end":208},"obj":"hunflair:NA:Gene"},{"id":"M_14","span":{"begin":277,"end":288},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":536,"end":555},"obj":"hunflair:NA:Chemical"},{"id":"M_16","span":{"begin":618,"end":622},"obj":"hunflair:NA:Chemical"},{"id":"M_17","span":{"begin":500,"end":506},"obj":"hunflair:NA:Chemical"},{"id":"M_18","span":{"begin":127,"end":131},"obj":"hunflair:NA:Species"},{"id":"M_19","span":{"begin":82,"end":94},"obj":"hunflair:NA:Species"},{"id":"M_20","span":{"begin":133,"end":145},"obj":"hunflair:NA:Species"},{"id":"M_21","span":{"begin":594,"end":598},"obj":"hunflair:NA:Chemical"},{"id":"M_22","span":{"begin":210,"end":221},"obj":"hunflair:NA:Gene"},{"id":"M_23","span":{"begin":76,"end":80},"obj":"hunflair:NA:Species"},{"id":"M_24","span":{"begin":1023,"end":1029},"obj":"hunflair:NA:Species"},{"id":"M_25","span":{"begin":99,"end":108},"obj":"hunflair:NA:Gene"},{"id":"M_26","span":{"begin":150,"end":159},"obj":"hunflair:NA:Gene"},{"id":"M_27","span":{"begin":410,"end":419},"obj":"hunflair:NA:Gene"},{"id":"M_28","span":{"begin":922,"end":931},"obj":"hunflair:NA:Gene"},{"id":"M_29","span":{"begin":599,"end":602},"obj":"pubtator:MESH:C014843:Chemical"},{"id":"M_30","span":{"begin":303,"end":319},"obj":"pubtator:562:Species"},{"id":"M_31","span":{"begin":266,"end":267},"obj":"pubtator:MESH:D009584:Chemical"},{"id":"M_32","span":{"begin":387,"end":388},"obj":"pubtator:MESH:D009584:Chemical"},{"id":"M_33","span":{"begin":127,"end":131},"obj":"pubtator:4530:Species"},{"id":"M_34","span":{"begin":618,"end":622},"obj":"pubtator:MESH:D012965:Chemical"},{"id":"M_35","span":{"begin":82,"end":96},"obj":"pubtator:4530:Species"},{"id":"M_36","span":{"begin":133,"end":147},"obj":"pubtator:4530:Species"},{"id":"M_37","span":{"begin":536,"end":555},"obj":"pubtator:MESH:D011092:Chemical"},{"id":"M_38","span":{"begin":1017,"end":1029},"obj":"pubtator:3899:Species"},{"id":"M_39","span":{"begin":76,"end":80},"obj":"pubtator:4530:Species"},{"id":"M_40","span":{"begin":441,"end":498},"obj":"pubtator:MESH:C116347:Chemical"}],"text":"Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase.\nRice (Oryza sativa L.) Os4BGlu12, a glycoside hydrolase family 1 beta-glucosidase (EC 3.2.1.21), was expressed as a fusion protein with an N-terminal thioredoxin/His(6) tag in Escherichia coli strain Origami B (DE3) and purified with subsequent removal of the N-terminal tag. Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0.1 M Tris-HCl pH 8.5, 0.16 M NaCl at 288 K. Diffraction data sets for the apo and inhibitor-bound forms were collected to 2.50 and 2.45 A resolution, respectively. The space group and the unit-cell parameters of the crystal indicated the presence of two molecules per asymmetric unit, with a solvent content of 50%. The structure of Os4BGlu12 was successfully solved in space group P4(3)2(1)2 by molecular replacement using the white clover cyanogenic beta-glucosidase structure (PDB code 1cbg) as a search model."}
21k_plant_trait_mention
{"project":"21k_plant_trait_mention","denotations":[{"id":"M_0","span":{"begin":1017,"end":1029},"obj":"xzyao:13553"},{"id":"M_1","span":{"begin":1017,"end":1067},"obj":"xzyao:8679"},{"id":"M_2","span":{"begin":82,"end":94},"obj":"hunflair:NA:Species"},{"id":"M_3","span":{"begin":133,"end":145},"obj":"hunflair:NA:Species"},{"id":"M_4","span":{"begin":441,"end":498},"obj":"hunflair:NA:Chemical"},{"id":"M_5","span":{"begin":536,"end":555},"obj":"hunflair:NA:Chemical"},{"id":"M_6","span":{"begin":599,"end":602},"obj":"hunflair:NA:Chemical"},{"id":"M_7","span":{"begin":1023,"end":1029},"obj":"hunflair:NA:Species"},{"id":"M_8","span":{"begin":76,"end":80},"obj":"hunflair:NA:Species"},{"id":"M_9","span":{"begin":500,"end":506},"obj":"hunflair:NA:Chemical"},{"id":"M_10","span":{"begin":99,"end":108},"obj":"hunflair:NA:Gene"},{"id":"M_11","span":{"begin":150,"end":159},"obj":"hunflair:NA:Gene"},{"id":"M_12","span":{"begin":410,"end":419},"obj":"hunflair:NA:Gene"},{"id":"M_13","span":{"begin":922,"end":931},"obj":"hunflair:NA:Gene"},{"id":"M_14","span":{"begin":210,"end":221},"obj":"hunflair:NA:Gene"},{"id":"M_15","span":{"begin":618,"end":622},"obj":"hunflair:NA:Chemical"},{"id":"M_16","span":{"begin":303,"end":319},"obj":"hunflair:NA:Species"},{"id":"M_17","span":{"begin":127,"end":131},"obj":"hunflair:NA:Species"},{"id":"M_18","span":{"begin":109,"end":125},"obj":"hunflair:NA:Gene"},{"id":"M_19","span":{"begin":192,"end":208},"obj":"hunflair:NA:Gene"},{"id":"M_20","span":{"begin":1041,"end":1057},"obj":"hunflair:NA:Gene"},{"id":"M_21","span":{"begin":163,"end":208},"obj":"hunflair:NA:Gene"},{"id":"M_22","span":{"begin":594,"end":598},"obj":"hunflair:NA:Chemical"},{"id":"M_23","span":{"begin":277,"end":288},"obj":"hunflair:NA:Gene"},{"id":"M_24","span":{"begin":441,"end":498},"obj":"pubtator:MESH:C116347:Chemical"},{"id":"M_25","span":{"begin":1017,"end":1029},"obj":"pubtator:3899:Species"},{"id":"M_26","span":{"begin":599,"end":602},"obj":"pubtator:MESH:C014843:Chemical"},{"id":"M_27","span":{"begin":266,"end":267},"obj":"pubtator:MESH:D009584:Chemical"},{"id":"M_28","span":{"begin":387,"end":388},"obj":"pubtator:MESH:D009584:Chemical"},{"id":"M_29","span":{"begin":303,"end":319},"obj":"pubtator:562:Species"},{"id":"M_30","span":{"begin":76,"end":80},"obj":"pubtator:4530:Species"},{"id":"M_31","span":{"begin":536,"end":555},"obj":"pubtator:MESH:D011092:Chemical"},{"id":"M_32","span":{"begin":618,"end":622},"obj":"pubtator:MESH:D012965:Chemical"},{"id":"M_33","span":{"begin":127,"end":131},"obj":"pubtator:4530:Species"},{"id":"M_34","span":{"begin":82,"end":96},"obj":"pubtator:4530:Species"},{"id":"M_35","span":{"begin":133,"end":147},"obj":"pubtator:4530:Species"}],"text":"Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase.\nRice (Oryza sativa L.) Os4BGlu12, a glycoside hydrolase family 1 beta-glucosidase (EC 3.2.1.21), was expressed as a fusion protein with an N-terminal thioredoxin/His(6) tag in Escherichia coli strain Origami B (DE3) and purified with subsequent removal of the N-terminal tag. Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0.1 M Tris-HCl pH 8.5, 0.16 M NaCl at 288 K. Diffraction data sets for the apo and inhibitor-bound forms were collected to 2.50 and 2.45 A resolution, respectively. The space group and the unit-cell parameters of the crystal indicated the presence of two molecules per asymmetric unit, with a solvent content of 50%. The structure of Os4BGlu12 was successfully solved in space group P4(3)2(1)2 by molecular replacement using the white clover cyanogenic beta-glucosidase structure (PDB code 1cbg) as a search model."}
Allie
{"project":"Allie","denotations":[{"id":"SS1_20208171_2_0","span":{"begin":441,"end":498},"obj":"expanded"},{"id":"SS2_20208171_2_0","span":{"begin":500,"end":506},"obj":"abbr"}],"relations":[{"id":"AE1_20208171_2_0","pred":"abbreviatedTo","subj":"SS1_20208171_2_0","obj":"SS2_20208171_2_0"}],"text":"Expression, purification, crystallization and preliminary X-ray analysis of rice (Oryza sativa L.) Os4BGlu12 beta-glucosidase.\nRice (Oryza sativa L.) Os4BGlu12, a glycoside hydrolase family 1 beta-glucosidase (EC 3.2.1.21), was expressed as a fusion protein with an N-terminal thioredoxin/His(6) tag in Escherichia coli strain Origami B (DE3) and purified with subsequent removal of the N-terminal tag. Native Os4BGlu12 and its complex with 2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-glucopyranoside (DNP2FG) were crystallized using 19% polyethylene glycol (3350 or 2000, respectively) in 0.1 M Tris-HCl pH 8.5, 0.16 M NaCl at 288 K. Diffraction data sets for the apo and inhibitor-bound forms were collected to 2.50 and 2.45 A resolution, respectively. The space group and the unit-cell parameters of the crystal indicated the presence of two molecules per asymmetric unit, with a solvent content of 50%. The structure of Os4BGlu12 was successfully solved in space group P4(3)2(1)2 by molecular replacement using the white clover cyanogenic beta-glucosidase structure (PDB code 1cbg) as a search model."}