PubMed:20154331 JSONTXT

{"project":"SPECIES800","denotations":[{"id":"T1","span":{"begin":0,"end":40},"obj":"358766"},{"id":"T2","span":{"begin":170,"end":173},"obj":"358766"},{"id":"T3","span":{"begin":766,"end":769},"obj":"358766"},{"id":"T4","span":{"begin":1024,"end":1064},"obj":"358766"},{"id":"T5","span":{"begin":1084,"end":1104},"obj":"358766"},{"id":"T6","span":{"begin":1108,"end":1111},"obj":"358766"}],"namespaces":[{"prefix":"_base","uri":"http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info\u0026id="}],"text":"Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog.\nA novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T))."}