PubMed:20131264
Annnotations
PubMed_Structured_Abstracts
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 197-422 | OBJECTIVE | denotes | To examine the role of interleukin-23 (IL-23) in subgroup polarization of IL-17A-positive and/or interferon-gamma (IFNgamma)-positive T cells in autoimmune disease-prone DBA/1 mice with and without collagen-induced arthritis. |
| T2 | 432-927 | METHODS | denotes | A magnetic-activated cell sorting system was used to isolate CD4+ T cells from the spleen of naive and type II collagen (CII)-immunized DBA/1 mice. These CD4+ T cells were stimulated in vitro under Th0, Th1, or different Th17 culture conditions. Intracellular staining for IL-17A and IFNgamma was evaluated by flow cytometry. In addition, Th17 cytokines and T helper-specific transcription factors were analyzed by enzyme-linked immunosorbent assay and/or quantitative polymerase chain reaction. |
| T3 | 937-1885 | RESULTS | denotes | In CD4+ T cells from naive DBA/1 mice, IL-23 alone hardly induced retinoic acid-related orphan receptor gammat (RORgammat), Th17 polarization, and Th17 cytokines, but it inhibited T-bet expression. In contrast, transforming growth factor beta1 (TGFbeta1)/IL-6 was a potent inducer of RORgammat, RORalpha, IL-17A, IL-17F, IL-21, and FoxP3 in these cells. In contrast to TGFbeta1/IL-6, IL-23 was critical for the induction of IL-22 in CD4+ T cells from both naive and CII-immunized DBA/1 mice. Consistent with these findings, IL-23 showed a more pronounced induction of the IL-17A+IFNgamma- subset in CD4+ T cells from CII-immunized mice. However, in CD4+ T cells from naive mice, IL-23 significantly increased the TGFbeta1/IL-6-induced Th17 polarization, including elevated levels of IL-17A and IL-17F and decreased expression of T-bet and FoxP3. Of note, the IL-23-induced increase in IL-17A and IL-17F levels was prevented in T-bet-deficient mice. |
| T4 | 1898-2219 | CONCLUSIONS | denotes | IL-23 promotes Th17 differentiation by inhibiting T-bet and FoxP3 and is required for elevation of IL-22, but not IL-21, levels in autoimmune arthritis. These data indicate different mechanisms for IL-23 and TGFbeta1/IL-6 at the transcription factor level during Th17 differentiation in autoimmune experimental arthritis. |
PubmedHPO
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 342-360 | HP_0002960 | denotes | autoimmune disease |
| T2 | 342-352 | HP_0002960 | denotes | autoimmune |
| T3 | 412-421 | HP_0001369 | denotes | arthritis |
Allie
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| SS1_20131264_2_0 | 220-234 | expanded | denotes | interleukin-23 |
| SS2_20131264_2_0 | 236-241 | abbr | denotes | IL-23 |
| SS1_20131264_2_1 | 294-310 | expanded | denotes | interferon-gamma |
| SS2_20131264_2_1 | 312-320 | abbr | denotes | IFNgamma |
| SS1_20131264_4_0 | 500-551 | expanded | denotes | cells from the spleen of naive and type II collagen |
| SS2_20131264_4_0 | 553-556 | abbr | denotes | CII |
| SS1_20131264_9_0 | 1012-1047 | expanded | denotes | acid-related orphan receptor gammat |
| SS2_20131264_9_0 | 1049-1058 | abbr | denotes | RORgammat |
| SS1_20131264_10_0 | 1148-1180 | expanded | denotes | transforming growth factor beta1 |
| SS2_20131264_10_0 | 1182-1190 | abbr | denotes | TGFbeta1 |
| AE1_20131264_2_0 | SS1_20131264_2_0 | SS2_20131264_2_0 | abbreviatedTo | interleukin-23,IL-23 |
| AE1_20131264_2_1 | SS1_20131264_2_1 | SS2_20131264_2_1 | abbreviatedTo | interferon-gamma,IFNgamma |
| AE1_20131264_4_0 | SS1_20131264_4_0 | SS2_20131264_4_0 | abbreviatedTo | cells from the spleen of naive and type II collagen,CII |
| AE1_20131264_9_0 | SS1_20131264_9_0 | SS2_20131264_9_0 | abbreviatedTo | acid-related orphan receptor gammat,RORgammat |
| AE1_20131264_10_0 | SS1_20131264_10_0 | SS2_20131264_10_0 | abbreviatedTo | transforming growth factor beta1,TGFbeta1 |
2015-BEL-Sample-2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| BEL:20034538 | 1574-1884 | complex(p(MGI:Il12b),p(MGI:Il23a)) decreases r(MGI:Foxp3) | denotes | However, in CD4+ T cells from naive mice, IL-23 significantly increased the TGFbeta1/IL-6-induced Th17 polarization, including elevated levels of IL-17A and IL-17F and decreased expression of T-bet and FoxP3. Of note, the IL-23-induced increase in IL-17A and IL-17F levels was prevented in T-bet-deficient mice |
| BEL:20034542 | 1574-1884 | complex(p(MGI:Il12b),p(MGI:Il23a)) increases r(MGI:Il17a) | denotes | However, in CD4+ T cells from naive mice, IL-23 significantly increased the TGFbeta1/IL-6-induced Th17 polarization, including elevated levels of IL-17A and IL-17F and decreased expression of T-bet and FoxP3. Of note, the IL-23-induced increase in IL-17A and IL-17F levels was prevented in T-bet-deficient mice |
| BEL:20034546 | 1574-1884 | complex(p(MGI:Il12b),p(MGI:Il23a)) increases r(MGI:Il17f) | denotes | However, in CD4+ T cells from naive mice, IL-23 significantly increased the TGFbeta1/IL-6-induced Th17 polarization, including elevated levels of IL-17A and IL-17F and decreased expression of T-bet and FoxP3. Of note, the IL-23-induced increase in IL-17A and IL-17F levels was prevented in T-bet-deficient mice |
| BEL:20034550 | 1291-1427 | complex(p(MGI:Il12b),p(MGI:Il23a)) increases r(MGI:Il22) | denotes | In contrast to TGFbeta1/IL-6, IL-23 was critical for the induction of IL-22 in CD4+ T cells from both naive and CII-immunized DBA/1 mice |
| BEL:20034564 | 935-1133 | complex(p(MGI:Il23a),p(MGI:Il1b)) decreases r(MGI:Tbx21) | denotes | : In CD4+ T cells from naive DBA/1 mice, IL-23 alone hardly induced retinoic acid-related orphan receptor gammat (RORgammat), Th17 polarization, and Th17 cytokines, but it inhibited T-bet expression |