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PubMed:20122994 JSONTXT

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Allie

Id Subject Object Predicate Lexical cue
SS1_20122994_2_0 186-210 expanded denotes Agrocybe aegerita lectin
SS2_20122994_2_0 212-215 abbr denotes AAL
SS1_20122994_6_0 510-541 expanded denotes carbohydrate recognition domain
SS2_20122994_6_0 543-546 abbr denotes CRD
SS1_20122994_7_0 696-711 expanded denotes recombinant AAL
SS2_20122994_7_0 713-717 abbr denotes rAAL
SS1_20122994_13_0 1218-1246 expanded denotes glucose-regulated protein 78
SS2_20122994_13_0 1248-1253 abbr denotes GRP78
SS1_20122994_13_1 1331-1352 expanded denotes heat shock protein 70
SS2_20122994_13_1 1354-1359 abbr denotes Hsp70
AE1_20122994_2_0 SS1_20122994_2_0 SS2_20122994_2_0 abbreviatedTo Agrocybe aegerita lectin,AAL
AE1_20122994_6_0 SS1_20122994_6_0 SS2_20122994_6_0 abbreviatedTo carbohydrate recognition domain,CRD
AE1_20122994_7_0 SS1_20122994_7_0 SS2_20122994_7_0 abbreviatedTo recombinant AAL,rAAL
AE1_20122994_13_0 SS1_20122994_13_0 SS2_20122994_13_0 abbreviatedTo glucose-regulated protein 78,GRP78
AE1_20122994_13_1 SS1_20122994_13_1 SS2_20122994_13_1 abbreviatedTo heat shock protein 70,Hsp70

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 744-748 Disease denotes FACS http://purl.obolibrary.org/obo/MONDO_0018262

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 1069-1076 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 1336-1341 Phenotype denotes shock HP:0031273

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 744-748 Disease denotes FACS http://purl.obolibrary.org/obo/MONDO_0018262

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 95-112 OrganismTaxon denotes Agrocybe aegerita 1973307
T2 186-203 OrganismTaxon denotes Agrocybe aegerita 1973307

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 663-676 Body_part denotes cell membrane http://purl.obolibrary.org/obo/GO_0005886
T2 1110-1123 Body_part denotes cell membrane http://purl.obolibrary.org/obo/GO_0005886

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-121 Sentence denotes A nuclear ligand MRG15 involved in the proapoptotic activity of medicinal fungal galectin AAL (Agrocybe aegerita lectin).
T2 122-133 Sentence denotes BACKGROUND:
T3 134-280 Sentence denotes We have previously reported a novel fungal galectin Agrocybe aegerita lectin (AAL) with apoptosis-induced activity and nuclear migration activity.
T4 281-394 Sentence denotes The importance of nuclear localization for AAL's apoptosis-induced activity has been established by mutant study.
T5 395-434 Sentence denotes However, the mechanism remains unclear.
T6 435-443 Sentence denotes METHODS:
T7 444-658 Sentence denotes We further investigated the mechanism using a previously reported carbohydrate recognition domain (CRD) mutant protein H59Q, which retained its nuclear localization activity but lost most of its apoptotic activity.
T8 659-843 Sentence denotes The cell membrane-binding ability of recombinant AAL (rAAL) and H59Q was analyzed by FACS, and their cellular partners were identified by affinity chromatography and mass spectroscopy.
T9 844-947 Sentence denotes Furthermore, the interaction of AAL and ligand was proved by mammalian two-hybrid and pull down assays.
T10 948-1009 Sentence denotes A knockdown assay was used to confirm the role of the ligand.
T11 1010-1018 Sentence denotes RESULTS:
T12 1019-1077 Sentence denotes The apoptotic activity of AAL could be blocked by lactose.
T13 1078-1148 Sentence denotes Mutant H59Q retained comparable cell membrane-binding ability to rAAL.
T14 1149-1361 Sentence denotes Four cellular binding partners of AAL in HeLa cells were identified: glucose-regulated protein 78 (GRP78); mortality factor 4-like protein 1 (MRG15); elongation factor 2 (EEF2); and heat shock protein 70 (Hsp70).
T15 1362-1446 Sentence denotes CRD region of AAL was required for the interaction between AAL/mutant AAL and MRG15.
T16 1447-1512 Sentence denotes MRG15 knockdown increased the cells' resistance to AAL treatment.
T17 1513-1574 Sentence denotes CONCLUSION: MRG15 was a nuclear ligand for AAL in HeLa cells.
T18 1575-1685 Sentence denotes These data implied the existence of a novel nuclear pathway for the antitumor activity of fungal galectin AAL.
T19 1686-1707 Sentence denotes GENERAL SIGNIFICANCE:
T20 1708-1846 Sentence denotes These findings provide a novel explanation of AAL bioactivity and contribute to the understanding of mushroom lectins' antitumor activity.

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 1069-1076 Glycan denotes lactose https://api.glycosmos.org/wurcs2image/latest/png/binary/G15541SE

GlyCosmos15-Lectin-Jamboree

Id Subject Object Predicate Lexical cue glycosmos_id
T1 90-93 Lectin denotes AAL GL_001404
T2 212-215 Lectin denotes AAL GL_001404
T3 708-711 Lectin denotes AAL GL_001404
T4 876-879 Lectin denotes AAL GL_001404
T5 1045-1048 Lectin denotes AAL GL_001404
T6 1183-1186 Lectin denotes AAL GL_001404
T7 1376-1379 Lectin denotes AAL GL_001404
T8 1421-1424 Lectin denotes AAL GL_001404
T9 1432-1435 Lectin denotes AAL GL_001404
T10 1498-1501 Lectin denotes AAL GL_001404
T11 1556-1559 Lectin denotes AAL GL_001404
T12 1681-1684 Lectin denotes AAL GL_001404
T13 1754-1757 Lectin denotes AAL GL_001404

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 95-112 OrganismTaxon denotes Agrocybe aegerita 1973307
T2 186-203 OrganismTaxon denotes Agrocybe aegerita 1973307

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 663-676 Body_part denotes cell membrane http://purl.obolibrary.org/obo/GO_0005886
T2 1110-1123 Body_part denotes cell membrane http://purl.obolibrary.org/obo/GO_0005886

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 1336-1341 Phenotype denotes shock HP:0031273