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PubMed:20110369 JSONTXT

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sentences

Id Subject Object Predicate Lexical cue
T1 0-171 Sentence denotes Direct interaction between quorum-sensing regulator SmcR and RNA polymerase is mediated by integration host factor to activate vvpE encoding elastase in Vibrio vulnificus.
T2 172-334 Sentence denotes It has been suggested that quorum sensing is an important signal transduction system regulating the expression of numerous virulence genes in bacterial pathogens.
T3 335-568 Sentence denotes We previously revealed that SmcR, a LuxR homologue of Vibrio vulnificus, activates promoter S, an RpoS-dependent promoter of vvpE encoding a potential virulence factor elastase and binds in vitro to a binding site centered at -196.5.
T4 569-790 Sentence denotes In this study, chromatin immunoprecipitation assays and promoter deletion analyses demonstrated that SmcR binds to the vvpE regulatory region in vivo and directly interacts with RNAP for activation of the vvpE expression.
T5 791-950 Sentence denotes A search for regulatory genes involved in the regulation of elastase production singled out ihfA, which encodes for a subunit of integration host factor (IHF).
T6 951-1167 Sentence denotes Levels of both elastase activity and vvpE transcript decreased significantly as a result of inactivation of ihfA, and primer extension analyses demonstrated that IHF regulates the vvpE transcription by activating PS.
T7 1168-1357 Sentence denotes Direct binding of IHF to the two distinct binding sites centered at -174 and -131, respectively, was determined using an electrophoretic mobility shift assay and a DNase I protection assay.
T8 1358-1471 Sentence denotes Chromatin immunoprecipitation assays revealed that the interaction of SmcR with RNAP in vivo was mediated by IHF.
T9 1472-1697 Sentence denotes Collectively, the results proposed a model whereby IHF positions SmcR to contact RNAP by looping the vvpE regulatory DNA, thus allowing precise control of the expression level of VvpE during the pathogenesis of V. vulnificus.

Allie

Id Subject Object Predicate Lexical cue
SS1_20110369_4_0 920-943 expanded denotes integration host factor
SS2_20110369_4_0 945-948 abbr denotes IHF
AE1_20110369_4_0 SS1_20110369_4_0 SS2_20110369_4_0 abbreviatedTo integration host factor,IHF

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 945-948 Disease denotes IHF http://purl.obolibrary.org/obo/MONDO_0018221
T2 1113-1116 Disease denotes IHF http://purl.obolibrary.org/obo/MONDO_0018221
T3 1186-1189 Disease denotes IHF http://purl.obolibrary.org/obo/MONDO_0018221
T4 1467-1470 Disease denotes IHF http://purl.obolibrary.org/obo/MONDO_0018221
T5 1523-1526 Disease denotes IHF http://purl.obolibrary.org/obo/MONDO_0018221

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 153-170 OrganismTaxon denotes Vibrio vulnificus 672
T2 389-406 OrganismTaxon denotes Vibrio vulnificus 672

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 1076-1085 Body_part denotes extension http://purl.obolibrary.org/obo/UBERON_2000106