> top > docs > PubMed:199739 > annotations

PubMed:199739 JSONTXT

Annnotations TAB JSON ListView MergeView

Oryza_sentences

Id Subject Object Predicate Lexical cue
T1 0-69 Sentence denotes Recombination between endogenous and exogenous simian virus 40 genes.
T2 70-180 Sentence denotes I. Rescue of a simian virus 40 temperature-sensitive mutant by passage in permissive transformed monkey lines.
T3 181-505 Sentence denotes Passage of the simian virus 40 (SV40) temperature-sensitive (ts) mutant tsD202 at the permissive temperature in each of three permissive lines of SV40-transformed monkey CV1 cells resulted in the emergence of temperature-insensitive virus, which plated like wild-type SV40 at the restrictive temperature on normal CV1 cells.
T4 506-779 Sentence denotes In independent experiments, the amount of temperature-insensitive virus that appeared after passage on transformed cells was from 10(3)- to 10(6)-fold greater than the amount of ts-revertant virus that appeared after an equal number of passages in nontransformed CV1 cells.
T5 780-1060 Sentence denotes The virus rescued by passage on transformed cells bred true upon sequential plaque purification, plated on normal CV1 cells with single-hit kinetics at the restrictive temperature, and displayed no selective growth advantage on transformed cells compared to non-transformed cells.
T6 1061-1231 Sentence denotes Hence, the reversion of the ts phenotype is neither due to complementation effects nor to the selection of preexisting revertants, which grow better on transformed cells.
T7 1232-1460 Sentence denotes In the accompanying article (T. Vogel et al., J. Virol. 24:541-550, 1977), we present biochemical evidence that the rescue of tsD202 mediated by passage on transformed cells is due to recombination with the resident SV40 genome.
T8 1461-1719 Sentence denotes Parallel experiments in which tsA, tsB, and tsC SV40 mutants were passaged in each of the three permissive lines of SV40-transformed monkey cells resulted in either only borderline levels of rescue (tsA mutants) or no detectable rescue (tsB and tsC mutants).
T9 1720-1927 Sentence denotes Evidence is presented that the resident SV40 genome of the transformed monkey lines is itself a late ts mutant, and we suggest that this accounts for the lack of detectable rescue of the tsB and tsC mutants.
T10 1928-2263 Sentence denotes We furthermore suggest that the borderline level of rescue observed with two tsA mutants is related to a previous finding (Y. Gluzman et al., J. Virol. 22:256-266, 1977) which indicated that the resident SV40 genome of the permissive transformed monkey cells is defective in the function required for initiation of viral DNA synthesis.