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sentences

Id Subject Object Predicate Lexical cue
T1 0-154 Sentence denotes The recognition unit of FIBCD1 organizes into a noncovalently linked tetrameric structure and uses a hydrophobic funnel (S1) for acetyl group recognition.
T2 155-320 Sentence denotes We have recently identified FIBCD1 (Fibrinogen C domain containing 1) as a type II transmembrane endocytic receptor located primarily in the intestinal brush border.
T3 321-495 Sentence denotes The ectodomain of FIBCD1 comprises a coiled coil, a polycationic region, and a C-terminal FReD (fibrinogen-related domain) that assembles into disulfide-linked homotetramers.
T4 496-619 Sentence denotes The FIBCD1-FReD binds Ca(2+) dependently to acetylated structures like chitin, N-acetylated carbohydrates, and amino acids.
T5 620-739 Sentence denotes FReDs are present in diverse innate immune pattern recognition proteins including the ficolins and horseshoe crab TL5A.
T6 740-983 Sentence denotes Here, we use chemical cross-linking, combined with analytical ultracentrifugation and electron microscopy of the negatively stained recombinant FIBCD1-FReD to show that it assembles into noncovalent tetramers in the absence of the coiled coil.
T7 984-1192 Sentence denotes We use surface plasmon resonance, carbohydrate binding, and pulldown assays combined with site-directed mutagenesis to define the binding site involved in the interaction of FIBCD1 with acetylated structures.
T8 1193-1361 Sentence denotes We show that mutations of central residues (A432V and H415G) in the hydrophobic funnel (S1) abolish the binding of FIBCD1 to acetylated bovine serum albumin and chitin.
T9 1362-1479 Sentence denotes The double mutations (D393N/D395A) at the putative calcium-binding site reduce the ability of FIBCD1 to bind ligands.
T10 1480-1684 Sentence denotes We conclude that the FReDs of FIBCD1 forms noncovalent tetramers and that the acetyl-binding site of FReDs of FIBCD1 is homologous to that of tachylectin 5A and M-ficolin but not to the FReD of L-ficolin.
T11 1685-1838 Sentence denotes We suggest that the spatial organization of the FIBCD1-FReDs determine the molecular pattern recognition specificity and subsequent biological functions.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 296-306 http://purl.obolibrary.org/obo/MAT_0000043 denotes intestinal

Allie

Id Subject Object Predicate Lexical cue
SS1_19892701_1_0 191-223 expanded denotes Fibrinogen C domain containing 1
SS2_19892701_1_0 183-189 abbr denotes FIBCD1
AE1_19892701_1_0 SS1_19892701_1_0 SS2_19892701_1_0 abbreviatedTo Fibrinogen C domain containing 1,FIBCD1

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 567-573 Glycan denotes chitin
T2 1354-1360 Glycan denotes chitin

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 296-306 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 567-573 Glycan denotes chitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G97099AY
T2 1354-1360 Glycan denotes chitin https://api.glycosmos.org/wurcs2image/latest/png/binary/G97099AY

GlyCosmos15-NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1329-1335 OrganismTaxon denotes bovine 9913

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 238-251 Body_part denotes transmembrane http://purl.obolibrary.org/obo/GO_0016020
T2 296-319 Body_part denotes intestinal brush border http://purl.obolibrary.org/obo/UBERON_0012427

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-154 Sentence denotes The recognition unit of FIBCD1 organizes into a noncovalently linked tetrameric structure and uses a hydrophobic funnel (S1) for acetyl group recognition.
T2 155-320 Sentence denotes We have recently identified FIBCD1 (Fibrinogen C domain containing 1) as a type II transmembrane endocytic receptor located primarily in the intestinal brush border.
T3 321-495 Sentence denotes The ectodomain of FIBCD1 comprises a coiled coil, a polycationic region, and a C-terminal FReD (fibrinogen-related domain) that assembles into disulfide-linked homotetramers.
T4 496-619 Sentence denotes The FIBCD1-FReD binds Ca(2+) dependently to acetylated structures like chitin, N-acetylated carbohydrates, and amino acids.
T5 620-739 Sentence denotes FReDs are present in diverse innate immune pattern recognition proteins including the ficolins and horseshoe crab TL5A.
T6 740-983 Sentence denotes Here, we use chemical cross-linking, combined with analytical ultracentrifugation and electron microscopy of the negatively stained recombinant FIBCD1-FReD to show that it assembles into noncovalent tetramers in the absence of the coiled coil.
T7 984-1192 Sentence denotes We use surface plasmon resonance, carbohydrate binding, and pulldown assays combined with site-directed mutagenesis to define the binding site involved in the interaction of FIBCD1 with acetylated structures.
T8 1193-1361 Sentence denotes We show that mutations of central residues (A432V and H415G) in the hydrophobic funnel (S1) abolish the binding of FIBCD1 to acetylated bovine serum albumin and chitin.
T9 1362-1479 Sentence denotes The double mutations (D393N/D395A) at the putative calcium-binding site reduce the ability of FIBCD1 to bind ligands.
T10 1480-1684 Sentence denotes We conclude that the FReDs of FIBCD1 forms noncovalent tetramers and that the acetyl-binding site of FReDs of FIBCD1 is homologous to that of tachylectin 5A and M-ficolin but not to the FReD of L-ficolin.
T11 1685-1838 Sentence denotes We suggest that the spatial organization of the FIBCD1-FReDs determine the molecular pattern recognition specificity and subsequent biological functions.

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 296-306 Body_part denotes intestinal FMA:7199
T2 307-319 Body_part denotes brush border FMA:70977
T3 1336-1341 Body_part denotes serum FMA:63083

GlyCosmos15-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 296-306 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 1329-1335 OrganismTaxon denotes bovine 9913

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 238-251 Body_part denotes transmembrane http://purl.obolibrary.org/obo/GO_0016020
T2 296-319 Body_part denotes intestinal brush border http://purl.obolibrary.org/obo/UBERON_0012427