PubMed:19850049 JSONTXT

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":1453,"end":1458},"obj":"gene:3479"},{"id":"T1","span":{"begin":1545,"end":1552},"obj":"disease:C0856742"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"Analysis of angiogenesis induced by local IGF-1 expression after myocardial infarction using microSPECT-CT imaging.\nInsulin-like growth factor-1 (IGF-1) has been found to exert favorable effects on angiogenesis in prior animal studies. This study explored the long-term effect of IGF-1 on angiogenesis using microSPECT-CT in infarcted rat hearts after delivering human IGF-1 gene by adeno-associated virus (AAV). Myocardial infarction (MI) was induced in Sprague-Dawley rats by ligation of the proximal anterior coronary artery and a total of 10(11) AAV-CMV-lacZ (control) or IGF-1 vectors were injected around the peri-infarct area. IGF-1 expression by AAV stably transduced heart muscle for up to 16 weeks post-MI and immunohistochemistry revealed a remarkable increase in capillary density. A (99m)Tc-labeled RGD peptide (NC100692, GE Healthcare) was used to assess temporal and regional alpha(v) integrin activation. Rats were injected with NC100692 followed by (201)Tl chloride and in vivo microSPECT-CT imaging was performed. After imaging, hearts were excised and cut for quantitative gamma-well counting (GWC). NC100692 retention was significantly increased in hypoperfused regions of both lacZ and IGF-1 rats at 4 and 16 weeks post-MI. Significantly higher activation of alpha(v) integrin was observed in IGF-1 rats at 4 weeks after treatment compared with control group, although the activation was lower in the IGF-1 group at 16 weeks. Local IGF-1 gene delivery by AAV can render a sustained transduction and improve cardiac function post-MI. IGF-1 expression contributes to enhanced alpha(v) integrin activation which is linked to angiogenesis. MicroSPECT-CT imaging with (99m)Tc-NC100692 and quantitative GWC successfully assessed differences in alpha(v) integrin activation between IGF-1-treated and control animals post-MI."}

{"project":"Allie","denotations":[{"id":"SS1_19850049_1_0","span":{"begin":116,"end":144},"obj":"expanded"},{"id":"SS2_19850049_1_0","span":{"begin":146,"end":151},"obj":"abbr"},{"id":"SS1_19850049_2_0","span":{"begin":383,"end":405},"obj":"expanded"},{"id":"SS2_19850049_2_0","span":{"begin":407,"end":410},"obj":"abbr"},{"id":"SS1_19850049_3_0","span":{"begin":413,"end":434},"obj":"expanded"},{"id":"SS2_19850049_3_0","span":{"begin":436,"end":438},"obj":"abbr"},{"id":"SS1_19850049_7_0","span":{"begin":1092,"end":1111},"obj":"expanded"},{"id":"SS2_19850049_7_0","span":{"begin":1113,"end":1116},"obj":"abbr"}],"relations":[{"id":"AE1_19850049_1_0","pred":"abbreviatedTo","subj":"SS1_19850049_1_0","obj":"SS2_19850049_1_0"},{"id":"AE1_19850049_2_0","pred":"abbreviatedTo","subj":"SS1_19850049_2_0","obj":"SS2_19850049_2_0"},{"id":"AE1_19850049_3_0","pred":"abbreviatedTo","subj":"SS1_19850049_3_0","obj":"SS2_19850049_3_0"},{"id":"AE1_19850049_7_0","pred":"abbreviatedTo","subj":"SS1_19850049_7_0","obj":"SS2_19850049_7_0"}],"text":"Analysis of angiogenesis induced by local IGF-1 expression after myocardial infarction using microSPECT-CT imaging.\nInsulin-like growth factor-1 (IGF-1) has been found to exert favorable effects on angiogenesis in prior animal studies. This study explored the long-term effect of IGF-1 on angiogenesis using microSPECT-CT in infarcted rat hearts after delivering human IGF-1 gene by adeno-associated virus (AAV). Myocardial infarction (MI) was induced in Sprague-Dawley rats by ligation of the proximal anterior coronary artery and a total of 10(11) AAV-CMV-lacZ (control) or IGF-1 vectors were injected around the peri-infarct area. IGF-1 expression by AAV stably transduced heart muscle for up to 16 weeks post-MI and immunohistochemistry revealed a remarkable increase in capillary density. A (99m)Tc-labeled RGD peptide (NC100692, GE Healthcare) was used to assess temporal and regional alpha(v) integrin activation. Rats were injected with NC100692 followed by (201)Tl chloride and in vivo microSPECT-CT imaging was performed. After imaging, hearts were excised and cut for quantitative gamma-well counting (GWC). NC100692 retention was significantly increased in hypoperfused regions of both lacZ and IGF-1 rats at 4 and 16 weeks post-MI. Significantly higher activation of alpha(v) integrin was observed in IGF-1 rats at 4 weeks after treatment compared with control group, although the activation was lower in the IGF-1 group at 16 weeks. Local IGF-1 gene delivery by AAV can render a sustained transduction and improve cardiac function post-MI. IGF-1 expression contributes to enhanced alpha(v) integrin activation which is linked to angiogenesis. MicroSPECT-CT imaging with (99m)Tc-NC100692 and quantitative GWC successfully assessed differences in alpha(v) integrin activation between IGF-1-treated and control animals post-MI."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"19850049-0#42#47#gene3479","span":{"begin":42,"end":47},"obj":"gene3479"},{"id":"19850049-0#65#86#diseaseC0027051","span":{"begin":65,"end":86},"obj":"diseaseC0027051"},{"id":"19850049-10#6#11#gene3479","span":{"begin":1453,"end":1458},"obj":"gene3479"},{"id":"19850049-10#98#105#diseaseC0856742","span":{"begin":1545,"end":1552},"obj":"diseaseC0856742"}],"relations":[{"id":"42#47#gene347965#86#diseaseC0027051","pred":"associated_with","subj":"19850049-0#42#47#gene3479","obj":"19850049-0#65#86#diseaseC0027051"},{"id":"6#11#gene347998#105#diseaseC0856742","pred":"associated_with","subj":"19850049-10#6#11#gene3479","obj":"19850049-10#98#105#diseaseC0856742"}],"text":"Analysis of angiogenesis induced by local IGF-1 expression after myocardial infarction using microSPECT-CT imaging.\nInsulin-like growth factor-1 (IGF-1) has been found to exert favorable effects on angiogenesis in prior animal studies. This study explored the long-term effect of IGF-1 on angiogenesis using microSPECT-CT in infarcted rat hearts after delivering human IGF-1 gene by adeno-associated virus (AAV). Myocardial infarction (MI) was induced in Sprague-Dawley rats by ligation of the proximal anterior coronary artery and a total of 10(11) AAV-CMV-lacZ (control) or IGF-1 vectors were injected around the peri-infarct area. IGF-1 expression by AAV stably transduced heart muscle for up to 16 weeks post-MI and immunohistochemistry revealed a remarkable increase in capillary density. A (99m)Tc-labeled RGD peptide (NC100692, GE Healthcare) was used to assess temporal and regional alpha(v) integrin activation. Rats were injected with NC100692 followed by (201)Tl chloride and in vivo microSPECT-CT imaging was performed. After imaging, hearts were excised and cut for quantitative gamma-well counting (GWC). NC100692 retention was significantly increased in hypoperfused regions of both lacZ and IGF-1 rats at 4 and 16 weeks post-MI. Significantly higher activation of alpha(v) integrin was observed in IGF-1 rats at 4 weeks after treatment compared with control group, although the activation was lower in the IGF-1 group at 16 weeks. Local IGF-1 gene delivery by AAV can render a sustained transduction and improve cardiac function post-MI. IGF-1 expression contributes to enhanced alpha(v) integrin activation which is linked to angiogenesis. MicroSPECT-CT imaging with (99m)Tc-NC100692 and quantitative GWC successfully assessed differences in alpha(v) integrin activation between IGF-1-treated and control animals post-MI."}