PubMed:1983782 / 496-719 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T4","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"T4","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"T4","span":{"begin":0,"end":223},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T35","span":{"begin":45,"end":54},"obj":"FMAID:82765"},{"id":"_T36","span":{"begin":45,"end":54},"obj":"FMAID:196754"},{"id":"_T37","span":{"begin":69,"end":76},"obj":"FMAID:82749"},{"id":"_T38","span":{"begin":69,"end":76},"obj":"FMAID:196738"},{"id":"_T39","span":{"begin":102,"end":110},"obj":"FMAID:165447"},{"id":"_T40","span":{"begin":102,"end":110},"obj":"FMAID:67257"},{"id":"_T41","span":{"begin":130,"end":135},"obj":"FMAID:169002"},{"id":"_T42","span":{"begin":130,"end":135},"obj":"FMAID:68646"},{"id":"_T43","span":{"begin":176,"end":178},"obj":"FMAID:63509"},{"id":"_T44","span":{"begin":176,"end":178},"obj":"FMAID:167737"},{"id":"_T45","span":{"begin":218,"end":222},"obj":"FMAID:198663"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T6","span":{"begin":130,"end":135},"obj":"http://purl.bioontology.org/ontology/STY/T025"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}

{"project":"GO-CC","denotations":[{"id":"T6","span":{"begin":130,"end":135},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}

{"project":"EDAM-topics","denotations":[{"id":"T8","span":{"begin":102,"end":110},"obj":"http://edamontology.org/topic_0078"},{"id":"T9","span":{"begin":179,"end":187},"obj":"http://edamontology.org/topic_0749"},{"id":"T10","span":{"begin":179,"end":187},"obj":"http://edamontology.org/topic_1312"},{"id":"T11","span":{"begin":179,"end":187},"obj":"http://edamontology.org/topic_0111"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}

{"project":"EDAM-DFO","denotations":[{"id":"T9","span":{"begin":55,"end":63},"obj":"http://edamontology.org/data_1756"},{"id":"T10","span":{"begin":102,"end":110},"obj":"http://edamontology.org/format_1208"},{"id":"T11","span":{"begin":102,"end":110},"obj":"http://edamontology.org/data_1467"}],"text":"We have altered these sites by replacing the threonine residues with alanine and expressed the mutant proteins transiently in CV1 cells utilizing a mutagenesis vector with the T7 promoter located upstream from the PrP gene."}