PubMed:19836396 JSONTXT

{"project":"silkworm_phenotype","denotations":[{"id":"T28","span":{"begin":725,"end":730},"obj":"BMO_00479"},{"id":"T27","span":{"begin":668,"end":673},"obj":"BMO_00480"},{"id":"T26","span":{"begin":636,"end":641},"obj":"BMO_00480"},{"id":"T24","span":{"begin":622,"end":627},"obj":"BMO_00479"},{"id":"T22","span":{"begin":575,"end":580},"obj":"BMO_00479"},{"id":"T21","span":{"begin":523,"end":528},"obj":"BMO_00480"},{"id":"T19","span":{"begin":468,"end":473},"obj":"BMO_00479"},{"id":"T18","span":{"begin":458,"end":463},"obj":"BMO_00480"},{"id":"T13","span":{"begin":298,"end":303},"obj":"BMO_00479"},{"id":"T12","span":{"begin":288,"end":293},"obj":"BMO_00480"},{"id":"T11","span":{"begin":64,"end":69},"obj":"BMO_00480"},{"id":"T9","span":{"begin":54,"end":59},"obj":"BMO_00479"},{"id":"T8","span":{"begin":832,"end":847},"obj":"Disease:MESH:D001102"},{"id":"T7","span":{"begin":695,"end":704},"obj":"Disease:MESH:D007239"},{"id":"T6","span":{"begin":421,"end":430},"obj":"Species:7091"},{"id":"T5","span":{"begin":223,"end":236},"obj":"Disease:MESH:D034062"},{"id":"T4","span":{"begin":141,"end":164},"obj":"Species:10809"},{"id":"T3","span":{"begin":132,"end":140},"obj":"Species:7091"},{"id":"T2","span":{"begin":100,"end":115},"obj":"Disease:MESH:D001102"},{"id":"T1","span":{"begin":28,"end":45},"obj":"Species:10809"}],"text":"Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway.\nIn the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":28,"end":45},"obj":"Species:10809"},{"id":"T2","span":{"begin":100,"end":115},"obj":"Disease:MESH:D001102"},{"id":"T3","span":{"begin":132,"end":140},"obj":"Species:7091"},{"id":"T4","span":{"begin":141,"end":164},"obj":"Species:10809"},{"id":"T5","span":{"begin":223,"end":236},"obj":"Disease:MESH:D034062"},{"id":"T6","span":{"begin":421,"end":430},"obj":"Species:7091"},{"id":"T7","span":{"begin":695,"end":704},"obj":"Disease:MESH:D007239"},{"id":"T8","span":{"begin":832,"end":847},"obj":"Disease:MESH:D001102"}],"text":"Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway.\nIn the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":28,"end":45},"obj":"Species:10809"},{"id":"T2","span":{"begin":100,"end":115},"obj":"Disease:MESH:D001102"},{"id":"T3","span":{"begin":132,"end":140},"obj":"Species:7091"},{"id":"T4","span":{"begin":141,"end":164},"obj":"Species:10809"},{"id":"T5","span":{"begin":223,"end":236},"obj":"Disease:MESH:D034062"},{"id":"T6","span":{"begin":421,"end":430},"obj":"Species:7091"},{"id":"T7","span":{"begin":695,"end":704},"obj":"Disease:MESH:D007239"},{"id":"T8","span":{"begin":832,"end":847},"obj":"Disease:MESH:D001102"},{"id":"T9","span":{"begin":54,"end":59},"obj":"BMO_00479"},{"id":"T11","span":{"begin":64,"end":69},"obj":"BMO_00480"},{"id":"T12","span":{"begin":288,"end":293},"obj":"BMO_00480"},{"id":"T13","span":{"begin":298,"end":303},"obj":"BMO_00479"},{"id":"T18","span":{"begin":458,"end":463},"obj":"BMO_00480"},{"id":"T19","span":{"begin":468,"end":473},"obj":"BMO_00479"},{"id":"T21","span":{"begin":523,"end":528},"obj":"BMO_00480"},{"id":"T22","span":{"begin":575,"end":580},"obj":"BMO_00479"},{"id":"T24","span":{"begin":622,"end":627},"obj":"BMO_00479"},{"id":"T26","span":{"begin":636,"end":641},"obj":"BMO_00480"},{"id":"T27","span":{"begin":668,"end":673},"obj":"BMO_00480"},{"id":"T28","span":{"begin":725,"end":730},"obj":"BMO_00479"}],"text":"Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway.\nIn the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle."}

{"project":"Allie","denotations":[{"id":"SS1_19836396_1_0","span":{"begin":141,"end":171},"obj":"expanded"},{"id":"SS2_19836396_1_0","span":{"begin":173,"end":180},"obj":"abbr"}],"relations":[{"id":"AE1_19836396_1_0","pred":"abbreviatedTo","subj":"SS1_19836396_1_0","obj":"SS2_19836396_1_0"}],"text":"Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway.\nIn the silkworm Bombyx mori, densovirus type 1 (BmDNV-1) replicates only in the midgut and causes fatal disease. Resistance to BmDNV-1 is determined by two genes, nsd-1 and Nid-1, respectively. Neither of them has been identified yet. We investigated the viral transcript by RT-PCR in inoculated silkworms carrying different sets of nsd-1 and Nid-1 genotype. BmDNV-1 transcript was not detected in nsd-1-carrying strains irrespective of existence of Nid-1 but clearly detected in strains carrying Nid-1 without nsd-1. The result suggests that nsd-1 blocks early step of infection. On the other hand, Nid-1 does not block cell and nucleus entry and viral transcription in nuclei but blocks later step in the viral infection cycle."}