| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-143 |
DRI_Background |
denotes |
Cell-derived soluble oligomers of human amyloid-beta peptides disturb cellular homeostasis and induce apoptosis in primary hippocampal neurons. |
| T2 |
144-308 |
DRI_Challenge |
denotes |
The concentrations of soluble beta-amyloid (Abeta) oligomers paralleled with the extent of synaptic loss and severity of cognitive impairment in Alzheimer patients. |
| T3 |
309-393 |
DRI_Outcome |
denotes |
However, the neurotoxicity of the naturally generated Abeta species remains unknown. |
| T4 |
394-603 |
DRI_Background |
denotes |
This study was designed to examine the effects of naturally generated Abeta oligomers, secreted from amyloid precursor protein-expressing cells, on the homeostasis and viability of primary hippocampal neurons. |
| T5 |
604-852 |
DRI_Outcome |
denotes |
Our results showed that primary hippocampal neurons incubated with condition media containing cell-secreted soluble Abeta had higher levels of heat-shock protein (HSP)27, HSP60 and HSP70, and lower levels of HSP32 than those of the control neurons. |
| T6 |
853-1049 |
DRI_Background |
denotes |
The cell-secreted soluble Abeta caused mitochondria dysfunction in hippocampal neurons as demonstrated by depolarized membrane potential and decreased cytochrome c oxidase activity and ATP levels. |
| T7 |
1050-1217 |
DRI_Background |
denotes |
The levels of pro-apoptotic proteins, Bid, Bax and cytochrome C, were elevated; whereas anti-apoptotic Bcl-2 protein was reduced in the soluble Abeta-cultured neurons. |
| T8 |
1218-1285 |
DRI_Background |
denotes |
Apoptosis was also evident in these soluble Abeta-cultured neurons. |
| T9 |
1286-1470 |
DRI_Background |
denotes |
These results indicate that naturally secreted Abeta induces neuronal injury/death by activating an apoptotic pathway involving impaired mitochondria function and cellular homeostasis. |
