> top > docs > PubMed:19805545 > annotations

PubMed:19805545 JSONTXT

Annnotations TAB JSON ListView MergeView

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 1163-1174 Cell denotes tumor cells http://purl.obolibrary.org/obo/CL:0001063

sentences

Id Subject Object Predicate Lexical cue
T1 0-89 Sentence denotes Noncatalytic function of ERK1/2 can promote Raf/MEK/ERK-mediated growth arrest signaling.
T2 90-156 Sentence denotes Kinase activity is known as the key biochemical property of MAPKs.
T3 157-265 Sentence denotes Here, we report that ERK1/2 also utilizes its noncatalytic function to mediate certain signal transductions.
T4 266-603 Sentence denotes Sustained activation of the Raf/MEK/ERK pathway induces growth arrest, accompanied by changes in cell cycle regulators (decreased retinoblastoma phosphorylation, E2F1 down-regulation, and/or p21(CIP1) up-regulation) and cell type-specific changes in morphology and expression of c-Myc or RET in the human tumor lines LNCaP, U251, and TT.
T5 604-671 Sentence denotes Ablation of ERK1/2 by RNA interference abrogated all these effects.
T6 672-1064 Sentence denotes However, active site-disabled ERK mutants (ERK1-K71R, ERK2-K52R, and ERK2-D147A), which competitively inhibit activation of endogenous ERK1/2, could not block Raf/MEK-induced growth arrest as well as changes in the cell cycle regulators, although they effectively blocked phosphorylation of the ERK1/2 catalytic activity readouts, p90(RSK) and ELK1, as well as the cell type-specific changes.
T7 1065-1410 Sentence denotes Because this indicated a potential noncatalytic ERK1/2 function, we generated stable lines of the tumor cells in which both ERK1 and ERK2 were significantly knocked down, and we further investigated the possibility using rat-derived kinase-deficient ERK mutants (ERK2-K52R and ERK2-T183A/Y185F) that were not targeted by human small hairpin RNA.
T8 1411-1664 Sentence denotes Indeed, ERK2-K52R selectively restored Raf-induced growth inhibitory signaling in ERK1/2-depleted cells, as manifested by regained cellular ability to undergo growth arrest and to control the cell cycle regulators without affecting c-Myc and morphology.
T9 1665-1766 Sentence denotes However, ERK2-T183A/Y185F was less effective, indicating the requirement of TEY site phosphorylation.
T10 1767-1920 Sentence denotes Our study suggests that functions of ERK1/2 other than its "canonical" kinase activity are also involved in the pathway-mediated growth arrest signaling.

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 396-410 Disease denotes retinoblastoma http://purl.obolibrary.org/obo/MONDO_0008380
T2 571-576 Disease denotes tumor http://purl.obolibrary.org/obo/MONDO_0005070
T3 1163-1168 Disease denotes tumor http://purl.obolibrary.org/obo/MONDO_0005070

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 396-410 Phenotype denotes retinoblastoma HP:0009919
T2 571-576 Phenotype denotes tumor HP:0002664
T3 1163-1168 Phenotype denotes tumor HP:0002664

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 565-570 OrganismTaxon denotes human 9606
T2 1286-1289 OrganismTaxon denotes rat 10114|10116
T4 1386-1391 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 363-373 Body_part denotes cell cycle http://purl.obolibrary.org/obo/UBERON_2000098
T2 887-897 Body_part denotes cell cycle http://purl.obolibrary.org/obo/UBERON_2000098
T3 1603-1613 Body_part denotes cell cycle http://purl.obolibrary.org/obo/UBERON_2000098