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PubMed:19749203 JSONTXT

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Glycan-Motif

Id Subject Object Predicate Lexical cue
T1 245-249 https://glytoucan.org/Structures/Glycans/G46677TE denotes GD1a
T2 245-249 https://glytoucan.org/Structures/Glycans/G69277LC denotes GD1a
T3 1223-1234 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T4 1386-1390 https://glytoucan.org/Structures/Glycans/G46677TE denotes GD1a
T5 1386-1390 https://glytoucan.org/Structures/Glycans/G69277LC denotes GD1a

Inflammaging

Id Subject Object Predicate Lexical cue
T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.
T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 245-249 Glycan_Motif denotes GD1a https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE
T3 1223-1234 Glycan_Motif denotes sialic acid https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY
T4 1386-1390 Glycan_Motif denotes GD1a https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G69277LC|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G46677TE

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
TextSentencer_T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
TextSentencer_T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
TextSentencer_T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
TextSentencer_T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
TextSentencer_T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
TextSentencer_T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
TextSentencer_T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
TextSentencer_T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
TextSentencer_T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
TextSentencer_T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
TextSentencer_T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
TextSentencer_T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
TextSentencer_T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.
T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.
T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 245-249 https://glytoucan.org/Structures/Glycans/G46677TE denotes GD1a
T2 245-249 https://glytoucan.org/Structures/Glycans/G69277LC denotes GD1a
T3 1223-1234 https://glytoucan.org/Structures/Glycans/G81533KY denotes sialic acid
T4 1386-1390 https://glytoucan.org/Structures/Glycans/G46677TE denotes GD1a
T5 1386-1390 https://glytoucan.org/Structures/Glycans/G69277LC denotes GD1a

Glycosmos6-GlycoEpitope

Id Subject Object Predicate Lexical cue
T1 245-249 http://www.glycoepitope.jp/epitopes/EP0056 denotes GD1a
T2 1386-1390 http://www.glycoepitope.jp/epitopes/EP0056 denotes GD1a

GlycoBiology-PACDB

Id Subject Object Predicate Lexical cue
_T1 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC002,LEC056,LEC062,LEC069,LEC081,LEC111,LEC133,LEC171,LEC177,LEC187,LEC211,LEC242,LEC252,LEC258,LEC259,LEC260,LEC262,LEC369,LEC377,LEC422,LEC442,LEC448,LEC450,LEC451,LEC454,LEC472,LEC492,LEC620 denotes Escherichia coli
_T2 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC157,LEC407 denotes Escherichia coli
_T3 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC754 denotes Escherichia coli
_T4 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC243,LEC640 denotes Escherichia coli
_T5 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC295,LEC417 denotes Escherichia coli
_T6 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC487 denotes Escherichia coli
_T7 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC244,LEC256,LEC354 denotes Escherichia coli
_T8 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC054,LEC058,LEC073,LEC082,LEC091,LEC103,LEC109,LEC110,LEC123,LEC158,LEC179,LEC198,LEC205,LEC222,LEC223,LEC224,LEC225,LEC232,LEC298,LEC357,LEC378,LEC383,LEC388,LEC389,LEC397,LEC401,LEC410,LEC452 denotes Escherichia coli
_T9 151-167 http://acgg.asia/db/diseases/pacdb/lec?ids=LEC636 denotes Escherichia coli

ICD10

Id Subject Object Predicate Lexical cue
T1 1568-1578 http://purl.bioontology.org/ontology/ICD10/K65.9 denotes peritoneal
T2 1568-1578 http://purl.bioontology.org/ontology/ICD10/K65 denotes peritoneal

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 15-26 FMAID:82816 denotes ganglioside
_T2 15-26 FMAID:196811 denotes ganglioside
_T3 233-244 FMAID:82816 denotes ganglioside
_T4 233-244 FMAID:196811 denotes ganglioside
_T5 324-329 FMAID:196724 denotes sugar
_T6 507-517 FMAID:196745 denotes isoleucine
_T7 507-517 FMAID:82756 denotes isoleucine
_T8 518-527 FMAID:82765 denotes threonine
_T9 518-527 FMAID:196754 denotes threonine
_T10 791-802 FMAID:82816 denotes ganglioside
_T11 791-802 FMAID:196811 denotes ganglioside
_T12 824-835 FMAID:82816 denotes Ganglioside
_T13 824-835 FMAID:196811 denotes Ganglioside
_T14 929-940 FMAID:82816 denotes ganglioside
_T15 929-940 FMAID:196811 denotes ganglioside
_T16 964-975 FMAID:167685 denotes macrophages
_T17 964-975 FMAID:63261 denotes macrophages
_T18 1027-1039 FMAID:82816 denotes gangliosides
_T19 1027-1039 FMAID:196811 denotes gangliosides
_T20 1275-1286 FMAID:50603 denotes composition
_T21 1275-1286 FMAID:146309 denotes composition
_T22 1333-1344 FMAID:196811 denotes ganglioside
_T23 1333-1344 FMAID:82816 denotes ganglioside
_T24 1535-1546 FMAID:82816 denotes ganglioside
_T25 1535-1546 FMAID:196811 denotes ganglioside
_T26 1568-1590 FMAID:83025 denotes peritoneal macrophages
_T27 1568-1590 FMAID:197284 denotes peritoneal macrophages
_T28 1579-1590 FMAID:167685 denotes macrophages
_T29 1579-1590 FMAID:63261 denotes macrophages
_T30 1639-1651 FMAID:196811 denotes gangliosides
_T31 1639-1651 FMAID:82816 denotes gangliosides
_T32 1740-1752 FMAID:82816 denotes gangliosides
_T33 1740-1752 FMAID:196811 denotes gangliosides
_T34 1949-1960 FMAID:196811 denotes ganglioside
_T35 1949-1960 FMAID:82816 denotes ganglioside
_T36 2039-2050 FMAID:196811 denotes ganglioside
_T37 2039-2050 FMAID:82816 denotes ganglioside
_T38 2051-2058 FMAID:67257 denotes protein
_T39 2051-2058 FMAID:165447 denotes protein

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 151-162 http://purl.bioontology.org/ontology/NCBITAXON/561 denotes Escherichia
T2 1321-1324 http://purl.bioontology.org/ontology/NCBITAXON/604139 denotes non

GO-BP

Id Subject Object Predicate Lexical cue
T1 914-925 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T2 1428-1439 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T3 914-940 http://purl.obolibrary.org/obo/GO_0006689 denotes degradation of ganglioside

GO-MF

Id Subject Object Predicate Lexical cue
T1 27-34 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T2 221-228 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T3 434-441 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T4 662-669 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T5 803-810 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T6 836-843 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T7 1239-1246 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T8 1309-1316 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T9 1325-1332 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T10 1774-1781 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T11 1819-1826 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T12 1961-1968 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T13 27-34 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T14 221-228 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T15 434-441 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T16 662-669 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T17 803-810 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T18 836-843 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T19 1239-1246 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T20 1309-1316 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T21 1325-1332 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T22 1774-1781 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T23 1819-1826 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T24 1961-1968 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T25 27-34 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T26 221-228 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T27 434-441 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T28 662-669 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T29 803-810 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T30 836-843 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T31 1239-1246 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T32 1309-1316 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T33 1325-1332 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T34 1774-1781 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T35 1819-1826 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T36 1961-1968 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T37 27-34 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T38 221-228 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T39 434-441 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T40 662-669 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T41 803-810 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T42 836-843 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T43 1239-1246 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T44 1309-1316 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T45 1325-1332 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T46 1774-1781 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T47 1819-1826 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T48 1961-1968 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T1 10-14 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 245-249 http://www.glycoepitope.jp/epitopes/EP0056 denotes GD1a
PD-GlycoEpitope-B_T2 1386-1390 http://www.glycoepitope.jp/epitopes/EP0056 denotes GD1a

GlyTouCan-IUPAC

Id Subject Object Predicate Lexical cue
GlycanIUPAC_T1 324-329 "http://rdf.glycoinfo.org/glycan/G59665TO" denotes sugar
GlycanIUPAC_T2 324-329 "http://rdf.glycoinfo.org/glycan/G32915EI" denotes sugar
GlycanIUPAC_T3 324-329 "http://rdf.glycoinfo.org/glycan/G60625TS" denotes sugar
GlycanIUPAC_T4 1321-1324 "http://rdf.glycoinfo.org/glycan/G02780QX" denotes non
GlycanIUPAC_T5 1321-1324 "http://rdf.glycoinfo.org/glycan/G18425DX" denotes non
GlycanIUPAC_T6 1321-1324 "http://rdf.glycoinfo.org/glycan/G18630JE" denotes non
GlycanIUPAC_T7 1321-1324 "http://rdf.glycoinfo.org/glycan/G01004IT" denotes non
GlycanIUPAC_T8 1321-1324 "http://rdf.glycoinfo.org/glycan/G87301QZ" denotes non
GlycanIUPAC_T9 1321-1324 "http://rdf.glycoinfo.org/glycan/G39790GW" denotes non
GlycanIUPAC_T10 1321-1324 "http://rdf.glycoinfo.org/glycan/G42928BB" denotes non
GlycanIUPAC_T11 1321-1324 "http://rdf.glycoinfo.org/glycan/G51134HC" denotes non
GlycanIUPAC_T12 1321-1324 "http://rdf.glycoinfo.org/glycan/G68183GR" denotes non
GlycanIUPAC_T13 1321-1324 "http://rdf.glycoinfo.org/glycan/G46883FA" denotes non
GlycanIUPAC_T14 1321-1324 "http://rdf.glycoinfo.org/glycan/G54702VY" denotes non

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 245-249 Glycan denotes GD1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE
T2 1386-1390 Glycan denotes GD1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE
T3 1395-1399 Glycan denotes GM1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR
T4 1622-1627 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 245-249 Glycan denotes GD1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE
T2 1386-1390 Glycan denotes GD1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G46677TE
T3 1395-1399 Glycan denotes GM1a https://api.glycosmos.org/wurcs2image/latest/png/binary/G48558GR
T4 1622-1627 Glycan denotes NeuAc https://api.glycosmos.org/wurcs2image/latest/png/binary/G76685HR

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 245-249 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GD1a http://www.glycoepitope.jp/epitopes/EP0056
T2 1386-1390 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GD1a http://www.glycoepitope.jp/epitopes/EP0056

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 964-975 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T3 1568-1590 Cell denotes peritoneal macrophages http://purl.obolibrary.org/obo/CL:0000581

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 964-975 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T2 1579-1590 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235

GlyCosmos15-Taxon

Id Subject Object Predicate Lexical cue db_id
T1 0-9 Organism denotes Mammalian 40674
T2 52-59 Organism denotes E. coli 562
T3 151-167 Organism denotes Escherichia coli 562

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-105 Sentence denotes Mammalian cell ganglioside-binding specificities of E. coli enterotoxins LT-IIb and variant LT-IIb(T13I).
T2 106-250 Sentence denotes LT-IIb, a type II heat-labile enterotoxin of Escherichia coli, is a potent immunologic adjuvant with high affinity binding for ganglioside GD1a.
T3 251-369 Sentence denotes Earlier study suggested that LT-IIb bound preferentially to the terminal sugar sequence NeuAcalpha2-3Galbeta1-3GalNAc.
T4 370-450 Sentence denotes However, studies in our laboratory suggested a less restrictive binding epitope.
T5 451-613 Sentence denotes LT-IIb(T13I), an LT-IIb variant, engineered by a single isoleucine-threonine substitution, retains biological activity, but with less robust inflammatory effects.
T6 614-823 Sentence denotes We theorized that LT-IIb has a less restrictive binding epitope than previously proposed and that immunologic differences between LT-IIb and LT-IIb (T13I) correlate with subtle ganglioside binding differences.
T7 824-1067 Sentence denotes Ganglioside binding epitopes, determined by affinity overlay immunoblotting and enzymatic degradation of ganglioside components of RAW264.7 macrophages, indicated that LT-IIb bound to a broader array of gangliosides than previously recognized.
T8 1068-1162 Sentence denotes Each possessed NeuAcalpha2-3Galbeta1-3GalNAc, although not necessarily as a terminal sequence.
T9 1163-1287 Sentence denotes Rather, each had a requisite terminal or penultimate single sialic acid and binding was independent of ceramide composition.
T10 1288-1462 Sentence denotes RAW264.7 enterotoxin-binding and non-binding ganglioside epitopes were definitively identified as GD1a and GM1a, respectively, by enzymatic degradation and mass spectroscopy.
T11 1463-1679 Sentence denotes Affinity overlay immunoblots, constructed to the diverse array of known ganglioside structures of murine peritoneal macrophages, established that LT-IIb bound NeuAc- and NeuGc-gangliosides with nearly equal affinity.
T12 1680-1782 Sentence denotes However, LT-IIb(T13I) exhibited enhanced affinity for NeuGc-gangliosides and more restrictive binding.
T13 1783-1969 Sentence denotes These studies further elucidate the binding epitope for LT-IIb and suggest that the diminished inflammatory activity of LT-IIb(T13I) is mediated by a subtle shift in ganglioside binding.
T14 1970-2072 Sentence denotes These studies underscore the high degree of specificity required for ganglioside-protein interactions.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 245-249 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GD1a http://www.glycoepitope.jp/epitopes/EP0056
T2 1386-1390 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes GD1a http://www.glycoepitope.jp/epitopes/EP0056

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 964-975 Body_part denotes macrophages FMA:63261
T2 1568-1590 Body_part denotes peritoneal macrophages FMA:83025

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 52-59 OrganismTaxon denotes E. coli 562
T2 151-167 OrganismTaxon denotes Escherichia coli 562

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 964-975 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235
T2 1579-1590 Body_part denotes macrophages http://purl.obolibrary.org/obo/CL_0000235

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 964-975 Cell denotes macrophages http://purl.obolibrary.org/obo/CL:0000235|http://purl.obolibrary.org/obo/CL:0000394
T3 1568-1590 Cell denotes peritoneal macrophages http://purl.obolibrary.org/obo/CL:0000581