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PubMed_ArguminSci

Id Subject Object Predicate Lexical cue
T1 101-253 DRI_Background denotes INTRODUCTION: Human models of noninvasive breast tumors are limited, and the existing in vivo models do not mimic inter- and intratumoral heterogeneity.
T2 254-346 DRI_Background denotes Ductal carcinoma in situ (DCIS) is the most common type (80%) of noninvasive breast lesions.
T3 347-479 DRI_Background denotes The aim of this study was to develop an in vivo model whereby the natural progression of human DCIS might be reproduced and studied.
T4 480-578 DRI_Approach denotes To accomplish this goal, the intraductal human-in-mouse (HIM) transplantation model was developed.
T5 579-796 DRI_Outcome denotes The resulting models, which mimicked some of the diversity of human noninvasive breast cancers in vivo, were used to show whether subtypes of human DCIS might contain distinct subpopulations of tumor-initiating cells.
T6 797-1039 DRI_Background denotes METHODS: The intraductal models were established by injection of human DCIS cell lines (MCF10DCIS.COM and SUM-225), as well as cells derived from a primary human DCIS (FSK-H7), directly into the primary mouse mammary ducts via cleaved nipple.
T7 1040-1227 DRI_Approach denotes Six to eight weeks after injections, whole-mount, hematoxylin and eosin, and immunofluorescence staining were performed to evaluate the type and extent of growth of the DCIS-like lesions.
T8 1228-1459 DRI_Background denotes To identify tumor-initiating cells, putative human breast stem/progenitor subpopulations were sorted from MCF10DCIS.COM and SUM-225 with flow cytometry, and their in vivo growth fractions were compared with the Fisher's Exact test.
T9 1460-1593 DRI_Background denotes RESULTS: Human DCIS cells initially grew within the mammary ducts, followed by progression to invasion in some cases into the stroma.
T10 1594-1675 DRI_Outcome denotes The lesions were histologically almost identical to those of clinical human DCIS.
T11 1676-1800 DRI_Background denotes This method was successful for growing DCIS cell lines (MCF10DCIS.COM and SUM-225) as well as a primary human DCIS (FSK-H7).
T12 1801-1913 DRI_Background denotes MCF10DCIS.COM represented a basal-like DCIS model, whereas SUM-225 and FSK-H7 cells were models for HER-2+ DCIS.
T13 1914-2050 DRI_Outcome denotes With this approach, we showed that various subtypes of human DCIS appeared to contain distinct subpopulations of tumor-initiating cells.
T14 2051-2299 DRI_Outcome denotes CONCLUSIONS: The intraductal HIM transplantation model provides an invaluable tool that mimics human breast heterogeneity at the noninvasive stages and allows the study of the distinct molecular and cellular mechanisms of breast cancer progression.

c_corpus

Id Subject Object Predicate Lexical cue
T1 15-20 D006801 denotes human
T2 24-29 10090 denotes mouse
T3 24-29 D051379 denotes mouse
T6 75-99 D002285 denotes ductal carcinoma in situ
T7 75-99 D002285 denotes ductal carcinoma in situ
T12 115-120 D006801 denotes Human
T13 143-149 UBERON:0000310 denotes breast
T14 143-156 D001943 denotes breast tumors
T15 143-156 D001943 denotes breast tumors
T18 209-214 CVCL_Z366 denotes mimic
T21 254-278 D002285 denotes Ductal carcinoma in situ
T22 254-278 D002285 denotes Ductal carcinoma in situ
T28 280-284 CVCL_5552 denotes DCIS
T27 280-284 D002285 denotes DCIS
T29 280-284 D002285 denotes DCIS
T30 331-337 UBERON:0000310 denotes breast
T31 436-441 D006801 denotes human
T33 442-446 CVCL_5552 denotes DCIS
T32 442-446 D002285 denotes DCIS
T34 442-446 D002285 denotes DCIS
T35 521-526 D006801 denotes human
T36 530-535 10090 denotes mouse
T37 530-535 D051379 denotes mouse
T38 641-646 D006801 denotes human
T39 659-665 UBERON:0000310 denotes breast
T40 666-673 D009369 denotes cancers
T41 666-673 D009369 denotes cancers
T42 721-726 D006801 denotes human
T44 727-731 CVCL_5552 denotes DCIS
T43 727-731 D002285 denotes DCIS
T45 727-731 D002285 denotes DCIS
T46 773-778 D009369 denotes tumor
T47 773-778 D009369 denotes tumor
T48 862-867 D006801 denotes human
T50 868-872 CVCL_5552 denotes DCIS
T49 868-872 D002285 denotes DCIS
T51 868-872 D002285 denotes DCIS
T54 885-898 CVCL_5552 denotes MCF10DCIS.COM
T55 890-894 D002285 denotes DCIS
T57 890-894 D002285 denotes DCIS
T59 895-898 CHEBI:17905 denotes COM
T60 903-910 CVCL_5593 denotes SUM-225
T61 953-958 D006801 denotes human
T63 959-963 CVCL_5552 denotes DCIS
T62 959-963 D002285 denotes DCIS
T64 959-963 D002285 denotes DCIS
T65 1000-1005 10090 denotes mouse
T66 1000-1005 D051379 denotes mouse
T67 1014-1019 UBERON:0000058 denotes ducts
T68 1032-1038 UBERON:0002030 denotes nipple
T69 1090-1101 D006416 denotes hematoxylin
T70 1090-1101 CHEBI:51686 denotes hematoxylin
T71 1090-1101 CHEBI:5601 denotes hematoxylin
T72 1090-1101 D006416 denotes hematoxylin
T73 1106-1111 1310593 denotes eosin
T74 1106-1111 D004801 denotes eosin
T75 1106-1111 D004801 denotes eosin
T77 1209-1213 CVCL_5552 denotes DCIS
T76 1209-1213 D002285 denotes DCIS
T78 1209-1213 D002285 denotes DCIS
T79 1240-1245 D009369 denotes tumor
T80 1240-1245 D009369 denotes tumor
T81 1273-1278 D006801 denotes human
T82 1279-1285 UBERON:0000310 denotes breast
T85 1334-1347 CVCL_5552 denotes MCF10DCIS.COM
T86 1339-1343 D002285 denotes DCIS
T88 1339-1343 D002285 denotes DCIS
T90 1344-1347 CHEBI:17905 denotes COM
T91 1352-1359 CVCL_5593 denotes SUM-225
T93 1439-1445 76720 denotes Fisher
T92 1439-1445 CVCL_E017 denotes Fisher
T94 1469-1474 D006801 denotes Human
T96 1475-1479 CVCL_5552 denotes DCIS
T95 1475-1479 D002285 denotes DCIS
T97 1475-1479 D002285 denotes DCIS
T98 1520-1525 UBERON:0000058 denotes ducts
T99 1586-1592 UBERON:0003891 denotes stroma
T100 1664-1669 D006801 denotes human
T102 1670-1674 CVCL_5552 denotes DCIS
T101 1670-1674 D002285 denotes DCIS
T103 1670-1674 D002285 denotes DCIS
T105 1715-1719 CVCL_5552 denotes DCIS
T104 1715-1719 D002285 denotes DCIS
T106 1715-1719 D002285 denotes DCIS
T109 1732-1745 CVCL_5552 denotes MCF10DCIS.COM
T110 1737-1741 D002285 denotes DCIS
T112 1737-1741 D002285 denotes DCIS
T114 1742-1745 CHEBI:17905 denotes COM
T115 1750-1757 CVCL_5593 denotes SUM-225
T116 1780-1785 D006801 denotes human
T118 1786-1790 CVCL_5552 denotes DCIS
T117 1786-1790 D002285 denotes DCIS
T119 1786-1790 D002285 denotes DCIS
T122 1801-1814 CVCL_5552 denotes MCF10DCIS.COM
T123 1806-1810 D002285 denotes DCIS
T125 1806-1810 D002285 denotes DCIS
T127 1811-1814 CHEBI:17905 denotes COM
T129 1840-1844 CVCL_5552 denotes DCIS
T128 1840-1844 D002285 denotes DCIS
T130 1840-1844 D002285 denotes DCIS
T131 1860-1867 CVCL_5593 denotes SUM-225
T132 1901-1904 CVCL_1K15 denotes HER
T133 1901-1906 PR:P04626 denotes HER-2
T134 1901-1906 PR:000002082 denotes HER-2
T135 1901-1906 PR:Q03557 denotes HER-2
T136 1901-1906 PR:P34708 denotes HER-2
T137 1901-1906 PR:Q5AK64 denotes HER-2
T139 1908-1912 CVCL_5552 denotes DCIS
T138 1908-1912 D002285 denotes DCIS
T140 1908-1912 D002285 denotes DCIS
T141 1969-1974 D006801 denotes human
T143 1975-1979 CVCL_5552 denotes DCIS
T142 1975-1979 D002285 denotes DCIS
T144 1975-1979 D002285 denotes DCIS
T145 2027-2032 D009369 denotes tumor
T146 2027-2032 D009369 denotes tumor
T147 2146-2151 D006801 denotes human
T148 2152-2158 UBERON:0000310 denotes breast
T149 2273-2279 UBERON:0000310 denotes breast
T150 2273-2286 D001943 denotes breast cancer
T151 2273-2286 D001943 denotes breast cancer

UseCases_ArguminSci_Discourse

Id Subject Object Predicate Lexical cue
T1 0-100 DRI_Background denotes An intraductal human-in-mouse transplantation model mimics the subtypes of ductal carcinoma in situ.
T2 115-253 DRI_Background denotes Human models of noninvasive breast tumors are limited, and the existing in vivo models do not mimic inter- and intratumoral heterogeneity.
T3 254-346 DRI_Background denotes Ductal carcinoma in situ (DCIS) is the most common type (80%) of noninvasive breast lesions.
T4 347-479 DRI_Background denotes The aim of this study was to develop an in vivo model whereby the natural progression of human DCIS might be reproduced and studied.
T5 480-578 DRI_Approach denotes To accomplish this goal, the intraductal human-in-mouse (HIM) transplantation model was developed.
T6 579-796 DRI_Outcome denotes The resulting models, which mimicked some of the diversity of human noninvasive breast cancers in vivo, were used to show whether subtypes of human DCIS might contain distinct subpopulations of tumor-initiating cells.
T7 806-1039 DRI_Background denotes The intraductal models were established by injection of human DCIS cell lines (MCF10DCIS.COM and SUM-225), as well as cells derived from a primary human DCIS (FSK-H7), directly into the primary mouse mammary ducts via cleaved nipple.
T8 1040-1227 DRI_Approach denotes Six to eight weeks after injections, whole-mount, hematoxylin and eosin, and immunofluorescence staining were performed to evaluate the type and extent of growth of the DCIS-like lesions.
T9 1228-1459 DRI_Approach denotes To identify tumor-initiating cells, putative human breast stem/progenitor subpopulations were sorted from MCF10DCIS.COM and SUM-225 with flow cytometry, and their in vivo growth fractions were compared with the Fisher's Exact test.
T10 1469-1593 DRI_Background denotes Human DCIS cells initially grew within the mammary ducts, followed by progression to invasion in some cases into the stroma.
T11 1594-1675 DRI_Outcome denotes The lesions were histologically almost identical to those of clinical human DCIS.
T12 1676-1800 DRI_Background denotes This method was successful for growing DCIS cell lines (MCF10DCIS.COM and SUM-225) as well as a primary human DCIS (FSK-H7).
T13 1801-1913 DRI_Background denotes MCF10DCIS.COM represented a basal-like DCIS model, whereas SUM-225 and FSK-H7 cells were models for HER-2+ DCIS.
T14 1914-2050 DRI_Outcome denotes With this approach, we showed that various subtypes of human DCIS appeared to contain distinct subpopulations of tumor-initiating cells.
T15 2064-2299 DRI_Outcome denotes The intraductal HIM transplantation model provides an invaluable tool that mimics human breast heterogeneity at the noninvasive stages and allows the study of the distinct molecular and cellular mechanisms of breast cancer progression.

PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 115-796 BACKGROUND denotes Human models of noninvasive breast tumors are limited, and the existing in vivo models do not mimic inter- and intratumoral heterogeneity. Ductal carcinoma in situ (DCIS) is the most common type (80%) of noninvasive breast lesions. The aim of this study was to develop an in vivo model whereby the natural progression of human DCIS might be reproduced and studied. To accomplish this goal, the intraductal human-in-mouse (HIM) transplantation model was developed. The resulting models, which mimicked some of the diversity of human noninvasive breast cancers in vivo, were used to show whether subtypes of human DCIS might contain distinct subpopulations of tumor-initiating cells.
T2 806-1460 METHODS denotes The intraductal models were established by injection of human DCIS cell lines (MCF10DCIS.COM and SUM-225), as well as cells derived from a primary human DCIS (FSK-H7), directly into the primary mouse mammary ducts via cleaved nipple. Six to eight weeks after injections, whole-mount, hematoxylin and eosin, and immunofluorescence staining were performed to evaluate the type and extent of growth of the DCIS-like lesions. To identify tumor-initiating cells, putative human breast stem/progenitor subpopulations were sorted from MCF10DCIS.COM and SUM-225 with flow cytometry, and their in vivo growth fractions were compared with the Fisher's Exact test.
T3 1470-2051 RESULTS denotes uman DCIS cells initially grew within the mammary ducts, followed by progression to invasion in some cases into the stroma. The lesions were histologically almost identical to those of clinical human DCIS. This method was successful for growing DCIS cell lines (MCF10DCIS.COM and SUM-225) as well as a primary human DCIS (FSK-H7). MCF10DCIS.COM represented a basal-like DCIS model, whereas SUM-225 and FSK-H7 cells were models for HER-2+ DCIS. With this approach, we showed that various subtypes of human DCIS appeared to contain distinct subpopulations of tumor-initiating cells.
T4 2065-2299 CONCLUSIONS denotes he intraductal HIM transplantation model provides an invaluable tool that mimics human breast heterogeneity at the noninvasive stages and allows the study of the distinct molecular and cellular mechanisms of breast cancer progression.