PubMed:19731076 JSONTXT

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":45,"end":51},"obj":"Gene:733053"},{"id":"T10","span":{"begin":293,"end":299},"obj":"Gene:733053"},{"id":"T11","span":{"begin":329,"end":335},"obj":"Gene:733053"},{"id":"T12","span":{"begin":402,"end":408},"obj":"Gene:733053"},{"id":"T13","span":{"begin":413,"end":419},"obj":"Gene:733053"},{"id":"T14","span":{"begin":424,"end":430},"obj":"Gene:733053"},{"id":"T15","span":{"begin":438,"end":444},"obj":"Gene:733053"},{"id":"T16","span":{"begin":572,"end":578},"obj":"Gene:733053"},{"id":"T17","span":{"begin":586,"end":592},"obj":"Gene:733053"},{"id":"T18","span":{"begin":664,"end":672},"obj":"Chemical:MESH:D001120"},{"id":"T19","span":{"begin":673,"end":679},"obj":"Chemical:MESH:D012694"},{"id":"T2","span":{"begin":58,"end":69},"obj":"Species:7091"},{"id":"T20","span":{"begin":699,"end":705},"obj":"Gene:733053"},{"id":"T21","span":{"begin":713,"end":719},"obj":"Gene:733053"},{"id":"T22","span":{"begin":784,"end":790},"obj":"Gene:733053"},{"id":"T23","span":{"begin":899,"end":905},"obj":"Gene:733053"},{"id":"T24","span":{"begin":984,"end":990},"obj":"Gene:733053"},{"id":"T25","span":{"begin":1067,"end":1073},"obj":"Gene:733053"},{"id":"T26","span":{"begin":1081,"end":1087},"obj":"Gene:733053"},{"id":"T27","span":{"begin":1135,"end":1141},"obj":"Gene:733053"},{"id":"T28","span":{"begin":1149,"end":1155},"obj":"Gene:733053"},{"id":"T29","span":{"begin":1271,"end":1277},"obj":"Gene:733053"},{"id":"T3","span":{"begin":87,"end":97},"obj":"Species:7227"},{"id":"T4","span":{"begin":103,"end":107},"obj":"Gene:41294"},{"id":"T5","span":{"begin":109,"end":113},"obj":"Gene:41294"},{"id":"T6","span":{"begin":198,"end":208},"obj":"Species:7227"},{"id":"T7","span":{"begin":230,"end":233},"obj":"Gene:40940"},{"id":"T8","span":{"begin":253,"end":264},"obj":"Species:7091"},{"id":"T9","span":{"begin":282,"end":286},"obj":"Gene:733053"}],"text":"Molecular cloning and expression analysis of Bmrbp1 , the Bombyx mori homologue of the Drosophila gene rbp1.\nRBP1 is an important splicing factor involved in alternative splicing of the pre-mRNA of Drosophila sex-determining gene dsx. In this work, the Bombyx mori homologue of the rbp1 gene, Bmrbp1, was cloned. The pre-mRNA of Bmrbp1 gene is alternatively spliced to produce four mature mRNAs, named Bmrbp1-PA, Bmrbp1-PB, Bmrbp1-PC and Bmrbp1-PD, with nucleotide lengths of 799 nt, 1,316 nt, 894 nt and 724 nt, coding for 142 aa, 159 aa, 91 aa and 117 aa, respectively. BmRBP1-PA and BmRBP1-PD contain a N terminal RNA recognization motif (RRM) and a C terminal arginine/serine-rich domain, while BmRBP1-PB and BmRBP1-PC only share a RRM. Amino acid sequence alignments showed that BmRBP1 is conserved with its homologues in other insects and with other SR family proteins. The RT-PCR showed that Bmrbp1-PA was strongly expressed in all examined tissues and development stages, but Bmrbp1-PB was weakly expressed in these tissues and stages. The expression of both Bmrbp1-PA and Bmrbp1-PB showed no obvious sex difference. While the Bmrbp1-PC and Bmrbp1-PD were beyond detection by RT-PCR very likely due to their tissue/stage specificity. These results suggested that Bmrbp1 should be a member of SR family splicing factors, whether it is involved in the sex-specific splicing of Bmdsx pre-mRNA needs further research."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":45,"end":51},"obj":"Gene:733053"},{"id":"T2","span":{"begin":58,"end":69},"obj":"Species:7091"},{"id":"T3","span":{"begin":87,"end":97},"obj":"Species:7227"},{"id":"T4","span":{"begin":103,"end":107},"obj":"Gene:41294"},{"id":"T5","span":{"begin":109,"end":113},"obj":"Gene:41294"},{"id":"T6","span":{"begin":198,"end":208},"obj":"Species:7227"},{"id":"T7","span":{"begin":230,"end":233},"obj":"Gene:40940"},{"id":"T8","span":{"begin":253,"end":264},"obj":"Species:7091"},{"id":"T9","span":{"begin":282,"end":286},"obj":"Gene:733053"},{"id":"T10","span":{"begin":293,"end":299},"obj":"Gene:733053"},{"id":"T11","span":{"begin":329,"end":335},"obj":"Gene:733053"},{"id":"T12","span":{"begin":402,"end":408},"obj":"Gene:733053"},{"id":"T13","span":{"begin":413,"end":419},"obj":"Gene:733053"},{"id":"T14","span":{"begin":424,"end":430},"obj":"Gene:733053"},{"id":"T15","span":{"begin":438,"end":444},"obj":"Gene:733053"},{"id":"T16","span":{"begin":572,"end":578},"obj":"Gene:733053"},{"id":"T17","span":{"begin":586,"end":592},"obj":"Gene:733053"},{"id":"T18","span":{"begin":664,"end":672},"obj":"Chemical:MESH:D001120"},{"id":"T19","span":{"begin":673,"end":679},"obj":"Chemical:MESH:D012694"},{"id":"T20","span":{"begin":699,"end":705},"obj":"Gene:733053"},{"id":"T21","span":{"begin":713,"end":719},"obj":"Gene:733053"},{"id":"T22","span":{"begin":784,"end":790},"obj":"Gene:733053"},{"id":"T23","span":{"begin":899,"end":905},"obj":"Gene:733053"},{"id":"T24","span":{"begin":984,"end":990},"obj":"Gene:733053"},{"id":"T25","span":{"begin":1067,"end":1073},"obj":"Gene:733053"},{"id":"T26","span":{"begin":1081,"end":1087},"obj":"Gene:733053"},{"id":"T27","span":{"begin":1135,"end":1141},"obj":"Gene:733053"},{"id":"T28","span":{"begin":1149,"end":1155},"obj":"Gene:733053"},{"id":"T29","span":{"begin":1271,"end":1277},"obj":"Gene:733053"}],"text":"Molecular cloning and expression analysis of Bmrbp1 , the Bombyx mori homologue of the Drosophila gene rbp1.\nRBP1 is an important splicing factor involved in alternative splicing of the pre-mRNA of Drosophila sex-determining gene dsx. In this work, the Bombyx mori homologue of the rbp1 gene, Bmrbp1, was cloned. The pre-mRNA of Bmrbp1 gene is alternatively spliced to produce four mature mRNAs, named Bmrbp1-PA, Bmrbp1-PB, Bmrbp1-PC and Bmrbp1-PD, with nucleotide lengths of 799 nt, 1,316 nt, 894 nt and 724 nt, coding for 142 aa, 159 aa, 91 aa and 117 aa, respectively. BmRBP1-PA and BmRBP1-PD contain a N terminal RNA recognization motif (RRM) and a C terminal arginine/serine-rich domain, while BmRBP1-PB and BmRBP1-PC only share a RRM. Amino acid sequence alignments showed that BmRBP1 is conserved with its homologues in other insects and with other SR family proteins. The RT-PCR showed that Bmrbp1-PA was strongly expressed in all examined tissues and development stages, but Bmrbp1-PB was weakly expressed in these tissues and stages. The expression of both Bmrbp1-PA and Bmrbp1-PB showed no obvious sex difference. While the Bmrbp1-PC and Bmrbp1-PD were beyond detection by RT-PCR very likely due to their tissue/stage specificity. These results suggested that Bmrbp1 should be a member of SR family splicing factors, whether it is involved in the sex-specific splicing of Bmdsx pre-mRNA needs further research."}