PubMed:19665160 JSONTXT

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":66,"end":74},"obj":"Species:7091"},{"id":"T2","span":{"begin":76,"end":87},"obj":"Species:7091"},{"id":"T3","span":{"begin":178,"end":189},"obj":"Species:7091"},{"id":"T4","span":{"begin":299,"end":305},"obj":"Species:9913"},{"id":"T5","span":{"begin":313,"end":316},"obj":"Disease:MESH:D005198"},{"id":"T6","span":{"begin":628,"end":635},"obj":"Species:7091"},{"id":"T7","span":{"begin":660,"end":665},"obj":"Species:271108"},{"id":"T8","span":{"begin":714,"end":718},"obj":"Chemical:-"}],"text":"Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.\nA cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line."}

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":66,"end":74},"obj":"Species:7091"},{"id":"T6","span":{"begin":628,"end":635},"obj":"Species:7091"},{"id":"T7","span":{"begin":660,"end":665},"obj":"Species:271108"},{"id":"T8","span":{"begin":714,"end":718},"obj":"Chemical:-"},{"id":"T2","span":{"begin":76,"end":87},"obj":"Species:7091"},{"id":"T3","span":{"begin":178,"end":189},"obj":"Species:7091"},{"id":"T4","span":{"begin":299,"end":305},"obj":"Species:9913"},{"id":"T5","span":{"begin":313,"end":316},"obj":"Disease:MESH:D005198"}],"text":"Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.\nA cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line."}

{"project":"Allie","denotations":[{"id":"SS1_19665160_1_0","span":{"begin":293,"end":311},"obj":"expanded"},{"id":"SS2_19665160_1_0","span":{"begin":313,"end":316},"obj":"abbr"},{"id":"SS1_19665160_4_0","span":{"begin":527,"end":551},"obj":"expanded"},{"id":"SS2_19665160_4_0","span":{"begin":553,"end":556},"obj":"abbr"},{"id":"SS1_19665160_5_0","span":{"begin":628,"end":658},"obj":"expanded"},{"id":"SS2_19665160_5_0","span":{"begin":660,"end":665},"obj":"abbr"},{"id":"SS1_19665160_6_0","span":{"begin":820,"end":845},"obj":"expanded"},{"id":"SS2_19665160_6_0","span":{"begin":847,"end":850},"obj":"abbr"}],"relations":[{"id":"AE1_19665160_1_0","pred":"abbreviatedTo","subj":"SS1_19665160_1_0","obj":"SS2_19665160_1_0"},{"id":"AE1_19665160_4_0","pred":"abbreviatedTo","subj":"SS1_19665160_4_0","obj":"SS2_19665160_4_0"},{"id":"AE1_19665160_5_0","pred":"abbreviatedTo","subj":"SS1_19665160_5_0","obj":"SS2_19665160_5_0"},{"id":"AE1_19665160_6_0","pred":"abbreviatedTo","subj":"SS1_19665160_6_0","obj":"SS2_19665160_6_0"}],"text":"Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori.\nA cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line."}