| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-80 |
Sentence |
denotes |
PML-IV functions as a negative regulator of telomerase by interacting with TERT. |
| TextSentencer_T2 |
81-163 |
Sentence |
denotes |
Maintaining proper telomere length requires the presence of the telomerase enzyme. |
| TextSentencer_T3 |
164-356 |
Sentence |
denotes |
Here we show that telomerase reverse transcriptase (TERT), a catalytic component of telomerase, is recruited to promyelocytic leukemia (PML) nuclear bodies through its interaction with PML-IV. |
| TextSentencer_T4 |
357-528 |
Sentence |
denotes |
Treatment of interferon-alpha (IFNalpha) in H1299 cells resulted in the increase of PML proteins with a concurrent decrease of telomerase activity, as previously reported. |
| TextSentencer_T5 |
529-656 |
Sentence |
denotes |
PML depletion, however, stimulated telomerase activity that had been inhibited by IFNalpha with no changes in TERT mRNA levels. |
| TextSentencer_T6 |
657-777 |
Sentence |
denotes |
Upon treatment with IFNalpha, exogenous TERT localized to PML nuclear bodies and binding between TERT and PML increased. |
| TextSentencer_T7 |
778-876 |
Sentence |
denotes |
Immunoprecipitation and immunofluorescence analyses showed that TERT specifically bound to PML-IV. |
| TextSentencer_T8 |
877-1020 |
Sentence |
denotes |
Residues 553-633 of the C-terminal region of PML-IV were required for its interaction with the TERT region spanning residues 1-350 and 595-946. |
| TextSentencer_T9 |
1021-1130 |
Sentence |
denotes |
The expression of PML-IV and its deletion mutant, 553-633, suppressed intrinsic telomerase activity in H1299. |
| TextSentencer_T10 |
1131-1263 |
Sentence |
denotes |
TERT-mediated immunoprecipitation of PML or the 553-633 fragment demonstrated that these interactions inhibited telomerase activity. |
| TextSentencer_T11 |
1264-1381 |
Sentence |
denotes |
H1299 cell lines stably expressing PML-IV displayed decreased telomerase activity with no change of TERT mRNA levels. |
| TextSentencer_T12 |
1382-1453 |
Sentence |
denotes |
Accordingly, telomere length of PML-IV stable cell lines was shortened. |
| TextSentencer_T13 |
1454-1559 |
Sentence |
denotes |
These results indicate that PML-IV is a negative regulator of telomerase in the post-translational state. |
| T1 |
0-80 |
Sentence |
denotes |
PML-IV functions as a negative regulator of telomerase by interacting with TERT. |
| T2 |
81-163 |
Sentence |
denotes |
Maintaining proper telomere length requires the presence of the telomerase enzyme. |
| T3 |
164-356 |
Sentence |
denotes |
Here we show that telomerase reverse transcriptase (TERT), a catalytic component of telomerase, is recruited to promyelocytic leukemia (PML) nuclear bodies through its interaction with PML-IV. |
| T4 |
357-528 |
Sentence |
denotes |
Treatment of interferon-alpha (IFNalpha) in H1299 cells resulted in the increase of PML proteins with a concurrent decrease of telomerase activity, as previously reported. |
| T5 |
529-656 |
Sentence |
denotes |
PML depletion, however, stimulated telomerase activity that had been inhibited by IFNalpha with no changes in TERT mRNA levels. |
| T6 |
657-777 |
Sentence |
denotes |
Upon treatment with IFNalpha, exogenous TERT localized to PML nuclear bodies and binding between TERT and PML increased. |
| T7 |
778-876 |
Sentence |
denotes |
Immunoprecipitation and immunofluorescence analyses showed that TERT specifically bound to PML-IV. |
| T8 |
877-1020 |
Sentence |
denotes |
Residues 553-633 of the C-terminal region of PML-IV were required for its interaction with the TERT region spanning residues 1-350 and 595-946. |
| T9 |
1021-1130 |
Sentence |
denotes |
The expression of PML-IV and its deletion mutant, 553-633, suppressed intrinsic telomerase activity in H1299. |
| T10 |
1131-1263 |
Sentence |
denotes |
TERT-mediated immunoprecipitation of PML or the 553-633 fragment demonstrated that these interactions inhibited telomerase activity. |
| T11 |
1264-1381 |
Sentence |
denotes |
H1299 cell lines stably expressing PML-IV displayed decreased telomerase activity with no change of TERT mRNA levels. |
| T12 |
1382-1453 |
Sentence |
denotes |
Accordingly, telomere length of PML-IV stable cell lines was shortened. |
| T13 |
1454-1559 |
Sentence |
denotes |
These results indicate that PML-IV is a negative regulator of telomerase in the post-translational state. |